Identification of a Scar Marker Linked to a Shattering Resistance Trait in Sesame

Phumichai, Chalermpol; Matthayatthaworn, Weerachai; Chuenpom, Nipha; Wongkaew, Arunee; Somsaeng, Phakaked; Yodyingyong, Tanapong; Panklang, Pherawich; Jenweerawat, Sujin; Keawsaard, Yaowamarn; Phumichai, Thitaporn; Sreewongchai, Tanee; Kaveeta, R.

doi:10.17557/tjfc.359707

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…Previously, Uzun et al (2003) crossed another non-shattering mutant line (cc3) with the Turkish variety Muganli-57, and a closely linked amplified fragment length polymorphism (AFLP) marker was identified in the resulting segregated population. Sequence-characterized amplified region markers associated with shattering resistance were later developed from F 2 lines derived from a cross between Cplus1, a sesame line with shattering-resistant capsules, and KUAOX25, a line with shattering-susceptible capsules (Phumichai et al, 2017). In the present study, we developed InDel marker related to the shattering trait, and therefore the difference between shattered and non-shattered capsule genotypes can be visualized with basic PCR systems and simple agarose gel electrophoresis.…”

Section: Discussionmentioning

confidence: 98%

“…Although heterotic improvements in performance were obtained for seed yield and yield-related traits in these studies, there was no progress with respect to suitability for mechanized harvesting. Besides conventional breeding techniques, molecular markers have also been developed for the closed capsule mutant trait for use in markerassisted selection (Uzun et al, 2003;Phumichai et al, 2017;Zhang H.Y. et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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A High-Density SNP Genetic Map Construction Using ddRAD-Seq and Mapping of Capsule Shattering Trait in Sesame

Yol

Basak²,

Kizil

et al. 2021

Front. Plant Sci.

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The seed-bearing capsule of sesame shatters at harvest. This wildish trait makes the crop unsuitable for mechanized harvesting and also restricts its commercial potential by limiting the cultivation for countries that have no access to low-cost labor. Therefore, the underlying genetic basis of the capsule shattering trait is highly important in order to develop mechanization-ready varieties for sustainable sesame farming. In the present study, we generated a sesame F2 population derived from a cross between a capsule shattering cultivar (Muganli-57) and a non-shattering mutant (PI 599446), which was used to construct a genetic map based on double-digest restriction-site-associated DNA sequencing. The resulting high-density genetic map contained 782 single-nucleotide polymorphisms (SNPs) and spanned a length of 697.3 cM, with an average marker interval of 0.89 cM. Based on the reference genome, the capsule shattering trait was mapped onto SNP marker S8_5062843 (78.9 cM) near the distal end of LG8 (chromosome 8). In order to reveal genes potentially controlling the shattering trait, the marker region (S8_5062843) was examined, and a candidate gene including six CDSs was identified. Annotation showed that the gene encodes a protein with 440 amino acids, sharing ∼99% homology with transcription repressor KAN1. Compared with the capsule shattering allele, the SNP change and altered splicing in the flanking region of S8_5062843 caused a frameshift mutation in the mRNA, resulting in the loss of function of this gene in the mutant parent and thus in non-shattering capsules and leaf curling. With the use of genomic data, InDel and CAPS markers were developed to differentiate shattering and non-shattering capsule genotypes in marker-assisted selection studies. The obtained results in the study can be beneficial in breeding programs to improve the shattering trait and enhance sesame productivity.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

A High-Density SNP Genetic Map Construction Using ddRAD-Seq and Mapping of Capsule Shattering Trait in Sesame

Yol

Basak²,

Kizil

et al. 2021

Front. Plant Sci.

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“…Seed yield reduction in sesame occurs due to high capsule shattering at harvest and uneven capsule ripening characteristics of the sesame crop ( Abdellatef et al, 2008 ; Yol & Uzun, 2019 ; Teklu et al, 2022 ). The scientific evidence indicates that capsule shattering can lead to up to a 50% yield loss during or after harvesting in sesame ( Phumichai, et al, 2017 ). Besides yield reduction, the absence of noon shattering/indehiscent cultivars suited for mechanical harvesting is another topic for sesame improvement ( Abdellatef et al, 2008 ).…”

Section: Other Genes and Qtlsmentioning

confidence: 99%

Discovering favorable genes, QTLs, and genotypes as a genetic resource for sesame (Sesamum indicum L.) improvement

Kefale

Wang²

2022

Front. Genet.

