Identification of a S6 kinase transcript in the Chinese mitten crab Eriocheir sinensis and its molting-related expression in muscle tissues

Tian, Zhong; Lin, Guangchun; Jiao, Chuanzhen

doi:10.1007/s12562-019-01326-y

Cited by 4 publications

(6 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ActRI is the interacting protein of ActRIIB binding to myostatin ( Hata & Chen, 2016 ), and its lower expression was observed with interference of Es-ActRIIB in this study; the expressions of downstream transcription factors SMAD3 and SMAD4 were not influenced, the reason probably was that SMAD s, as core and versatile cytokines, are active in the TGF-β pathway, including the signal transduction of TGF-β/BMP/activin, not only in myostatin/ ActRIIB pathway ( Massague, Seoane & Wotton, 2005 ; Tian, Jiao & Cheng, 2018b ). Moreover, in the process of muscle protein balance, ActRIIB signal pathway stimulates FoxO -dependent transcription to enhance muscle protein catabolism and suppresses Akt / mTOR signaling to inhibit muscle protein synthesis ( Han et al, 2013 ; Hulmi et al, 2013 ; Tian et al, 2018 ; Tian, Lin & Jiao, 2019 ). Although the expression of mTOR was not influenced after dsRNA injection of Es-ActRIIB , the low expression of FoxO was observed, resulting in the slowed down protein catabolism that is needed to accelerate muscle protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

Wang

Zhang

Hou

et al. 2020

PeerJ

View full text Add to dashboard Cite

Activin receptor IIB (ActRIIB) is a serine/threonine-kinase receptor binding with transforming growth factor-β (TGF-β) superfamily ligands to participate in the regulation of muscle mass in vertebrates. However, its structure and function in crustaceans remain unknown. In this study, the ActRIIB gene in Eriocheir sinensis (Es-ActRIIB) was cloned and obtained with a 1,683 bp open reading frame, which contains the characteristic domains of TGF-β type II receptor superfamily, encoding 560 amino acids. The mRNA expression of Es-ActRIIB was the highest in hepatopancreas and the lowest in muscle at each molting stage. After injection of Es-ActRIIB double-stranded RNA during one molting cycle, the RNA interference (RNAi) group showed higher weight gain rate, higher specific growth rate, and lower hepatopancreas index compared with the control group. Meanwhile, the RNAi group displayed a significantly increased content of hydrolytic amino acid in both hepatopancreas and muscle. The RNAi group also displayed slightly higher contents of saturated fatty acid and monounsaturated fatty acid but significantly decreased levels of polyunsaturated fatty acid compared with the control group. After RNAi on Es-ActRIIB, the mRNA expressions of five ActRIIB signaling pathway genes showed that ActRI and forkhead box O (FoxO) were downregulated in hepatopancreas and muscle, but no significant expression differences were found in small mother against decapentaplegic (SMAD) 3, SMAD4 and mammalian target of rapamycin. The mRNA expression s of three lipid metabolism-related genes (carnitine palmitoyltransferase 1β (CPT1β), fatty acid synthase, and fatty acid elongation) were significantly downregulated in both hepatopancreas and muscle with the exception of CPT1β in muscles. These results indicate that ActRIIB is a functionally conservative negative regulator in growth mass, and protein and lipid metabolism could be affected by inhibiting ActRIIB signaling in crustacean.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

Wang

Zhang

Hou

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…ActRI is the interacting protein of ActRIIB binding to myostatin (Hata & Chen, 2016), and its lower expression was observed with interference of Es-ActRIIB in this study; the expressions of downstream transcription factors SMAD3 and SMAD4 were not influenced, the reason probably was that SMADs, as core and versatile cytokines, are active in the TGF-β pathway, including the signal transduction of TGF-β/BMP/activin, not only in myostatin/ActRIIB pathway (Massague, Seoane & Wotton, 2005;Tian et al, 2018b). Moreover, in the process of muscle protein balance, ActRIIB signal pathway stimulates FoxOdependent transcription to enhance muscle protein catabolism and suppresses Akt/mTOR signaling to inhibit muscle protein synthesis (Han et al, 2013;Hulmi et al, 2013;Tian et al, 2018a;Tian et al, 2019a). Although the expression of mTOR was not influenced after dsRNA injection of Es-ActRIIB, the low expression of FoxO was observed, resulting in the slowed down protein catabolism that is needed to accelerate muscle protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis (v0.1)"

