Identification of a Novel Family of Growth Hormone-Related Proteins Secreted by Rat Placenta

Ogilvie, Susan; Buhi, William C.; Olson, John A.; Shiverick, Kathleen T.

doi:10.1210/endo-126-6-3271

Cited by 43 publications

(26 citation statements)

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“…However, this is not the case with PL-a in view of the reactivities with the antisera we observed in this and our previous study, which showed that there is a distinct mRNA corresponding to PL-a in rat placenta [12]. Antisera directed against the synthetic peptides of PLs and PLPs have been found to be useful tools with which to investigate the characteristics of PLs and PLPs [13][14][15][16][27][28][29][30][31]. In this study, PL-a was recognized by the All-86 antiserum based on the amino acid sequence of PL-II, but it did not react with AI-86, which bound to PL-I.…”

Section: Discussionmentioning

confidence: 93%

Characterization of Rat Placental Lactogen-.ALPHA. (PL-.ALPHA.) with an Antipeptide Antibody Directed against Rat PLs.

Hattori¹,

Wakimasu

Takahashi

et al. 1993

Endocr J

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Abstract. We have already reported that the serum of the mid-pregnant rat contains placental lactogen-a (PL-a), which has a higher molecular weight than PL-I when analysed by gel-filtration chromatography. In order to characterize PL-a, we prepared antipeptide antisera directed against the hydrophilic regions of authentic PLs and developed RIAs with them. With these RIAs, antiserum AI-86 reacted only with PL-I, whereas antiserum AI I-86 reacted with both PL-a and PL-I, which indicates that the antibody recognition sites of these molecules are not identical and their core proteins differ distinctly. As the use of the RIAs with AI-86 and All-86 in combination enabled PL-a and PL-I to be discriminated, the glycoresidues and hydrophobicity of PL-a were analyzed further. Analysis with hydrophobic chromatography revealed that PL-a was less hydrophobic than PL-I and, furthermore, PL-a, but not PL-I, bound specifically to a wheat germ agglutinin (WGA) lectin column. Therefore, in view of their different amino acid sequences and glycoresidues, PL-a and PL-I are distinct molecular entities.

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Section: Discussionmentioning

confidence: 93%

Characterization of Rat Placental Lactogen-.ALPHA. (PL-.ALPHA.) with an Antipeptide Antibody Directed against Rat PLs.

Hattori¹,

Wakimasu

Takahashi

et al. 1993

Endocr J

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“…GH variants are, however, present in the ovine (Lacroix et al 1996) and rodent (Ogilvie et al 1990) placentae, and GH mRNA has been detected in the ovine placenta, particularly in trophoectoderm and syncytium (Lacroix et al 1999).…”

Section: The Placental Mini-hypophysismentioning

confidence: 99%

Growth hormone: roles in female reproduction

Hull¹,

Harvey²

2001

Journal of Endocrinology

197

124

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GH, as its name suggests, is obligatory for growth and development. It is, however, also involved in the processes of sexual differentiation and pubertal maturation and it participates in gonadal steroidogenesis, gametogenesis and ovulation. It also has additional roles in pregnancy and lactation. These actions may reflect direct endocrine actions of pituitary GH or be mediated by its induction of hepatic or local IGF-I production. However, as GH is also produced in gonadal, placental and mammary tissues, it may act in paracrine or autocrine ways to regulate local processes that are strategically regulated by pituitary GH. The concept that GH is an important modulator of female reproduction is the focus of this review.

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“…Growth of the placenta is regulated by several growth factors, including placental growth factor (PlGF), vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 [1, 14, 15]. These growth factors are responsible for fetal development as well as placenta growth [5,9,20,22]. PlGF is a polypeptide growth factor that shares a 53% amino acid sequence homology with the platelet-derived growth factor domain of VEGF [17].…”

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confidence: 99%

Streptozotocin-Induced Diabetes Decreases Placenta Growth Factor (PIGF) Levels in Rat Placenta

Koh

Sung

Won

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The placenta produces several growth factors, including placenta growth factor (PlGF), which are essential for placenta growth and fetal growth. Diabetic pregnancy induces the abnormal placental growth and fetal development. This study investigated whether diabetes in pregnant rats induces changes in PlGF expression in the placenta. Diabetes was induced by a single intravenous injection of streptozotocin (35 mg/kg body weight) on day 0 of pregnancy, blood and tissue samples were collected on day 20 of pregnancy. In the diabetic group, maternal body weight and fetal weight significantly decreased compared to controls. RT-PCR and Western blot analyses showed that expression of PlGF was significantly decreased in placenta by streptozotocin treatment. Immunohistochemical study showed that the positive signal of PlGF in trophoblast cells was decreased in the diabetic group compared to controls. These findings demonstrate the decline of PlGF in the placenta in diabetic pregnancy. KEY WORDS: diabetes, placenta, PlGF.J. Vet. Med. Sci. 69(9): 877-880, 2007 The placenta is a critical organ for both fetal development and the maintenance of pregnancy. Growth of the placenta is regulated by several growth factors, including placental growth factor (PlGF), vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 [1, 14, 15]. These growth factors are responsible for fetal development as well as placenta growth [5,9,20,22]. PlGF is a polypeptide growth factor that shares a 53% amino acid sequence homology with the platelet-derived growth factor domain of VEGF [17]. Unlike VEGF, abundant expression of PlGF is restricted to the placenta [18]. PlGF was formerly known as a potent angiogenic growth factor capable of inducing the proliferation, migration, and activation of endothelial cells [17]. PlGF plays an important endocrinological and nutritional role, and contributes to the regulation of placental function.Gestational diabetes is one of the most common complications of pregnancy, and is associated with both abnormal placental growth and fetal development [3,16,19]. Severely diabetic pregnancy can induce abortion and prematurity [3,19]. Recently, apoptotic cell death was increased in the placenta of pregnant women with gestational diabetes [21]. PlGF acts as an anti-apoptotic factor for trophoblast in vitro [11]. Therefore, we propose that diabetic pregnancy induces change in PlGF expression and this dysregulation may affect placental development. However, little data is available on the expression of PlGF in the placenta tissue in diabetic pregnancy. Therefore, the present study was performed to provide this information. MATERIALS AND METHODSExperimental animals: Female Sprague-Dawley rats (200-220 g, n=30) were purchased from Samtako Co. (Laboratory Animal Breeding Center, Korea), randomly divided into 2 groups, control group and diabetic group (n=15 per group). Animals were maintained under controlled temperature (25°C) and lighting (14/10 light/dark cycle), and were allowed to have free access...

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Identification of a Novel Family of Growth Hormone-Related Proteins Secreted by Rat Placenta

Cited by 43 publications

References 0 publications

Characterization of Rat Placental Lactogen-.ALPHA. (PL-.ALPHA.) with an Antipeptide Antibody Directed against Rat PLs.

Characterization of Rat Placental Lactogen-.ALPHA. (PL-.ALPHA.) with an Antipeptide Antibody Directed against Rat PLs.

Growth hormone: roles in female reproduction

Streptozotocin-Induced Diabetes Decreases Placenta Growth Factor (PIGF) Levels in Rat Placenta

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