Identification of a streptomycin resistance gene and a partial Tn3 transposon coding for a beta-lactamase in a periodontal strain of Eikenella corrodens

Lacroix, Jean-Michel; Walker, Clay

doi:10.1128/aac.36.4.740

Cited by 16 publications

(5 citation statements)

References 22 publications

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“…Although our patients were successfully treated with penicillin, the MICs of penicillin were higher than those in previous reports [22,23]. Strains of b-lactamase-producing Eikenella corrodens have been isolated previously [26,27], but in our study, none of the 23 preserved isolates produced b-lactamase. It is important to monitor the changes in susceptibility of this organism [21].…”

Section: Discussioncontrasting

confidence: 71%

Clinical Features of Patients with Invasive Eikenella corrodens Infections and Microbiological Characteristics of the Causative Isolates

Sheng

Hsueh

Hung

et al. 2001

EJCMID

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Clinical features of 43 cases of invasive Eikenella corrodens infections diagnosed at National Taiwan University Hospital during a 6-year period (1993-1998) were analyzed. The clinical syndromes included head and neck infection (56%), pulmonary infection (23%), intra-abdominal infection (14%), cutaneous infection (5%), skeletal infection (2%), endocarditis (2%), and pelvic abscess (2%). Nearly two-thirds of the patients (63%) had pre-existing diseases. Malignancy (35%), especially of the head and neck, was the most common underlying illness. More than half of the patients (56%) had associated factors predisposing to invasive Eikenella corrodens infection. Polymicrobial infections occurred in 28 (65%) patients, with two-thirds of the concurrent isolates being streptococci (66%). Five cases were fatal, with four deaths directly attributable to invasive Eikenella corrodens infection. Antimicrobial susceptibility testing and molecular typing were performed on 23 preserved Eikenella corrodens isolates. Antimicrobial susceptibility testing showed that Eikenella corrodens isolates were susceptible to penicillin, amoxicillin, cefoxitin, cefotaxime, cefepime, ciprofloxacin, and imipenem. The isolates were resistant to clindamycin, metronidazole, cephalothin, and cefuroxime. None of the 23 isolates produced beta-lactamase. Random amplified polymorphic DNA patterns of the 23 isolates were different, suggesting that different clones of Eikenella corrodens caused these infections.

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Section: Discussioncontrasting

confidence: 71%

Clinical Features of Patients with Invasive Eikenella corrodens Infections and Microbiological Characteristics of the Causative Isolates

Sheng

Hsueh

Hung

et al. 2001

EJCMID

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“…The most common of these is the TEM‐type enzyme originally isolated from ampicillin‐resistant Escherichia coli [11]. Lacroix and Walker [12] found the TEM − 1 β‐lactamase gene in association with a streptomycin resistance gene in an Eikenella corrodens strain isolated from the periodontal pocket. The sequence of this β‐lactamase gene had only one nucleotide difference with the β‐lactamase gene carried on transposon Tn3.…”

Section: Introductionmentioning

confidence: 99%

Detection and characterization of Î²-lactamase genes in subgingival bacteria from patients with refractory periodontitis

Handal

Olsen

Walker

et al. 2005

FEMS Microbiology Letters

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Fifty-three beta-lactamase-producing strains of oral bacteria isolated from patients with refractory periodontitis in Norway and USA were screened for the presence of the bla(TEM), bla(SHV), bla(OXA), bla(ampC), bla(cfxA), and bla(cepA/cblA) genes by the polymerase chain reaction (PCR). The PCR products were characterized by direct sequencing of the amplified DNA. Thirty-four of the 53 enzyme-producing strains (64%) were positive in one of the PCR assays. All beta-lactamase-producing Prevotella and Capnocytophaga spp. were CfxA positive. TEM-type beta-lactamases were identified in one strain each of Escherichia coli and Neisseria sp., and one strain of Citrobacter freundii possessed an AmpC-type beta-lactamase. Screening for gene cassettes and genes known to be associated with integrons did not reveal the presence of integrons in these oral bacteria. Sequence analyses showed that most CfxA positive Prevotella and Capnocytophaga isolates from patients with refractory periodontitis harboured variants of the CfxA2 and CfxA3 enzyme. The present study also showed that many different genetic determinants of beta-lactamase production are found in bacteria isolated from refractory periodontitis, many of which remain to be characterized.

