Identification of a protein associated with the activity of cytokine‑induced killer cells

Abstract

Abstract. Cytokine-induced killer cells (CIKs) adoptive immunotherapy for efficient antitumor ability is used clinically, but details regarding the proteins associated with CIK activity remain unclear. In the current study, the cytotoxicity of CIKs on hepatoma was identified to be significantly downregulated by 1.61-fold following gentamincin treatment. Further research revealed that a differentially expressed protein (P43) was significantly downregulated by 1.22-fold using one-dimensional gel electrophoresis analysis. Of these, the P43 was identified as human haptoglobin using liquid chromatography-mass spectrometry. Western blotting demonstrated that the haptoglobin specifically reacted with rabbit anti-human-haptoglobin. Furthermore, western blotting results verified that the haptoglobin was significantly downregulated by 1.17-fold compared with the control group. In addition, the expression of haptoglobin mRNA was significantly downregulated by 1.73-fold following gentamincin treatment. Taken together, the results of the present study demonstrated that the expression of haptoglobin protein was associated with the activity of CIKs, and the results will be beneficial to the further investigation of CIK activity-enhancement mechanism.

Identification of an activation‑related protein in B cells in the ABO incompatible condition

“…Previous research has identified that haptoglobin is associated with the activation of cytokine-induced killer cells (CIKs) (12), and B cells in peripheral blood mononuclear cells (PBMCs) from blood group A could be activated by HK2 cells carrying blood group B antigen (13). In the present study, the role of HP in B-cell activation was analyzed by flow cytometry, dot-ELISA and bioinformatics.…”