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Sesame (Sesamum indicum L.) is an ancient diploid oilseed crop with high oil content, quality protein, and antioxidant characteristics that is produced in many countries worldwide. The genes, QTLs, and genetic resources of sesame are utilized by sesame researchers and growers. Researchers have identified the many useful traits of this crop, which are available on different platforms. The genes, genotypes, QTLs, and other genetic diversity data of sesame have been collected and stored in more than nine genomic resources, and five sesame crop marker databases are available online. However, data on phenotypic and genotypic variability, which would contribute to sesame improvements, are limited and not yet accessible. The present study comprehensively reviewed more than 110 original published research papers and scientifically incorporated the results. The candidate genes, genotypes, and QTLs of significantly important traits of sesame were identified. Genetic resources related to grain yield and yield component traits, oil content and quality, drought tolerance, salt tolerance, waterlogging resistance, disease resistance, mineral nutrient, capsule shattering resistance, and other agronomic important traits of sesame were studied. Numerous candidate genotypes, genes, QTLs, and alleles associated with those traits were summarized and discovered. The chromosome regions and linkage groups, maps associated with the best traits, and candidate genes were also included. The variability presented in this paper combined with sesame genetic information will help inform further sesame improvement.

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“…Uzun et al [9] employed the Bulked segregant analysis strategy to map the closed capsule trait in an F 2 population derived from crossing the closedcapsular mutant "cc3" with the Turkish variety "Muganli-57"; a total of 72 closed-capsular linked primers were screened and a tightly linked AFLP tag was identi ed. More recently, Phumichai et al [12] constructed an F 2 population, and 192 AFLP primers were screened, of which 9 polymorphic AFLP bands were identi ed. One AFLP molecular marker, Si-SR-32-19, can differentiate between the genotype of a capsule and a closed capsule.…”

Section: Introductionmentioning

confidence: 99%

Morphology and SSR markers-based genetic diversity analysis of sesame varieties released in China

Wang

Zhou

Tang

et al. 2023

Preprint

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Background Sesame, one of the most important oil crops in the world, is rich in sesamol, sesamin, and other antioxidant substances, and can be consumed as oil, food, and vegetable. It has a high nutritional and economic value. To reveal the genetic diversity of the sesame varieties at the molecular and morphological levels, 25 pairs of simple sequence repeat (SSR) primers and 56 biological traits were applied to 101 Chinese sesame varieties released in recent decades, as well as 62 Chinese landraces and 20 alien accessions. Results The results indicated that a total of 166 SSR bands were detected, with an average of 6.64 per marker; the total alleles were 40.35, with an average of 1.614 per primer pair. Among them, the marker LG141 detected a maximum of 1.85 alleles, and SB393 had a minimum of 1.23 alleles. Shannon’s information index ranged from 0.2732 to 0.6497 with an average value of 0.5292; the polymorphic information index ranged from 0.0859 to 0.6357, with an average value of 0.5254, and the average heterozygosity (He) was 0.3246, suggesting that these sesame accessions have a narrow genetic diversity. The results of the unweighted pair-group method with arithmetic averaging (UPGMA) clustering were consistent with those of the binary principal component analysis, and the 183 sesame lines were categorized into three UPGMA groups. The genetic diversity coefficient of sesame germplasm is generally small, and the genetic relativeness is close. The genetic diversity coefficient of Chinese accessions is not much different from that of foreign resources. Moreover, a DNA fingerprint database of 183 sesame accessions including varieties and germplasm resources was constructed, and a corresponding two-dimensional code was developed. Conclusion The results of this study provide data reference for the breeding and promotion of new sesame varieties in China, the protection of new variety rights, the inquiry and identification of DNA genetic information of varieties, and the development and utilization of germplasm resources domestically and abroad.

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Identification of a Scar Marker Linked to a Shattering Resistance Trait in Sesame

Cited by 5 publications

References 27 publications

A High-Density SNP Genetic Map Construction Using ddRAD-Seq and Mapping of Capsule Shattering Trait in Sesame

A High-Density SNP Genetic Map Construction Using ddRAD-Seq and Mapping of Capsule Shattering Trait in Sesame

Discovering favorable genes, QTLs, and genotypes as a genetic resource for sesame (Sesamum indicum L.) improvement

Morphology and SSR markers-based genetic diversity analysis of sesame varieties released in China

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