Identification of a Transcription Factor, BHLHB8, Involved in Mouse Seminal Vesicle Epithelium Differentiation and Function1

Pin, Christopher L.; Johnson, C. L.; Rade, Bryan; Kowalik, Agnes S.; Garside, Victoria C.; Everest, Michelle

doi:10.1095/biolreprod.107.064196

Cited by 5 publications

(5 citation statements)

References 58 publications

(89 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results from this study suggest that mutations in MIST1 could also be linked to the disease. A complete absence of MIST1 is not deleterious to overall health, reproductive ability, viability, and survival of the mice [17], [33] indicating that altered MIST1 function can go unnoticed for long periods of time. While a small percentage of Mist1 −/− mice do develop more overt fibrosis and inflammation characteristic of chronic pancreatitis, the majority (95%) of the mice are relatively unaffected.…”

Section: Discussionmentioning

confidence: 98%

The Absence of MIST1 Leads to Increased Ethanol Sensitivity and Decreased Activity of the Unfolded Protein Response in Mouse Pancreatic Acinar Cells

et al. 2011

Self Cite

View full text Add to dashboard Cite

BackgroundAlcohol abuse is a leading cause of pancreatitis in humans. However, rodent models suggest that alcohol only sensitizes the pancreas to subsequent insult, indicating that additional factors play a role in alcohol-induced pancreatic injury. The goal of this study was to determine if an absence of MIST1, a transcription factor required for complete differentiation of pancreatic acinar cells in mice, increased the sensitivity to alcohol.MethodsTwo to four month-old mice lacking MIST1 (Mist1−/−) or congenic C57 Bl6 mice were placed on a Lieber-DeCarli diet (36% of total kcal from ethanol and fat), a control liquid diet (36% kcal from fat) or a regular breeding chow diet (22% kcal from fat). After six weeks, pancreatic morphology was assessed. Biochemical and immunofluorescent analysis was used to assess mediators of the unfolded protein response (UPR).ResultsEthanol-fed Mist1−/− mice developed periductal accumulations of inflammatory cells that did not appear in wild type or control-fed Mist1−/− mice. Wild type mice fed diets high in ethanol or fat showed enhancement of the UPR based on increased accumulation of peIF2α and spliced XBP1. These increases were not observed in Mist1−/− pancreatic tissue, which had elevated levels of UPR activity prior to diet exposure. Indeed, exposure to ethanol resulted in a reduction of UPR activity in Mist1−/− mice.ConclusionsOur findings suggest that an absence of MIST1 increases the sensitivity to ethanol that correlated with decreased activity of the UPR. Therefore, events that affect the expression and/or function of MIST1 may be confounding factors in pancreatitis.

show abstract

Section: Discussionmentioning

confidence: 98%

The Absence of MIST1 Leads to Increased Ethanol Sensitivity and Decreased Activity of the Unfolded Protein Response in Mouse Pancreatic Acinar Cells

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…At grade 6, at which point sperm analysis using ejaculated semen collected by electroejaculation stimulation was possible, development of the epithelial cell layer including glandular secretions was noted in the seminal vesicle. These secretions produce most of the seminal fluid that affects sperm capacitation and motility, and toxicity on the fluid or fluid production may have an effect on male fertility (Pin et al 2008; Coroner et al 1992; de Lamirande et al 2001; Huang, Chu, and Chen 2000; Kawano and Yoshida 2007). Therefore, considering the notable development of the epithelial layer in the prostate and seminal vesicle from grade 5 and through grade 6, as well as that of the accessory reproductive organs, thorough evaluation of male reproductive toxicity was considered feasible at grade 6.…”

Section: Discussionmentioning

confidence: 99%

“…In mice, most of the branching in the seminal vesicle is complete at approximately 2 weeks after birth, and at this stage, epithelial cell differentiation begins and cells assume a highly secretory function. Through hypertrophy, the gland continues to grow into adulthood (Pin et al 2008). Brewster (1985) reported that in humans, at 18 years of age, seminal vesicles showed extensive epithelial folding around the circumference of the lumen, and at 45 years of age, there was a great increase in the extent of the epithelial folding in the seminal vesicle when compared with that in the specimen from the 18-year-old.…”

Section: Discussionmentioning

confidence: 99%

Morphometric Examination for Development of Reproductive Organs in Male Cynomolgus Monkeys

et al. 2012

View full text Add to dashboard Cite

We previously reported on a histological classification of cynomolgus monkey testis into six grades (1, immature; 2, prepuberty; 3, onset of puberty; 4, puberty; 5, early adult; 6, adult) based on spermatogenesis development. In this investigation, the accessory reproductive organs from the same animals underwent histomorphometric examination, in addition to being examined histologically and weighed, to evaluate relationships between these parameters and the six grades. Seminiferous tubule diameter increased corresponding to the testicular maturity grade and was notably increased at grade 6. Beginning from grade 3, increases in the areas of the ductus epididymis were noted, and reserved sperm was visible in the lumen. In the prostate, the glandular lumen area per unit area showed an increase beginning from grade 3 but no clear differences between grades 4 and 6; advanced development of epithelial height was observed at grade 6. In the seminal vesicle, development of the epithelial cell layer was markedly increased at grade 6. It was concluded that development of the male accessory reproductive organs began after reserved sperm was observed in the lumen of the ductus epididymis (grade 3) and that these organs were developed notably when the testis reached sexual maturity (grade 6).

show abstract

“…The Drosophila homolog for MIST1 is dimmed (6) and the gene nomenclature in mice has recently changed from Bhlhb8 (17) to Bhlha15. MIST1 is expressed in serous exocrine cells including acinar cells in the pancreas (16).…”

Section: General Functionmentioning

confidence: 99%

“…An absence of MIST1 also affects the differentiation and/or morphology of other serous exocrine cells include salivary glands (9), gastric epithelium (9,20), mammary gland alveolar cells (25), seminal vesicle (17), and plasma cells (2, 3).…”

Section: Mist1 In Pancreasmentioning

confidence: 99%

Mist1

The Pancreapedia: Exocrine Pancreas Knowledge Base

View full text Add to dashboard Cite

Identification of a Transcription Factor, BHLHB8, Involved in Mouse Seminal Vesicle Epithelium Differentiation and Function1

Cited by 5 publications

References 58 publications

The Absence of MIST1 Leads to Increased Ethanol Sensitivity and Decreased Activity of the Unfolded Protein Response in Mouse Pancreatic Acinar Cells

The Absence of MIST1 Leads to Increased Ethanol Sensitivity and Decreased Activity of the Unfolded Protein Response in Mouse Pancreatic Acinar Cells

Morphometric Examination for Development of Reproductive Organs in Male Cynomolgus Monkeys

Mist1

Contact Info

Product

Resources

About