2004
DOI: 10.1158/0008-5472.can-03-0849
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Identification of a Novel Mammary-Restricted Cytochrome P450, CYP4Z1, with Overexpression in Breast Carcinoma

Abstract: By screening a transcriptome database for expressed sequence tags that are specifically expressed in mammary gland and breast carcinoma, we identified a new human cytochrome P450 (CYP), termed CYP4Z1. The cDNA was cloned from the breast carcinoma line SK-BR-3 and codes for a protein of 505 amino acids. Moreover, a transcribed pseudogene CYP4Z2P that codes for a truncated CYP protein (340 amino acids) with 96% identity to CYP4Z1 was found in SK-BR-3. CYP4Z1 and CYP4Z2P genes consisting of 12 exons are localized… Show more

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Cited by 96 publications
(87 citation statements)
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“…CYP2W1 has been shown to be almost exclusively expressed during embryogenesis and in adult humans it is mainly detected in tumour tissue samples, more frequently from colon and adrenal gland (Karlgren et al, 2005, submitted). With respect to CYP4Z1, it is regulated by the glucocorticoid and progesterone receptors and has been shown to be overexpressed preferentially in breast carcinoma tissue and mammary gland (Rieger et al, 2004;Savas et al, 2005).…”
Section: Oh-docmentioning
confidence: 99%
“…CYP2W1 has been shown to be almost exclusively expressed during embryogenesis and in adult humans it is mainly detected in tumour tissue samples, more frequently from colon and adrenal gland (Karlgren et al, 2005, submitted). With respect to CYP4Z1, it is regulated by the glucocorticoid and progesterone receptors and has been shown to be overexpressed preferentially in breast carcinoma tissue and mammary gland (Rieger et al, 2004;Savas et al, 2005).…”
Section: Oh-docmentioning
confidence: 99%
“…The sonicate was centrifuged at 4000 ϫ g for 10 min to obtain the nuclear pellet. From the resulting supernatant, the mitochondrial fraction was extracted by further centrifugation at 10,000 ϫ g for 10 min (38) in an ultracentrifuge (SW55Ti rotor, optima XL-110K; Beckman Coulter). The postmitochondrial supernatant was further centrifuged at 100,000 ϫ g for 30 min to isolate the microsomal fraction as a pellet and the supernatant obtained was the cytosolic fraction.…”
Section: Subcellular Fractionationmentioning
confidence: 99%
“…Recently, the sequence data in the public databases and the tools of bioinformatics have provided researchers excellent and time-saving approaches to identify genes that could be used as tumor diagnostic markers, prognostic indicators, and suitable targets for various forms of therapeutic intervention [6][7][8]. In the public database, expressed sequence tags (EST) are the most major sources to identify genes expressed in a variety of human tissues and in different pathological situations.…”
Section: Introductionmentioning
confidence: 99%