Identification of a Tall Waterhemp (<i>Amaranthus tuberculatus</i>) Biotype Resistant to HPPD-Inhibiting Herbicides, Atrazine, and Thifensulfuron in Iowa

McMullan, Patrick M.; Green, Jerry M.

doi:10.1614/wt-d-10-00150.1

Cited by 68 publications

(69 citation statements)

References 15 publications

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“…In atrazine-resistant A. retroflexus (possessing an insensitive D1 protein) (Hugie et al, 2008), PRE atrazine followed by POST mesotrione treatments demonstrated a synergistic response, whereas in atrazine-resistant A. theophrasti possessing enhanced GST-mediated metabolism of atrazine, only the POST tank-mix displayed a synergistic response (Woodyard et al, 2009b). However, recent discoveries of mesotrione-and atrazine-resistant waterhemp populations, such as the MCR population from Illinois (Hausman et al, 2011(Hausman et al, , 2016 and a waterhemp population from Iowa (McMullan and Green, 2011), may diminish the ability to achieve POST synergism with HPPD inhibitor-atrazine combinations and, consequently, limit effective POST control options for multiple herbicide-resistant (MHR) waterhemp in maize.…”

Section: Core Ideasmentioning

confidence: 99%

Differential Responses to Preemergence and Postemergence Atrazine in Two Atrazine‐Resistant Waterhemp Populations

Evans

Riechers

2016

Agronomy Journal

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Waterhemp [Amaranthus tuberculatus (Moq) Sauer] is a difficult‐to‐control dicot weed in the United States. Atrazine [6‐chloro‐N‐ethyl‐N’‐(1‐methylethyl)‐1,3,5‐triazine‐2,4‐diamine] is commonly used for preemergence (PRE) and postemergence (POST) waterhemp control in maize (Zea mays L.). Previous research reported that atrazine metabolism via glutathione S‐transferase (GST) activity contributes to atrazine POST resistance in two waterhemp populations from Illinois, designated MCR (McLean County, Illinois, resistant) and ACR (Adams County, Illinois, resistant). Objectives were to quantify responses of these populations to atrazine PRE and determine if the combination of a GST inhibitor and atrazine PRE or POST increases their control. Dose‐response analyses indicated MCR was resistant to atrazine PRE relative to ACR or WCS (Wayne County sensitive; herbicide‐sensitive population), despite MCR and ACR exhibiting equivalent levels of atrazine resistance POST. The ACR response to atrazine PRE (LD50) was intermediate compared with MCR and WCS. Seedling survival of ACR was reduced by 4‐chloro‐7‐nitrobenzofurazan (NDB‐Cl; a GST inhibitor) and atrazine PRE more than atrazine PRE alone, but not in MCR. Atrazine following NBD‐Cl applied POST inhibited seedling growth in ACR, but not in MCR. Enhanced atrazine activity with NBD‐Cl further supports rapid metabolism via GSTs as the main atrazine‐resistance mechanism in ACR. GST(s) that metabolize atrazine in MCR may not have been completely inhibited by NBD‐Cl, indicating that similar yet distinct atrazine‐resistance mechanisms exist in MCR compared to ACR. In conclusion, atrazine PRE (with or without NBD‐Cl) still controls ACR when applied at typical field‐use rates in maize, but the length of residual activity may be shorter than in sensitive populations.Core Ideas Two waterhemp populations are resistant to atrazine POST due to increased metabolism by GST enzymes. Since atrazine can also be soil applied, quantifying the levels of resistance in these populations is important for weed management in maize. The MCR population was resistant to atrazine PRE and displayed a higher level of resistance to atrazine PRE than the ACR population. NBD‐Cl, a metabolic inhibitor of GST enzymes, enhanced atrazine activity in the ACR population but not MCR. The length of residual activity of atrazine may be shorter for controlling these populations in maize compared with sensitive populations.

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Section: Core Ideasmentioning

confidence: 99%

Differential Responses to Preemergence and Postemergence Atrazine in Two Atrazine‐Resistant Waterhemp Populations

Evans

Riechers

2016

Agronomy Journal

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“…MCR displayed 10-and 35-fold resistance to mesotrione in comparison with ACR and WCS, respectively, in greenhouse studies (Hausman et al, 2011). In addition, waterhemp populations with similar patterns of multiple resistance have recently been identified (Hausman et al, 2011;McMullan and Green, 2011;Heap, 2012). However, the mechanisms of resistance to mesotrione and atrazine in these waterhemp populations are currently unknown.…”

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Distinct Detoxification Mechanisms Confer Resistance to Mesotrione and Atrazine in a Population of Waterhemp

