Identification of a putative cellular receptor for bovine herpesvirus 1

Thaker, Subhashchandra R.; Stine, Douglas L.; Zamb, Timothy J.; Srikumaran, Subramaniam

doi:10.1099/0022-1317-75-9-2303

Cited by 19 publications

(16 citation statements)

References 30 publications

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“…The virus receptor(s) for BoHV-1 has not yet been well defined. Two proteins as the candidate receptors with molecular weight of 56 and 60 kDa had been reported (Thaker et al, 1994;Varthakavi and Minocha, 1996). But the amino acid sequence for the two proteins is unknown.…”

Section: Discussionmentioning

confidence: 99%

Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells

Zhu

Ding

Tao

et al. 2010

Veterinary Microbiology

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Cholesterol is involved in the life cycle of many viruses. Here, we examined the role of cholesterol for both viral envelope and target cell membrane for bovine herpesvirus type 1 (BoHV-1) infection. Cholesterol depletion by pretreatment of Madin-Darby bovine kidney (MDBK) cells with a cholesterol-sequestering drug methyl-beta-cyclodextrin (MbetaCD), inhibited the production of BoHV-1 in a dose-dependent manner. This inhibitory effect was partially reversed by cholesterol replenishment, indicating that the reduction was caused by cholesterol depletion. Cholesterol depletion at the post-entry stage only had a mild effect on the virus production. However, cell membrane cholesterol depletion did not reduce the virus attachment. In addition, treatment of BoHV-1 particles with MbetaCD also reduced the virus infectivity significantly and the effect was partially reversed by addition of exogenous cholesterol. Taken together, these data implicated that cell membrane cholesterol mainly contributed to BoHV-1 entry into MDBK cells and the viral envelope cholesterol was also essential for the virus infectivity.

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Section: Discussionmentioning

confidence: 99%

Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells

Zhu

Ding

Tao

et al. 2010

Veterinary Microbiology

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“…The cell surface proteins of P815 and 2B2 cells were labeled with 125 I (Perkin-Elmer Life Sciences, Boston, Mass. ), using the lactoperoxidase method as described previously (34). Briefly, 5 ϫ 10 7 cells were washed three times in cold PBS and resuspended in 1 ml of 20 mM glucose (Sigma) in PBS (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Bovine CD18 Is Necessary and Sufficient To Mediate Mannheimia ( Pasteurella ) haemolytica Leukotoxin-Induced Cytolysis

Deshpande

Ambagala

et al. 2002

Infect Immun

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Leukotoxin (Lkt) secreted by Mannheimia (Pasteurella) haemolytica is an RTX toxin which is specific for ruminant leukocytes. Lkt binds to ␤ 2 integrins on the surface of bovine leukocytes. ␤ 2 integrins have a common ␤ subunit, CD18, that associates with three distinct ␣ chains, CD11a, CD11b, and CD11c, to give rise to three different ␤ 2 integrins, CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), and CD11c/CD18 (CR4), respectively. Our earlier studies revealed that Lkt binds to all three ␤ 2 integrins, suggesting that the common ␤ subunit, CD18, may be the receptor for Lkt. In order to unequivocally elucidate the role of bovine CD18 as a receptor for Lkt, a murine cell line nonsusceptible to Lkt (P815) was transfected with cDNA for bovine CD18. One of the transfectants, 2B2, stably expressed bovine CD18 on the cell surface. The 2B2 transfectant was effectively lysed by Lkt in a concentration-dependent manner, whereas the P815 parent cells were not. Immunoprecipitation of cell surface proteins of 2B2 with monoclonal antibodies specific for bovine CD18 or murine CD11a suggested that bovine CD18 was expressed on the cell surface of 2B2 as a heterodimer with murine CD11a. Expression of bovine CD18 and the Lkt-induced cytotoxicity of 2B2 cells were compared with those of bovine polymorphonuclear neutrophils and lymphocytes. There was a strong correlation between cell surface expression of bovine CD18 and percent cytotoxicity induced by Lkt. These results indicate that bovine CD18 is necessary and sufficient to mediate Lkt-induced cytolysis of target cells.Mannheimia (Pasteurella) haemolytica serotype 1 is the major bacterial pathogen of bovine pneumonic pasteurellosis, an acute fibrinous pleuropneumonia, which causes extensive economic losses to the cattle industry in North America and other parts of the world (4). M. haemolytica A1 is commonly found in the tonsillar crypts and the upper respiratory tracts of healthy cattle (9). In conjunction with active viral infection and stress factors, M. haemolytica migrates to the lungs, where it multiplies rapidly (7,26). M. haemolytica produces several virulence factors, of which the extracellular leukotoxin (Lkt) is considered the most important one responsible for leukocyte damage in the lung (3, 27). Lkt-induced neutrophil lysis and degranulation have been implicated as the primary causes of the acute inflammation characteristic of pneumonic pasteurellosis (31).Leukotoxin (Lkt) is a 102-kDa glycoprotein which is produced during the logarithmic phase of bacterial growth in vitro (2, 28). Lkt belongs to the family of RTX (repeats in toxins) toxins and shares extensive homology with the exotoxins produced by other gram-negative bacteria such as Escherichia coli (33), Actinobacillus pleuropneumoniae (8), and Actinobacillus actinomycetemcomitans (17). Despite the extensive homology shared by the RTX family, there is a marked dichotomy among the members of the family with respect to target cell specificity. The toxins secreted by E. coli and A. pleuropneumoniae are lytic to erythrocyte...

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“…2). Although the data presented in this study along with another recent report (Thaker et al, 1994) indicate a second receptor other than cell surface heparan sulphate, it is not known whether these two receptor proteins are of a similar nature or are involved in membrane fusion triggered by virus infection. Haywood (1994) reported evidence for sequential interaction of VAPs with the cell surface receptors.…”

Section: Ee~ee~eee:ee:ee~e:ee:~e~eeeeeee~e2ee@ee~e~ee~}~ee~i~ehe~ee2ementioning

confidence: 52%

“…The study also suggested that gB, gC and gD may recognize different binding sites (receptors) on the surfaces of permissive cells. For BHV-1 at least two different cellular receptors have been identified, one of which was a heparin-like moiety that was found to interact specifically with gC (Okazaki et al, 1991) and the other that reacted with gD (Thaker et al, 1994). Whether these receptors act as co-receptors or in a sequential manner has not been determined.…”

Section: Introductionmentioning

confidence: 99%

Identification of a 56 kDa putative bovine herpesvirus 1 cellular receptor by anti-idiotype antibodies

Varthakavi

Minocha

1996

Journal of General Virology

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Identification of a putative cellular receptor for bovine herpesvirus 1

Cited by 19 publications

References 30 publications

Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells

Critical role of cholesterol in bovine herpesvirus type 1 infection of MDBK cells

Bovine CD18 Is Necessary and Sufficient To Mediate Mannheimia ( Pasteurella ) haemolytica Leukotoxin-Induced Cytolysis

Identification of a 56 kDa putative bovine herpesvirus 1 cellular receptor by anti-idiotype antibodies

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