Identification of a secreted superoxide dismutase in Mycobacterium avium ssp. paratuberculosis

Liu, X

doi:10.1016/s0378-1097(01)00325-1

Cited by 21 publications

(32 citation statements)

References 21 publications

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“…However, M. avium subsp. paratuberculosis sod, with a manganese-binding cofactor, was found to be secreted into the culture supernatant without a signal sequence of its own and was translocated by the specific export machinery of mycobacteria (25). The copper-and zinc-cofactored superoxide dismutase encoded by the sodC gene from M. avium subsp.…”

Section: Figmentioning

confidence: 99%

In Silico Identification of Epitopes in Mycobacterium avium subsp. paratuberculosis Proteins That Were Upregulated under Stress Conditions

Gurung

Purdie

Begg

et al. 2012

Clin Vaccine Immunol

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ABSTRACTJohne's disease in ruminants is caused byMycobacterium aviumsubsp.paratuberculosis. Diagnosis ofM. aviumsubsp.paratuberculosisinfection is difficult, especially in the early stages. To date, ideal antigen candidates are not available for efficient immunization or immunodiagnosis. This study reports thein silicoselection and subsequent analysis of epitopes ofM. aviumsubsp.paratuberculosisproteins that were found to be upregulated under stress conditions as a means to identify immunogenic candidate proteins. Previous studies have reported differential regulation of proteins whenM. aviumsubsp.paratuberculosisis exposed to stressors which induce a response similar to dormancy. Dormancy may be involved in evading host defense mechanisms, and the host may also mount an immune response against these proteins. Twenty-fiveM. aviumsubsp.paratuberculosisproteins that were previously identified as being upregulated underin vitrostress conditions were analyzed for B and T cell epitopes by use of the prediction tools at the Immune Epitope Database and Analysis Resource. Major histocompatibility complex class I T cell epitopes were predicted using an artificial neural network method, and class II T cell epitopes were predicted using the consensus method. Conformational B cell epitopes were predicted from the relevant three-dimensional structure template for each protein. Based on the greatest number of predicted epitopes, eight proteins (MAP2698c [encoded bydesA2], MAP2312c [encoded byfadE19], MAP3651c [encoded byfadE3_2], MAP2872c [encoded byfabG5_2], MAP3523c [encoded byoxcA], MAP0187c [encoded bysodA], and the hypothetical proteins MAP3567 and MAP1168c) were identified as potential candidates for study of antibody- and cell-mediated immune responses within infected hosts.

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Section: Figmentioning

confidence: 99%

In Silico Identification of Epitopes in Mycobacterium avium subsp. paratuberculosis Proteins That Were Upregulated under Stress Conditions

Gurung

Purdie

Begg

et al. 2012

Clin Vaccine Immunol

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“…16S rDNA sequencing has demonstrated low variability when compared with the Lipav2 assay (Lebrun et al, 2005). M. avium serotype 2 SalI-EcoRI DT6 and SalI-BamHI DT1 fragment probing (Thierry et al, 1993) and sequencing of the sodA (Liu et al, 2001), dnaJ (Morita et al, 2004), gyrB (Kasai et al, 2000), recA (Blackwood et al, 2000), the 32 kDa protein gene (Soini et al, 1996) or a combination thereof (Devulder et al, 2005) have been further used to identify MAC species. These studies, however, did not include all of the four MAC species and used restricted panels of reference strains, thus limiting the significance of the data.…”

Section: Introductionmentioning

confidence: 99%

rpoB sequence-based identification of Mycobacterium avium complex species

et al. 2008

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The Mycobacterium avium complex (MAC) comprises slowly growing mycobacteria responsible for opportunistic infections and zoonoses. The ability to speciate MAC isolates in the clinical microbiology laboratory is critical for determining the organism implicated in clinical disease and for epidemiological investigation of the source of infection. Investigation of a 711 bp variable fragment of rpoB flanked by the Myco-F/Myco-R primers found a 0.7-5.1 % divergence among MAC reference strains, with Mycobacterium chimaera and Mycobacterium intracellulare being the most closely related. Using a 0.7 % divergence cut-off, 83 % of 100 clinical isolates, which had been previously identified by phenotypic characteristics and 16S-23S rDNA intergenic spacer (ITS) probing, were identified as M. avium, 8 % as M. intracellulare and 2 % as M. chimaera. The uniqueness of seven isolates, exhibiting ,99.3 % rpoB sequence similarity with MAC reference strains, was confirmed by 16S rDNA, ITS and hsp65 sequencing and phylogenetic analyses. Partial rpoB gene sequencing using the Myco-F/Myco-R primers permits one-step identification of MAC isolates at the species level and the detection of potentially novel MAC species.

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“…paratuberculosis. Among these are the heat shock proteins GroES (9) and GroEL (14), 2 alkyl hydroperoxide reductases (25), a serine protease (6), superoxide dismutase (21), and 11 other proteins of unknown function that are named on the basis of their sizes in kilodaltons (3, 10, 13, 15, 16, 23, 24, 26-28, 33, 37). Some of these antigens appear to show promise, but none has been incorporated as a routine diagnostic tool according to the published literature and available commercial tests.…”

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confidence: 99%

Expression and Immunogenicity of Proteins Encoded by Sequences Specific toMycobacterium aviumsubsp.paratuberculosis

et al. 2004

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The development of immunoassays specific for the diagnosis of Johne's disease in cattle requires antigens specific to Mycobacterium avium subsp. paratuberculosis. However, because of genetic similarity to other mycobacteria comprising the M. avium complex, no such antigens have been found. Through a comparative genomics approach, 21 potential coding sequences of M. avium subsp. paratuberculosis that are not represented in any other mycobacterial species tested (n ‫؍‬ 9) were previously identified (

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Identification of a secreted superoxide dismutase in Mycobacterium avium ssp. paratuberculosis

Cited by 21 publications

References 21 publications

In Silico Identification of Epitopes in Mycobacterium avium subsp. paratuberculosis Proteins That Were Upregulated under Stress Conditions

In Silico Identification of Epitopes in Mycobacterium avium subsp. paratuberculosis Proteins That Were Upregulated under Stress Conditions

rpoB sequence-based identification of Mycobacterium avium complex species

Expression and Immunogenicity of Proteins Encoded by Sequences Specific toMycobacterium aviumsubsp.paratuberculosis

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