Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta

Fan, Lu; Kishore, Anusha; Jansen-Olliges, Linda; Wang, Dahua; Stahl, Frank; Psathaki, Olympia E.; Harre, Jennifer; Warnecke, Athanasia; Weder, Julia; Preller, Matthias; Zeilinger, Carsten

doi:10.1021/acsomega.2c02331

Cited by 3 publications

(2 citation statements)

References 82 publications

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“…We will also aim to lower the limit of detection. Furthermore, the QD concentration in the spots can be optimised, by adding an additional layer of antibodies or proteins such as Thyroid hormone receptor beta binding to Hsp90 [45]. Also, the background noise and coffee ring effect might be reduced by using different substrates for the biochip.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

Kishore,

Varughese,

Roth

et al. 2024

Preprint

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We report on a simplified optical imager to detect the presence of a stress biomarker protein, namely the Heat shock protein 90 (Hsp90). The imager consists of two elements the optical unit and the sensor which is a custom-made biochip. Measurement is based on the masking of the streptavidin conjugated quantum dot’s (Sav-QDs) fluorescence when Hsp90 attaches to it via biotinylated antibodies (Ab). The masking effect was directly proportional to the Hsp90 concentration. The cost-efficient benchtop imager developed comprises a CMOS sensor, standard optical lenses, and a narrow bandpass filter for optically eliminating background fluorescence. This approach is promising for the realization of cheap, robust, and reliable point-of-care detection systems for various biomarker analysis.<p></p>

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Section: Discussionmentioning

confidence: 99%

Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

Kishore,

Varughese,

Roth

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…We will also aim to lower the limit of detection. Furthermore, the QD concentration in the spots can be optimized, by adding an additional layer of antibodies or proteins such as Thyroid hormone receptor beta binding to Hsp90 [ 44 ]. Also, the background noise and coffee ring effect might be reduced by using different substrates for the biochip.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

Kishore,

Varughese,

Roth

et al. 2024

Biomed. Opt. Express

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We report on a simplified optical imager to detect the presence of a stress biomarker protein, namely the Heat shock protein 90 (Hsp90). The imager consists of two elements the optical unit and the sensor, which is a custom-made biochip. Measurement is based on the masking of the streptavidin conjugated quantum dot’s (Sav-QDs) fluorescence when Hsp90 attaches to it via biotinylated antibodies (Ab). The masking effect was directly proportional to the Hsp90 concentration. The cost-efficient benchtop imager developed comprises a CMOS sensor, standard optical lenses, and a narrow bandpass filter for optically eliminating background fluorescence. This approach is promising for the realization of cheap, robust, and reliable point-of-care detection systems for various biomarker analyses.

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Fluorescence Masking Based Multifunctional Quantum Dots’ Assay for HSP90α Interactions Detection

et al. 2023

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HSP90α is one of the most common stress proteins in cells; hence, it is a good target for developing drugs and testing systems for cancer or physical stress levels in humans. Streptavidin conjugated quantum dots (Sav-QDs) are widely used as fluorophores for biosensing to overcome chemical labelling problems. In this work, we have attempted to develop a multifunctional and robust assay for HSP90α. The detection technique was based on the masking of the fluorescence of spotted Sav-QDs on nitrocellulose chips (NC). Biotinylated ligand/antibody attaches to the spotted Sav-QD and then HSP90α is attached, which causes the masking of fluorescence. The masking of fluorescence was used to detect protein–ligand interactions, the effect of inhibitors, protein–protein interactions, and the presence of protein in the biological sample. The load of detection (LoD) of the assay lies in the nano molar range, making it a sensitive assay. The results from the experiments suggest that the used approach is promising for developing a multifunctional, robust, and sensitive assay for proteins that can be used for point-of-care detection in complex biological samples.

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Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta

Cited by 3 publications

References 82 publications

Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

Fluorescence Masking Based Multifunctional Quantum Dots’ Assay for HSP90α Interactions Detection

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