Identification of a specific marker for hepatitis C virus infection using capillary zone electrophoresis

Attallah, Abdelfattah M.; Malak, Camelia A. Abdel; El-Ghawalby, Nabih; Shehatta, Ahmed S.; Abdel-Raouf, Mohamed; Shiha, Gamal

doi:10.1016/j.cccn.2004.03.024

Cited by 11 publications

(8 citation statements)

References 29 publications

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“…[16] The main A. M. Attallah et al 206 treatment goal in patients with chronic HCV infection is the prevention of progressive hepatic fibrosis by early diagnosis. [17] There is a clinical need for noninvasive measurement of liver fibrosis both to diagnose significant liver fibrosis and to monitor the effects of therapy on fibrogenesis and fibrolysis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Serum Procollagen Aminoterminal Propeptide III, Laminin, and Hydroxyproline as Predictors of Severe Fibrosis in Patients with Chronic Hepatitis C

Attallah¹,

Toson²,

Shiha³

et al. 2007

Journal of Immunoassay and Immunochemistry

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In an attempt to identify biochemical analytes that could enhance the discrimination between the patients with severe liver fibrosis (F3-F4) and mild fibrosis (F1-F2) based on absolute values of biochemical markers, we measured 12 analytes, including procollagen III aminoterminal propeptide (PIIINP), laminin, proline, hydroxylproline, glycine, AST, ALT, alkaline phosphatase, albumin, total bilirubin, total protein, and prothrombin time in 252 individuals with chronic hepatitis C infection (CHC). PIIINP and laminin were determined by radio-immunoassay; the degraded amino acids were determined using high performance liquid chromatography. Statistical analyses were performed by logistic regression, and receiver operating characteristic (ROC) curves. The best linear combination of blood markers was selected by multivariate discriminant analysis (MDA) for construction of the fibrosis discriminant score (FDS). FDS, an index of five markers (PIIINP, laminin, hydroxyproline, prothrombin activity, and AST/ALT) correctly classified 82% of the patients with severe liver fibrosis at a discriminant cut-off score=-0.5 (i.e., less than -0.5 indicated severe liver fibrosis and greater than -0.5 indicated mild liver fibrosis with sensitivity (76%) and specificity (89%). This result was reproduced in a validation study with no significant difference. In conclusion, FDS is useful for identifying severe liver fibrosis in patients with CHC.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Serum Procollagen Aminoterminal Propeptide III, Laminin, and Hydroxyproline as Predictors of Severe Fibrosis in Patients with Chronic Hepatitis C

Attallah¹,

Toson²,

Shiha³

et al. 2007

Journal of Immunoassay and Immunochemistry

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“…[1] The exact diagnosis is key for the understanding of the natural course and prognosis of liver disease. [2,3] Liver fibrosis progression occurs in two-thirds of patients with initially mild chronic hepatitis C within 5 -10 years and advanced fibrosis develops in one-third of those with no fibrosis. [4] The assessment of the presence and severity of liver fibrosis is of paramount importance in determining treatment strategies, response to treatment, prognosis, and the potential risk for complications in patients with chronic liver disease.…”

Section: Introductionmentioning

confidence: 99%

Immunodetection of Collagen Types I, II, III, and IV for Differentiation of Liver Fibrosis Stages in Patients with Chronic HCV

Attallah

Mosa

Omran

et al. 2007

Journal of Immunoassay and Immunochemistry

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The current study is aimed at evaluating serum collagens and other serum biochemical markers as useful, non-invasive markers of hepatic fibrosis associated with chronic hepatitis C virus (HCV). Collagen types I, II, III, and IV were detected in serum using ELISA and Western blot techniques. The ELISA levels of collagen I, II, III, and IV increased significantly with the progression of fibrosis staging. Based on receiver-operating characteristic (ROC) curve analysis, the collagen type III (70 kDa) and type IV (200 kDa) were more useful than other serum bio-markers for differentiating severe fibrosis from mild fibrosis. Multivariate discriminant analysis (MDA) selected a fibrosis discriminant score (FDS) = [2.345 + Collagen III (microg/mL) x 1.923 + Collagen IV (microg/mL) x 1.544 + ALT (U/mL) x 0.005] - [albumin(g/L) x 0.046]. The FDS correctly classified 87% of the severe fibrosis patients at a cut-off score = 2.2 (i.e., more than 2.2 indicated severe fibrotic liver and less than 2.2 indicated mild fibrotic liver) with specificity of 97%. In a validation study, the FDS was applied to the second cohort of patients and the results were reproduced without significant difference. In conclusion, the developed four-parameter based FDS is useful for identifying severe liver fibrosis in patients with chronic HCV infection.