2020

View full text Add to dashboard Cite

Activin receptor IIB (ActRIIB) is a serine/threonine-kinase receptor binding with transforming growth factor-β (TGF-β) superfamily ligands to participate in the regulation of muscle mass in vertebrates. However, its structure and function in crustaceans remain unknown. In this study, the ActRIIB gene in Eriocheir sinensis (Es-ActRIIB) was cloned and obtained with a 1683 bp open reading frame, which contains the characteristic domains of TGF-β type II receptor superfamily, encoding 560 amino acids. The mRNA expression of Es-ActRIIB was the highest in hepatopancreas and the lowest in muscle at each molting stage. After injection of Es-ActRIIB double-stranded RNA during one molting cycle, the RNA interference (RNAi) group showed higher weight gain rate, higher specific growth rate, and lower hepatopancreas index compared with the control group. Meanwhile, the RNAi group displayed a significantly increased content of hydrolytic amino acid in both hepatopancreas and muscle. The RNAi group also displayed slightly higher contents of saturated fatty acid and monounsaturated fatty acid but significantly decreased levels of polyunsaturated fatty acid compared with the control group. After RNAi on Es-ActRIIB, the mRNA expressions of five ActRIIB signaling pathway genes showed that ActRI and forkhead box O (FoxO) were downregulated in hepatopancreas and muscle, but no significance expression differences were found in small mother against decapentaplegic (SMAD) 3, SMAD4 and mammalian target of rapamycin. The mRNA expression s of three lipid metabolism-related genes (carnitine palmitoyltransferase 1β (CPT1β), fatty acid synthase, and fatty acid elongation) were significantly downregulated in both hepatopancreas and muscle with the exception of CPT1β in muscles. These results indicate that ActRIIB is a functionally conservative negative regulator in growth mass, and protein and lipid metabolism could be affected by inhibiting ActRIIB signaling in crustacean.

show abstract

“…S6 kinase also plays an important regulatory role in muscle growth during E. sinensis molting process (Tian et al, 2019;Hou et al, 2020;Tian et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…( Yu et al, 2018 ; Chen et al, 2019 ; Wang et al, 2020a ; Liu et al, 2021a ; Zhang et al, 2021 ). Moreover, the role of several regulatory genes such as V-ATPase subunit B (VATB), transforming growth factor-beta type I receptor (TGFBR1) and S6 kinase has also been studied ( Tian et al, 2019 ; Hou et al, 2020 ; Tian et al, 2020 ). The results indicated that V-ATPase subunit B plays essential roles in the cuticle formation process of Eriocheir sinensis .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exploration of the regulatory mechanisms of regeneration, anti-oxidation, anti-aging and the immune response at the post-molt stage of Eriocheir sinensis

Wang

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

Eriocheir sinensis is widely appreciated by the surrounding population due to its culinary delicacy and rich nutrients. The E. sinensis breeding industry is very prosperous and molting is one of the important growth characteristics. Research on the regulation of molting in E. sinensis is still in the initial stages. There is currently no relevant information on the regulatory mechanisms of heart development following molting. Comparative transcriptome analysis was used to study developmental regulation mechanisms in the heart of E. sinensis at the post-molt and inter-molt stages. The results indicated that many regulatory pathways and genes involved in regeneration, anti-oxidation, anti-aging and the immune response were significantly upregulated after molting in E. sinensis. Aside from cardiac development, the differentially expressed genes (DEGs) were relevant to myocardial movement and neuronal signal transduction. DEGs were also related to the regulation of glutathione homeostasis and biological rhythms in regard to anti-oxidation and anti-aging, and to the regulation of immune cell development and the immune response. This study provides a theoretical framework for understanding the regulation of molting in E. sinensis and in other economically important crustaceans.

show abstract

Identification of a S6 kinase transcript in the Chinese mitten crab Eriocheir sinensis and its molting-related expression in muscle tissues

Cited by 4 publications

References 37 publications

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

Peer Review #1 of "Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis (v0.1)"

Exploration of the regulatory mechanisms of regeneration, anti-oxidation, anti-aging and the immune response at the post-molt stage of Eriocheir sinensis

Contact Info

Product

Resources

About