Identification of a <scp>NAC</scp> transcription factor, <scp>EPHEMERAL</scp>1, that controls petal senescence in Japanese morning glory

Shibuya, Kazuhiko; Shimizu, Kazuo; Niki, Tomoko; Ichimura, Kazuo

doi:10.1111/tpj.12605

Cited by 60 publications

(62 citation statements)

References 36 publications

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“…The flowers of SR opened earlier than those of SCR (Table 1). In flowering plants, flower longevity is species-specific and is closely linked to reproductive strategy (Shibuya et al, 2014). The newly released 'Sanchuraka' cultivars also showed some slight variation in flower longevity among themselves (data not shown).…”

Section: Discussionmentioning

confidence: 91%

Difference in Flower Longevity and Endogenous Ethylene Production of Portulaca umbraticola Cultivars

2016

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Portulaca umbraticola Kunth, with ephemeral flowers, has become an important summer bedding plant in Japan. A lot of new cultivars have recently been bred with different flowering characteristics, but there is little information about P. umbraticola cultivars. In this study, we investigated the differences in flower longevity, endogenous ethylene production and ethylene sensitivity between a conventional cultivar, 'Single Red' (SR), and a newly released cultivar, 'Sanchuraka Cherry Red' (SCR). The flowers of SR opened and closed earlier than those of SCR and the flower longevity of SCR was significantly longer than that of SR. The effects of pollination, filament wounding and pistil removal on flower longevity were also investigated in both cultivars. Pollination, filament wounding and pistil removal significantly accelerated senescence in both cultivars, but filament wounding was much more significant in accelerating senescence. Endogenous ethylene production from flower opening to closure was significantly higher in SR than in SCR. The peak ethylene production in SR occurred 2 h earlier than that in SCR. Exogenous ethylene treatments of 0.5, 1, and 2 μL·L −1 significantly accelerated the rate of senescence in both SR and SCR. The use of ethylene action inhibitor 1-methylcyclopropene (1-MCP) and ethylene biosynthesis inhibitor aminoethoxyvinylglycine (AVG) significantly improved flower longevity in both cultivars, with the latter being much more effective. The better flower longevity of SCR seems to be related to lower endogenous ethylene production. The senescence of P. umbraticola cultivars seems to be ethylene-dependent.

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Section: Discussionmentioning

confidence: 91%

Difference in Flower Longevity and Endogenous Ethylene Production of Portulaca umbraticola Cultivars

2016

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“…Members of the plant-specific NAM/ATAF/CUC (NAC) transcription factor family are involved in petal senescence in several plant species. In morning glory (Ipomoea nil), the silencing of EPHEMERAL1, a NAC transcription factor, suppresses petal senescence [38]. In rose, two HD-Zip proteins, RhHB1 and RhHB6, regulate petal senescence positively and negatively, respectively [39,40].…”

Section: Transcription Factors and Protein Kinasesmentioning

confidence: 99%

Proteome and Ubiquitome Changes during Rose Petal Senescence

Lü

Fan

et al. 2019

IJMS

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Petal senescence involves numerous programmed changes in biological and biochemical processes. Ubiquitination plays a critical role in protein degradation, a hallmark of organ senescence. Therefore, we investigated changes in the proteome and ubiquitome of senescing rose (Rosa hybrida) petals to better understand their involvement in petal senescence. Of 3859 proteins quantified in senescing petals, 1198 were upregulated, and 726 were downregulated during senescence. We identified 2208 ubiquitinated sites, including 384 with increased ubiquitination in 298 proteins and 1035 with decreased ubiquitination in 674 proteins. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that proteins related to peptidases in proteolysis and autophagy pathways were enriched in the proteome, suggesting that protein degradation and autophagy play important roles in petal senescence. In addition, many transporter proteins accumulated in senescing petals, and several transport processes were enriched in the ubiquitome, indicating that transport of substances is associated with petal senescence and regulated by ubiquitination. Moreover, several components of the brassinosteroid (BR) biosynthesis and signaling pathways were significantly altered at the protein and ubiquitination levels, implying that BR plays an important role in petal senescence. Our data provide a comprehensive view of rose petal senescence at the posttranslational level.

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“…In Japanese morning glory (Ipomoea nil (L.) Roth), a NAC (NAM/ATAF1,2/CUC2) transcription factor, EPHEMERAL1 (EPH1), is a key regulator of ethyleneindependent petal senescence (Shibuya, 2018;Shibuya et al, 2014). As dahlia flowers also show ethyleneindependent petal senescence, an EPH1 homolog may be involved in petal aging in dahlia.…”

Section: Discussionmentioning

confidence: 99%

Breeding for Long Vase Life in Dahlia (Dahlia variabilis) Cut Flowers

Onozaki

Azuma

2019

Hort. J.

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Flower vase life is one of the most important traits for ornamental plants. The vase life of cut dahlia (Dahlia variabilis) flowers is very short, and genetic improvement of this trait is desirable. We started a breeding research program in 2014 to improve the vase life of dahlia flowers using conventional cross-breeding techniques. We found large significant differences in flower vase life among 24 dahlia cultivars: Nine cultivars had long vase life (e.g., 'Syukuhai', 'Rinka', and 'Micchan'); eight had normal vase life (e.g., 'Kamakura', 'Agitate', and 'Benifusya'); and seven had short vase life (e.g., 'Gin-Ei', 'Port Light Pair Beauty', and 'Yumesuiren'). We used 22 cultivars as initial breeding materials, repeatedly crossed them, and selected promising offspring with long vase life for three generations from 2014 to 2018. Two cycles of selection and crossing led to a 1.7-day increase in vase life (population mean) from the first to the third generation, clearly showing that this approach can extend the vase life of dahlia flowers. The mean vase life of 'Kamakura', a leading white dahlia cultivar in Japan, was 5.0-6.2 days in distilled water, 6.0-6.8 days in an isothiazolinic antibacterial agent CMIT/MIT solution (5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3one) and 6.0-7.6 days in a GLA solution (10 g•L −1 glucose, 0.5 ml•L −1 CMIT/MIT, and 50 mg•L −1 aluminum sulfate), whereas in six finally selected lines it was 6.2-12.0 days in distilled water, 6.6-10.2 days in CMIT/MIT solution, and 9.4-13.6 days in GLA solution (1.4-2.1 times that in 'Kamakura'). In particular, the selected second-generation line 606-46 showed a stably longer vase life than 'Kamakura'. 'Micchan', which has a long vase life, was a common progenitor used for breeding of parental lines in cross combinations with long vase life in the second generation and all cross combinations in the third generation. The final six selected lines with long vase life were all progeny of 'Micchan'. Our results strongly suggest that 'Micchan' has genes related to long flower vase life, and that the trait is heritable.

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Identification of a NAC transcription factor, EPHEMERAL1, that controls petal senescence in Japanese morning glory

Cited by 60 publications

References 36 publications

Difference in Flower Longevity and Endogenous Ethylene Production of <i>Portulaca umbraticola</i> Cultivars

Difference in Flower Longevity and Endogenous Ethylene Production of <i>Portulaca umbraticola</i> Cultivars

Proteome and Ubiquitome Changes during Rose Petal Senescence

Breeding for Long Vase Life in Dahlia (<i>Dahlia variabilis</i>) Cut Flowers

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