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“…40 In the same year, Shiha and co-workers identified hepatitis C virus in human urine samples. 41 The characteristic peak at migration time 2.72 min was collected by using CZE and was subjected to nested PCR. The amplified product of the fractionally collected peak was detected by agarose gel electrophoresis.…”

Section: Observing Bacterial Migration Behaviormentioning

confidence: 99%

Electrokinetic Detection and Characterization of Intact Microorganisms

Kłodzińska

Buszewski

2008

Anal. Chem.

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Achievements in bacteria analysis with electromigration techniques may improve medical diagnoses, detection of food contamination, and sterility testing. (To listen to a podcast about this feature, please go to the Analytical Chemistry website at pubs.acs.org/ac.)An important research problem in the modern clinical laboratory is the discovery of new biomarkers, the presence of which can indicate a particular disease state; for example, the presence of an antibody may indicate an infection. The complex nature of biological samples and the low concentrations of analytes demand a system with high sensitivity and efficiency. Capillary zone electrophoresis (CZE) is such a system, and it promises to rival HPLC when applied to the separation of charged and neutral species in biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis, environmental science, medical diagnosis, and clinical settings. 1,2 One area in which CZE may have an inherent advantage over HPLC is the analysis of small particles of colloidal sizes, such as cells.The rapid and sensitive determination of pathogenic microorganisms is extremely important in biotechnology, quality control of probiotics, and bacterial analysis, but it is critical for early and effective disease management and antimicrobial therapy, especially for infant patients, whose immunological systems are not fully developed. Some microbes are active ingredients in health products, medicines, and supplements. On the other hand, some pathogenic microorganisms can be considered possible biological warfare agents and constitute a significant cause of death in many countries (Bacillus anthracis, Rickettsia rickettsii, and Salmonella typhi). 2 Biological weapons include bacteria, viruses, fungi, and toxins found in nature that can be used to kill or injure people. Pathogenic bacteria are the most common causes of food-and water-borne illnesses.However, conventional microbiological techniques of bacterial identification, culture, and isolation by biochemical and serological assay are time-consuming and labor-intensive. Therefore, fast and selective analytical techniques that more rapidly and effectively determine microbial contamination or infection are required. In this article, we summarize the most important achievements in microbial separation by electromigration techniques and describe the different applications. ORIGIN OF MICROBIAL CHARGESmall viruses have diameters in the range of several tens of nanometers. Bacteria are generally larger, some by a factor of 100, and the increased size leads to increased complexity. Viruses exist mainly in helical and/or icosahedral forms, whereas bacteria can adopt an enormous variety of shapes and sizes, both among and within species. These physiological differences can make the characterization and identification of bacterial cells by electromigration techniques more difficult (Figure 1a). The microbial surface charge originates from the ionization of surface molecules and the adsorption of ions from solution. Bacterial cell wal...

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Identification of a specific marker for hepatitis C virus infection using capillary zone electrophoresis

Cited by 11 publications

References 29 publications

Evaluation of Serum Procollagen Aminoterminal Propeptide III, Laminin, and Hydroxyproline as Predictors of Severe Fibrosis in Patients with Chronic Hepatitis C

Evaluation of Serum Procollagen Aminoterminal Propeptide III, Laminin, and Hydroxyproline as Predictors of Severe Fibrosis in Patients with Chronic Hepatitis C

Immunodetection of Collagen Types I, II, III, and IV for Differentiation of Liver Fibrosis Stages in Patients with Chronic HCV

Electrokinetic Detection and Characterization of Intact Microorganisms

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