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“…The results of this study support initial observations that beta-lactamase production is becoming increasingly common in Capnocytophaga spp., as in other oral bacteria (3,12,17), but the location of the gene(s) coding for these beta-lactamases is not yet determined. Most investigators report that Capnocytophaga remains susceptible to imipenem and beta-lactamase inhibitor combinations.…”

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confidence: 99%

In Vitro Susceptibilities of Capnocytophaga Isolates to β-Lactam Antibiotics and β-Lactamase Inhibitors

Jolivet‐Gougeon¹,

Buffet²,

Dupuy³

et al. 2000

Antimicrob Agents Chemother

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The susceptibilities of 43 pharyngeal isolates of Capnocytophaga to beta-lactam antibiotics, alone or in combination with beta-lactamase inhibitors, were tested by an agar dilution method. The 34 beta-lactamasepositive strains were highly resistant to beta-lactams, but the intrinsic activities of clavulanate, tazobactam, and sulbactam against Capnocytophaga, even beta-lactamase producers, indicates that these beta-lactamase inhibitors could be used for empirical treatment of neutropenic patients with oral sources of infection.The genus Capnocytophaga is composed of a group of capnophilic, gram-negative fusiform bacteria that are part of the normal oral flora in humans and animals. Capnocytophaga species have been identified as the cause of a variety of infections in immunocompetent hosts (18). In immunocompromised and neutropenic patients, Capnocytophaga spp. have been isolated more frequently from patients with bloodstream infections, including bacteremia (2, 5, 9), and patients with endocarditis (4) with severe chemotherapy-induced ulcerations (8). Variations in the prevalence, number, and proportion of Capnocytophaga spp. have been shown to occur in the dental plaque of pediatric cancer patients undergoing a course of immunosuppressive chemotherapy (22). In general, many antibiotics, including penicillins, clindamycin, macrolides, and quinolones, are effective in treating Capnocytophaga infections (6,10,11,21). However, strains that produce beta-lactamases and that cause septicemia have recently been described (1,7,9,19). These beta-lactamase-producing strains increase the risk of infection in neutropenic patients, especially during chemotherapy. The aim of this study was to determine the susceptibilities of 43 Capnocytophaga strains isolated from neutropenic pediatric patients to beta-lactams and beta-lactamase inhibitors.Forty-three Capnocytophaga strains were isolated by swabbing the throats of pediatric cancer patients undergoing a course of chemotherapy in the Department of Pediatric Oncology at Centre Hospitalier Universitaire Sud (Rennes, France). Two reference strains (Escherichia coli CIP 7624 and Staphylococcus aureus CIP 7625) were also included in the study. Throat samples were collected with sterile swabs, which were immediately taken to the Department of Microbiology, dispersed in sterile distilled water, and inoculated onto TBBP agar (4% Trypticase soy agar supplemented with 5% sheep blood, 0.1% yeast extract [AES Laboratory, Combourg, France], 100 g of polymyxin per ml, 50 g of bacitracin [Sigma] per ml) (15). The agar plates were incubated in a 10% CO 2 atmosphere for 5 days at 37°C. The isolates were identified on the basis of colony morphology, Gram staining, negative catalase and oxidase reactions, and API ZYM profiles (BioMérieux, Marcy l'Etoile, France) (13, 23).All isolates were tested for beta-lactamase production by a chromogenic cephalosporin nitrocefin method (Cefinase; BBL Microbiology Systems, Cockeysville, Md.) by the recommended procedure (Becton Dickinson). The test results we...

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Identification of a streptomycin resistance gene and a partial Tn3 transposon coding for a beta-lactamase in a periodontal strain of Eikenella corrodens

Cited by 16 publications

References 22 publications

Clinical Features of Patients with Invasive Eikenella corrodens Infections and Microbiological Characteristics of the Causative Isolates

Clinical Features of Patients with Invasive Eikenella corrodens Infections and Microbiological Characteristics of the Causative Isolates

Detection and characterization of Î²-lactamase genes in subgingival bacteria from patients with refractory periodontitis

In Vitro Susceptibilities of Capnocytophaga Isolates to β-Lactam Antibiotics and β-Lactamase Inhibitors

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