Identification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsis

Li, Bo; Byrt, Caitlin S.; Qiu, Jiaen; Baumann, Ute; Hrmová, Mária; Evrard, Aurélie; Johnson, Alexander; Birnbaum, Kenneth D.; Mayo, Gwenda M.; Jha, Deepa; Henderson, Sam W.; Tester, Mark; Gilliham, Matthew; Roy, Stuart J.

doi:10.1104/pp.15.01163

Cited by 92 publications

(104 citation statements)

References 107 publications

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“…To express NPF2.5 in X. laevis oocytes, a pGEMHE-DEST expression vector (Li et al, 2016) was used. cRNA was prepared using a mMESSAGE mMACHINE kit (Ambion, CA) following the manufacturer's manual.…”

Section: Methodsmentioning

confidence: 99%

“…However, the finding that AtCCC and Vitis vinifera CCC (VviCCC) are localized to the Golgi and trans -Golgi network (Henderson et al, 2015), and that Orysa sativa CCC (OsCCC) is involved in osmoregulation and tissue growth suggests that the role of CCC in long distance Cl − transport is likely to be indirect. More recently, AtNPF2.4 (Li et al, 2016) and AtSLAH1 ( Slow Anion Channel Associated 1 Homolog1 ) (Cubero-Font et al, 2016; Qiu et al, 2016) have been identified as genes encoding plasma membrane-localized anion transporters in the root stele that facilitate transfer of Cl − into the root xylem. As one member of the NRT1/PTRs in Arabidopsis, NPF2.4 belongs to the NAXT (for Nitrate Excretion Transporter) subfamily (a seven-gene clade) that was named after NAXT1 ( NPF2.7 ), a plasma membrane transporter involved in the efflux of

{NO}_{3}^{-}

from the root surface (Segonzac et al, 2007; Tsay et al, 2007; Léran et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Another NAXT family member, NPF2.3, was identified following functional characterization as a transporter that was more selective for

{NO}_{3}^{-}

than for Cl − and was important for root-to-shoot transfer of

{NO}_{3}^{-}

(Taochy et al, 2015). NPF2.3 and NPF2.4 were both shown to be regulated by NaCl, indicating the possible involvement of the NAXT subfamily in plant salinity responses (Taochy et al, 2015; Li et al, 2016). The other four NAXT genes, NPF2.1, NPF2.2, NPF2.5 , and NPF2.6 , are yet to be functionally characterized.…”

Section: Introductionmentioning

confidence: 99%

“…NPF2.5 was revealed by a comparative microarray study to be preferentially expressed in the root (Tsay et al, 2007). On the basis of having the smallest phylogenetic distance in protein sequence to the Cl − -efflux transporter NPF2.4 (Li et al, 2016), it was hypothesized that NPF2.5 may also function as a Cl − transporter in the root.…”

Section: Introductionmentioning

confidence: 99%

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AtNPF2.5 Modulates Chloride (Cl−) Efflux from Roots of Arabidopsis thaliana

Qiu

Jayakannan

et al. 2017

Front. Plant Sci.

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The accumulation of high concentrations of chloride (Cl−) in leaves can adversely affect plant growth. When comparing different varieties of the same Cl− sensitive plant species those that exclude relatively more Cl− from their shoots tend to perform better under saline conditions; however, the molecular mechanisms involved in maintaining low shoot Cl− remain largely undefined. Recently, it was shown that the NRT1/PTR Family 2.4 protein (NPF2.4) loads Cl− into the root xylem, which affects the accumulation of Cl− in Arabidopsis shoots. Here we characterize NPF2.5, which is the closest homolog to NPF2.4 sharing 83.2% identity at the amino acid level. NPF2.5 is predominantly expressed in root cortical cells and its transcription is induced by salt. Functional characterisation of NPF2.5 via its heterologous expression in yeast (Saccharomyces cerevisiae) and Xenopus laevis oocytes indicated that NPF2.5 is likely to encode a Cl− permeable transporter. Arabidopsis npf2.5 T-DNA knockout mutant plants exhibited a significantly lower Cl− efflux from roots, and a greater Cl− accumulation in shoots compared to salt-treated Col-0 wild-type plants. At the same time, NO3− content in the shoot remained unaffected. Accumulation of Cl− in the shoot increased following (1) amiRNA-induced knockdown of NPF2.5 transcript abundance in the root, and (2) constitutive over-expression of NPF2.5. We suggest that both these findings are consistent with a role for NPF2.5 in modulating Cl− transport. Based on these results, we propose that NPF2.5 functions as a pathway for Cl− efflux from the root, contributing to exclusion of Cl− from the shoot of Arabidopsis.

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Section: Methodsmentioning

confidence: 99%

{NO}_{3}^{-}

from the root surface (Segonzac et al, 2007; Tsay et al, 2007; Léran et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Another NAXT family member, NPF2.3, was identified following functional characterization as a transporter that was more selective for

{NO}_{3}^{-}

than for Cl − and was important for root-to-shoot transfer of

{NO}_{3}^{-}

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

AtNPF2.5 Modulates Chloride (Cl−) Efflux from Roots of Arabidopsis thaliana

Qiu

Jayakannan

et al. 2017

Front. Plant Sci.

Self Cite

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show abstract

“…On the other hand, chloride (Cl), together with potassium, has a particular function to stabilize the osmotic potential and turgor pressure (White and Broadley, 2001;Marschner, 2012). Recently, a plasma membrane-localized Nitrate/ Peptide Transporter, NPF2.4, was reported to be involved in the long-distance transport of Cl 2 in Arabidopsis (Arabidopsis thaliana; Li et al, 2016). However, the molecular mechanism for Cl 2 transport in plants is still poorly understood.…”

mentioning

confidence: 99%