et al. 2013

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Previous research reported the first case of resistance to mesotrione and other 4-hydroxyphenylpyruvate dioxygenase (HPPD) herbicides in a waterhemp (Amaranthus tuberculatus) population designated MCR (for McLean County mesotrione-and atrazineresistant). Herein, experiments were conducted to determine if target site or nontarget site mechanisms confer mesotrione resistance in MCR. Additionally, the basis for atrazine resistance was investigated in MCR and an atrazine-resistant but mesotrione-sensitive population (ACR for Adams County mesotrione-sensitive but atrazine-resistant). A standard sensitive population (WCS for Wayne County herbicide-sensitive) was also used for comparison. Mesotrione resistance was not due to an alteration in HPPD sequence, HPPD expression, or reduced herbicide absorption. Metabolism studies using whole plants and excised leaves revealed that the time for 50% of absorbed mesotrione to degrade in MCR was significantly shorter than in ACR and WCS, which correlated with previous phenotypic responses to mesotrione and the quantity of the metabolite 4-hydroxy-mesotrione in excised leaves. The cytochrome P450 monooxygenase inhibitors malathion and tetcyclacis significantly reduced mesotrione metabolism in MCR and corn (Zea mays) excised leaves but not in ACR. Furthermore, malathion increased mesotrione activity in MCR seedlings in greenhouse studies. These results indicate that enhanced oxidative metabolism contributes significantly to mesotrione resistance in MCR. Sequence analysis of atrazine-resistant (MCR and ACR) and atrazine-sensitive (WCS) waterhemp populations detected no differences in the psbA gene. The times for 50% of absorbed atrazine to degrade in corn, MCR, and ACR leaves were shorter than in WCS, and a polar metabolite of atrazine was detected in corn, MCR, and ACR that cochromatographed with a synthetic atrazineglutathione conjugate. Thus, elevated rates of metabolism via distinct detoxification mechanisms contribute to mesotrione and atrazine resistance within the MCR population.

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“…In addition, just in the last six years it was confirmed that waterhemp biotypes evolved resistance to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides (WSSA SOA Group 27) in Illinois (Hausman et al 2011) and Iowa (McMullan and Green 2011). Therefore, new herbicide options to manage waterhemp are needed .…”

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confidence: 99%

Confirmation and Control of HPPD-Inhibiting Herbicide–Resistant Waterhemp (Amaranthus tuberculatus) in Nebraska

et al. 2017

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Field and greenhouse experiments were conducted in Nebraska to (1) confirm the 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting resistant-waterhemp biotype (HPPD-RW) by quantifying the resistance levels in dose-response studies, and (2) to evaluate efficacy of PRE-only, POST-only, and PRE followed by POST herbicide programs for control of HPPD-RW in corn. Greenhouse dose-response studies confirmed that the suspected waterhemp biotype in Nebraska has evolved resistance to HPPD-inhibiting herbicides with a 2-to 18-fold resistance depending upon the type of HPPD-inhibiting herbicide being sprayed. Under field conditions, at 56 d after treatment, ≥90% control of the HPPD-RW was achieved with PRE-applied mesotrione/atrazine/ S-metolachlor + acetochlor, pyroxasulfone (180 and 270 g ai ha −1 ), pyroxasulfone/fluthiacet-methyl/ atrazine, and pyroxasulfone + saflufenacil + atrazine. Among POST-only herbicide programs, glyphosate, a premix of mesotrione/atrazine tank-mixed with diflufenzopyr/dicamba, or metribuzin, or glufosinate provided ≥92% HPPD-RW control. Herbicide combinations of different effective sites of action in mixtures provided ≥86% HPPD-RW control in PRE followed by POST herbicide programs. It is concluded that the suspected waterhemp biotype is resistant to HPPD-inhibiting herbicides and alternative herbicide programs are available for effective control in corn. The occurrence of HPPD-RW in Nebraska is significant because it limits the effectiveness of HPPD-inhibiting herbicides. Nomenclature: Acetochlor, atrazine, glyphosate, clopyralid, dicamba, diflufenzopyr, dimethenamid-P, flumetsulam, fluthiacet-methyl, glufosinate, isoxaflutole, mesotrione, metribuzin, pyroxasulfone S-metolachlor, saflufenacil, rimsulfuron, tembotrione, thiencarbazone-methyl, topramezone, waterhemp, Amaranthus tuberculatus (Moq.) Sauer, corn, Zea mays L. Key words: 4-hydroxyphenylpyruvate dioxygenase, pigment inhibitors, PRE, POST, triketone, weed management, weed resistance. Se realizaron experimentos de campo y de invernadero en Nebraska para (1) confirmar un biotipo de Amaranthus tuberculatus resistente a inhibidores de 4-hydroxyphenylpyruvate dioxygenase (HPPD) (HPPD-RW) cuantificando el nivel de resistencia con estudios de respuesta a dosis, y (2) evaluar la eficacia de programas de herbicidas para el control de HPPD-RW en maíz con sólo herbicidas PRE, sólo POST, y herbicidas PRE seguidos por POST. Los estudios de respuesta a dosis en invernadero confirmaron que el biotipo de A. tuberculatus en Nebraska ha evolucionado resistencia a herbicidas inhibidores de HPPD con 2 a 18 veces mayor resistencia dependiendo del tipo de herbicida inhibidor de HPPD que se aplicó. Bajo condiciones de campo, a 56 d después del tratamiento, se alcanzó ≥90% de control de HPPD RW con aplicaciones PRE de mesotrione/ atrazine/S-metolachlor + acetochlor, pyroxasulfone (180 y 270 g ai ha), pyroxasulfone/fluthiacet-methyl/atrazine, y pyroxasulfone + saflufenacil + atrazine. Entre los programas de herbicidas con sólo POST, glyphosate, una premezcla d...

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Identification of a Tall Waterhemp (Amaranthus tuberculatus) Biotype Resistant to HPPD-Inhibiting Herbicides, Atrazine, and Thifensulfuron in Iowa

Cited by 68 publications

References 15 publications

Differential Responses to Preemergence and Postemergence Atrazine in Two Atrazine‐Resistant Waterhemp Populations

Differential Responses to Preemergence and Postemergence Atrazine in Two Atrazine‐Resistant Waterhemp Populations

Distinct Detoxification Mechanisms Confer Resistance to Mesotrione and Atrazine in a Population of Waterhemp

Confirmation and Control of HPPD-Inhibiting Herbicide–Resistant Waterhemp (Amaranthus tuberculatus) in Nebraska

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