Identification of a <scp>UDP</scp>‐glucosyltransferase conferring deoxynivalenol resistance in <i>Aegilops tauschii</i> and wheat

Kirana, Rizky Pasthika; Gaurav, Kumar; Arora, Sanu; Wiesenberger, Gerlinde; Doppler, Maria; Michel, Sebastian; Zimmerl, Simone; Matić, Magdalena; Eze, Chinedu Emmanuel; Kumar, Mukesh; Topuz, Ajla; Lemmens, Marc; Schuhmacher, Rainer; Adam, Gerhard; Wulff, Brande B. H.; Buerstmayr, Hermann; Steiner, Barbara

doi:10.1111/pbi.13928

Cited by 7 publications

(3 citation statements)

References 78 publications

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“…Genetic approaches demonstrated this in different plants and tissues for cereal UGTs [ 1 ] and in wheat for a GST that underpins the DON and FHB resistance QTL Fhb7 [ 31 ]. A mutation in a UGT from Aegilops tauschii , which is the diploid progenitor of the wheat D subgenome, affected the ability of the plant to convert DON to its less toxic derivative, DON-3-glucoside [ 41 ]. The authors speculated that the truncated version of this gene present in hexaploid wheat may increase toxin and FHB susceptibility.…”

Section: Plant Resistance To Trichothecenes and Fumonisinsmentioning

confidence: 99%

Trichothecenes and Fumonisins: Key Players in Fusarium–Cereal Ecosystem Interactions

Perochon,

Doohan

2024

Toxins

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Fusarium fungi produce a diverse array of mycotoxic metabolites during the pathogenesis of cereals. Some, such as the trichothecenes and fumonisins, are phytotoxic, acting as non-proteinaceous effectors that facilitate disease development in cereals. Over the last few decades, we have gained some depth of understanding as to how trichothecenes and fumonisins interact with plant cells and how plants deploy mycotoxin detoxification and resistance strategies to defend themselves against the producer fungi. The cereal-mycotoxin interaction is part of a co-evolutionary dance between Fusarium and cereals, as evidenced by a trichothecene-responsive, taxonomically restricted, cereal gene competing with a fungal effector protein and enhancing tolerance to the trichothecene and resistance to DON-producing F. graminearum. But the binary fungal–plant interaction is part of a bigger ecosystem wherein other microbes and insects have been shown to interact with fungal mycotoxins, directly or indirectly through host plants. We are only beginning to unravel the extent to which trichothecenes, fumonisins and other mycotoxins play a role in fungal-ecosystem interactions. We now have tools to determine how, when and where mycotoxins impact and are impacted by the microbiome and microfauna. As more mycotoxins are described, research into their individual and synergistic toxicity and their interactions with the crop ecosystem will give insights into how we can holistically breed for and cultivate healthy crops.

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Section: Plant Resistance To Trichothecenes and Fumonisinsmentioning

confidence: 99%

Trichothecenes and Fumonisins: Key Players in Fusarium–Cereal Ecosystem Interactions

Perochon,

Doohan

2024

Toxins

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“…DON detoxification by glycosylation is a protective mechanism commonly observed in host plants. A survey of the current literature highlights examples of DON glycosylation across multiple crop hosts that include maize, barley, rice, oats, wheat, Aegilops, and Arabidopsis [9][10][11][12][13][14][15]. In a number of these cases, specific enzymes belonging to UDP-Glycosyl Transferases (UDP-GTases) Family 1 class of enzymes were implicated in the production of DON-3-O-glucosides (D3G) and other glycosylated analogs.…”

Section: Introductionmentioning

confidence: 99%

“…In a number of these cases, specific enzymes belonging to UDP-Glycosyl Transferases (UDP-GTases) Family 1 class of enzymes were implicated in the production of DON-3-O-glucosides (D3G) and other glycosylated analogs. Some of the UDP-GTases are encoded by the genes AET5Gv20385300 in Aegilops, AVESA.00010b.r2.6AG1068650.1 and AVESA.00010b.r2.6AG1068570.1 in oat [13,14], Os04g0206600 (Os70) in rice [16], and include the proteins HvUGT13248 in barley [9,12,17] and UGT73C5 in Arabidopsis [11]. Of the fore mentioned enzymes, the rice enzyme Os70 is the most thoroughly characterized, including functional studies based on a 3-dimensional Os70 co-crystal structure with a fluorinated uridine-diphosphate-glucose analogue and bound trichothecenes (PDB ID 5TMD) [16,18].…”

Section: Introductionmentioning

confidence: 99%

Multiple Clonostachys rosea UDP-Glycosyltransferases Contribute to the Production of 15-Acetyl-Deoxynivalenol-3-O-Glycoside When Confronted with Fusarium graminearum

Robinson

St-Jacques

Shields

et al. 2023

JoF

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Mycotoxins, derived from toxigenic fungi such as Fusarium, Aspergillus, and Penicillium species have impacted the human food chain for thousands of years. Deoxynivalenol (DON), is a tetracyclic sesquiterpenoid type B trichothecene mycotoxin predominantly produced by F. culmorum and F. graminearum during the infection of corn, wheat, oats, barley, and rice. Glycosylation of DON is a protective detoxification mechanism employed by plants. More recently, DON glycosylating activity has also been detected in fungal microparasitic (biocontrol) fungal organisms. Here we follow up on the reported conversion of 15-acetyl-DON (15-ADON) into 15-ADON-3-O-glycoside (15-ADON-3G) in Clonostachys rosea. Based on the hypothesis that the reaction is likely being carried out by a uridine diphosphate glycosyl transferase (UDP-GTase), we applied a protein structural comparison strategy, leveraging the availability of the crystal structure of rice Os70 to identify a subset of potential C. rosea UDP-GTases that might have activity against 15-ADON. Using CRISPR/Cas9 technology, we knocked out several of the selected UDP-GTases in the C. rosea strain ACM941. Evaluation of the impact of knockouts on the production of 15-ADON-3G in confrontation assays with F. graminearum revealed multiple UDP-GTase enzymes, each contributing partial activities. The relationship between these positive hits and other UDP-GTases in fungal and plant species is discussed.

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The wild and the valuable: The goatgrass pangenome advances wheat improvement

Mao

2024

The Crop Journal

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Identification of a UDP‐glucosyltransferase conferring deoxynivalenol resistance in Aegilops tauschii and wheat

Abstract: B. (2022). Identification of a UDP -glucosyltransferase conferring deoxynivalenol resistance in Aegilops tauschii and wheat. Plant Biotechnology Journal. Portico.

Cited by 7 publications

References 78 publications

Trichothecenes and Fumonisins: Key Players in Fusarium–Cereal Ecosystem Interactions

Trichothecenes and Fumonisins: Key Players in Fusarium–Cereal Ecosystem Interactions

Multiple Clonostachys rosea UDP-Glycosyltransferases Contribute to the Production of 15-Acetyl-Deoxynivalenol-3-O-Glycoside When Confronted with Fusarium graminearum

The wild and the valuable: The goatgrass pangenome advances wheat improvement

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