Identification of a novel murine organic anion transporter like protein 1 (OATLP1) expressed in the kidney

Abstract

The organic anion transporters (OATs) are expressed in various tissues, primarily in the kidney and liver, but they are also expressed in the placenta, small intestine, and the choroid plexus, which are all epithelial tissues that transport xenobiotics. Six isoforms of OATs are currently known. Considering the variety of organic anionic compounds, other OATs isoforms can be assumed. In this connection, we have searched for a new isoform in the expressed sequence tag (EST) database. We found the new candidate clone AK052752 in the mouse kidney cDNA library and we named it mouse organic anion transporter like protein 1 (mOATLP1). The mOATLP1 cDNA consisted of 2221 base pairs that encoded a 552 amino acid residue protein with 12 putative transmembrane domains. The deduced amino acid sequence of mOATLP1 showed 37 to 63% identity to other members of the OAT family. According to the tissue distribution based on Northern blot analysis, 2.7 kb and 2.9 kb mOATLP1 transcripts (approximate sizes) were observed in the kidney and liver. An 85-kDa band (approximate) was detected using Western blot analysis of mouse kidney performed with a synthesized oligopeptide-induced mOATLP1 antibody. Immunohistochemical results showed mOATLP1 was stained in the blood vessels, glomeruli (the parietal epithelial cells and podocytes), distal convoluted tubules, connecting tubules, and inner medullary collecting tubules. mOATLP1 appears to be a novel candidate for an organic anion transporter isoform identified in the kidney.

Transport of Inorganic Mercury and Methylmercury in Target Tissues and Organs

“…Currently, only OAT2 and OAT10 have been detected in the intestine (Hilgendorf et al, 2007; Bahn et al, 2008); the membrane localization of either protein has not been examined. In addition, several members of the OATP family (Hilgendorf et al, 2007) and a novel organic anion transporter-like protein (OATLP1) are present in the intestine (Jung et al, 2006). Currently, there are no published data regarding the ability of these carriers to transport mercuric ions.…”

Na+-Ca2+ exchanger modulates Ca2+ content in intracellular Ca2+ stores in rat osteoblasts

“…We used the following primer pairs for PCR amplification: ALPforward 5'-ATGCTTCGACTAAGTCTCCCACCCA-A-3'; reverse 5'-AAAGATGGGTCATGGGGTTCC-CAA-3'. The PCR products were resolved on 1% agarose gels stained with ethidium bromide, and photographed (Jung et al, 2006).…”

Podocyte Expression of Membrane Transporters Involved in Puromycin Aminonucleoside-Mediated Injury

“…The membrane transporters OATLP1 and mOCTL1 have been described in podocytes, and have been shown to contribute to trans-epithelial molecular transfer in these cells [2], [3]. More recently, Xia e al.…”

“…In the kidney, several members of the main membrane transporter families have been described in the renal tubule in the context of ion and drug transport [1]. Recently, two transporters with broad specificity for small molecules have been identified in mouse podocytes in relation to drug clearance [2], [3].…”