Identification of a novel esterase from the thermophilic bacterium Geobacillus thermodenitrificans NG80-2

Spatial Metagenomics of Three Geothermal Sites in Pisciarelli Hot Spring Focusing on the Biochemical Resources of the Microbial Consortia

“…Research on extremophiles and their enzymes (extremozymes) has not only reshaped our understanding of the origin and evolution of life [ 5 ] and the potential for life on other planetary bodies [ 6 ], but also it has simultaneously led to numerous advances in molecular biology, medicine, and biotechnology [ 7 , 8 , 9 , 10 ]. In fact, extremozymes represent interesting cases of protein adaptation under conditions where conventional enzymes quickly denature [ 11 , 12 , 13 ]. Thus, extremozymes are ideal tools for industrial applications where harsh chemical and physical conditions are encountered.…”

Identification and Biochemical Characterization of a Novel Hormone-Sensitive Lipase Family Esterase Est19 from the Antarctic Bacterium Pseudomonas sp. E2-15

“…Especially, in the presence of 20% formaldehyde and ethanol, the residual enzyme activity was only 1.1–1.5%. In contrast, DMSO had little effect on the activity of Est19, as observed for other esterases [ 6 , 8 ]. Metal ions can have different effects on the activity of esterases.…”

“…The other inhibitors PMSF, SDS, and TWEEN 80 remarkably inhibited enzyme Est19 ( Table 2 ). The inhibition effect of PMSF suggests that a serine residue was involved in the catalytic site such as for other esterases [ 8 , 9 ]. The effect of organic reagents on enzyme activity was generally negative ( Table 2 ).…”

“…Lipolytic enzymes are widely distributed in nature (i.e., animals, plants, and microorganisms), and a relatively large number has been isolated from metagenomes and bacteria [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. The latter includes enzymes with different biological activities isolated from genera such as Pseudomonas [ 1 ], Psychrobacter [ 5 ], Salinisphaera [ 9 ], Serratia [ 11 ], Burkholderia [ 12 ], and Staphylococcus [ 13 ].…”

Revisiting the scope and applications of food enzymes from extremophiles

“…For instance, Candida antarctica lipase B (Cal B) acts as a lipase when adsorbed to an acylglyceride interface and as TA B L E 3 Some esterase producing extremophiles an esterase when exposed to an aqueous environment (Benson & Pleiss, 2017). Several microbial esterases from extreme environments (Table 3) are very active at low and high temperature, salt concentration, and pH (Adıgüzel, 2020;Curci et al, 2019;Jiang, Zhang, Gao, & Hu, 2016;Polkade, Ramana, Joshi, Pardesi, & Shouche, 2015;Wang, Wang, Cao, & Zhu, 2016;Zarafeta et al, 2016).…”