Identification of a novel marker and its associated laccase gene for regulating ear length in tropical and subtropical maize lines

Abstract

Key message This study revealed the identification of a novel gene, Zm00001d042906, that regulates maize ear length by modulating lignin synthesis and reported a molecular marker for selecting maize lines with elongated ears. Abstract Maize ear length has garnered considerable attention due to its high correlation with yield. In this study, six maize inbred lines of significant importance in maize breeding were used as parents. The temperate maize inbred line Ye107, characterized by a short ear, was crossed with five tropical or subtropical inbred lines featuring longer ears, creating a multi-parent population displaying significant variations in ear length. Through genome-wide association studies and mutation analysis, the A/G variation at SNP_183573532 on chromosome 3 was identified as an effective site for discriminating long-ear maize. Furthermore, the associated gene Zm00001d042906 was found to correlate with maize ear length. Zm00001d042906 was functionally annotated as a laccase (Lac4), which showed activity and influenced lignin synthesis in the midsection cells of the cob, thereby regulating maize ear length. This study further reports a novel molecular marker and a new gene that can assist maize breeding programs in selecting varieties with elongated ears.

Identification of candidate gene associated with maize northern leaf blight resistance in a multi-parent population

“…The best model was selected based on the λ values of GWAS in the MPP, and then the selected model was applied to the MPP which consisted of 797 families, and for each RIL subpopulation that contained approximately 200 families (Shi et al 2022 ). Candidate genes were screened within a 50 kb range upstream and downstream of the SNPs (Jiang et al 2023 ; Bi et al 2024 ). Linkage mapping was performed for each of the four RIL subpopulations, and SNPs showing no polymorphism between the two parental lines of each RIL subpopulation were filtered out.…”