Identification of a Recombinant HTLV-II Envelope Protein for Serological Detection of HTLV-II Carriers

Chen, Yi-Ming A.; Essex, Max

doi:10.1089/aid.1991.7.453

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…Recently, Kaplan et al screened 75 spider monkeys by an HTLV-I enzyme immunoassay (EIA) and found none of them to be seropositive (28). It is possible that the anti-STLV-II antibodies present in the monkey sera were missed by the HTLV-I antigen-based EIA since some HTLV-II-positive serum samples have been missed by such screening tests (5,21). In addition, the use of anti-human immunoglobulin G in the HTLV-I EIAs may raise concerns regarding the detectability of monkey antibodies in the serum samples.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, instead of using HTLV-I EIAs, we used an HTLV-II recombinant envelope protein, RP-IIB, in a Western blot assay to detect STLV-II infection in New World monkeys. This assay has been proved to be able to detect virtually all HTLV-II carriers, including several intravenous drug users who were seronegative to HTLV-I EIAs (5,7). In order to cross-react with monkey antibodies, the concentration of biotinylated sheep anti-human immunoglobulin used in the RP-IIB Western blot assay was raised from a dilution of 1/500 to one of 1/100.…”

Section: Discussionmentioning

confidence: 99%

“…HTLV-I and HTLV-II recombinant proteins. Two recombinant proteins (RP-B1 and RP-IIB) described previously were used to analyze the antibody reactivities of monkey sera by Western blot assays (5,6). RP-B1 contains amino acid residues 166 to 201 of the HTLV-I exterior glycoprotein gp46, and RP-IIB contains amino acid residues 96 to 235 of HTLV-II exterior glycoprotein gp46.…”

Section: Materuils and Methodsmentioning

confidence: 99%

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Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys

Chen

Jang

Kanki

et al. 1994

J Virol

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Since the description of human T-cell leukemia virus type I (HTLV-I) and its simian counterpart, simian T-cell leukemia virus type I (STLV-I), the possible existence of other related simian retroviruses has been raised. Here, we report a new retrovirus, STLV-II, which we have identified in spider monkeys (Ateles fusciceps), a New World primate species. Initially, a recombinant HTLV-II envelope protein (RP-IIB) was used to identify anti-STLV-II antibodies in New World monkeys by Western blot (immunoblot) assays. Subsequently, the virus was characterized by Southern blot hybridization, which showed that STLV-II and HTLV-II have a high degree of nucleotide sequence homology but have different restriction enzyme patterns. Nucleotide sequence analysis of the pX-II region of STLV-II provirus revealed 3% variation with the corresponding region of HTLV-II. Electron micrographic studies revealed HTLV-like, type C retrovirus particles outside the cell membranes of STLV-II-infected cells. This study describes the first link between HTLV-II and a simian reservoir in the New World. Further molecular studies of STLV-II infection in different species of New World monkeys, especially from the wild, may provide valuable information about the origin and intragroup relationships of South American monkeys. Spider monkeys infected with STLV-II may serve as an important animal model for HTLV-II infection in humans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Materuils and Methodsmentioning

confidence: 99%

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Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys

Chen

Jang

Kanki

et al. 1994

J Virol

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“…Most studies were restricted to production of a few different segments of the Env protein. Initial studies described the expression of truncated gp21 and gp46 protein segments (35)(36)(37)(38), while subsequent studies described the production of different gp21 and gp46 immunogenic regions in fusion with glutathione S-transferase (GST) (39,40). In addition, a fusion protein comprising sections of p24 (residues 14 to 139) and gp46 (residues 197 to 295) was produced and shown to discriminate HTLV-1-positive from HTLV-1-negative samples (41).…”

mentioning

confidence: 99%

Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients

et al. 2021

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HTLV-1 can cause life-threatening diseases for which there are no effective treatments. Prevention of HTLV-1 infection requires massive testing of pregnant women, blood for transfusion and, organs for transplantation as well as safe sex. In this context, serological assays are widely used for monitoring HTLV-1 infections. Despite the necessity of recombinant antigens to compose serological tests, there is little information available on procedures to produce recombinant HTLV1/2 antigens for serological diagnostic purposes. In this work, we tested a series of genetic constructions to select those more amenable for production in bacterial systems. To overcome the constraints to express sections of viral envelope proteins in bacteria, we have used the p24 segment of the gag protein as a scaffold to display the immunogenic regions of gp46 and gp21. Nine recombinant antigenic proteins derived from HTLV-1 and five derived from HTLV-2 were successfully purified. The HTLV-1 antigens showed high efficiency in discriminating HTLV-positive from HTLV-negative samples using ELISA. Interestingly, HTLV-1-positive samples showed a high level of cross-reaction with HTLV-2 antigens. This finding is explained by the high sequence conservation between the structural proteins of these two highly related viruses. In summary, the results presented in this work provide a detailed description of the methods used to produce recombinant HTLV-1 and HTLV-2 antigens and demonstrate that the HTLV-1 antigens show strong potential for serological diagnosis of HTLV1 infections.

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Synthetic peptide-based immunoassay for the detection of antibodies to human T-lymphotropic virus types I and II

Su¹,

Coleman²,

Morgan³

et al. 1994

Peptides

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Identification of a Recombinant HTLV-II Envelope Protein for Serological Detection of HTLV-II Carriers

Cited by 4 publications

References 17 publications

Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys

Isolation and characterization of simian T-cell leukemia virus type II from New World monkeys

Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients

Synthetic peptide-based immunoassay for the detection of antibodies to human T-lymphotropic virus types I and II

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