Identification of a transforming growth factor-β type I receptor transcript in Eriocheir sinensis and its molting-related expression in muscle tissues

Tian, Zhong; Peng, Hanyong; Deng, Weide; Jiao, Chuanzhen

doi:10.1007/s11033-019-05108-8

Cited by 6 publications

(6 citation statements)

References 47 publications

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“…In addition, a transforming growth factor-b type I receptor (EsTGFBRI) might play roles in molting-related muscle growth in E. sinensis. EsTGFBRI in claw and abdominal muscles in the later pre-molt stage were significantly higher than those in the inter-and post-molt stages (Tian et al, 2020). However, the molecular mechanism of muscle discontinuous growth during molting is still unclear.…”

Section: Introductionmentioning

confidence: 92%

Integration of ATAC-seq and RNA-seq identifies active G-protein coupled receptors functioning in molting process in muscle of Eriocheir sinensis

Sun

Li²,

Lv³

et al. 2022

Front. Mar. Sci.

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Discontinuous muscle growth during molting is an important feature of Eriocheir sinensis. Molting is a physiological process completed by the cooperation of multiple organs. Signal transmission is critical for the accurate regulation of each step in molting. However, the knowledge of the signal transduction mechanism in the molting process of E. sinensis is presently very limited. In this work, the chromatin accessibility and gene expression of the muscle in E. sinensis in pre-molt (D) and post-molt (A) stages were sequenced by assay of transposase accessible chromatin sequencing (ATAC-seq) and RNA-seq, respectively. The differentially expressed genes (DEGs) in the muscle before and after molting were analyzed by combining ATAC-seq and RNA-seq, especially the G-protein coupled receptor (GPCR) genes in the process of signal transduction. The results showed that there were 616 common DEGs in ATAC-seq and RNA-seq in A vs. D stages, of which 538 were upregulated and 78 were downregulated. In the 19 DEGs included in the signaling transduction process, 13 were located in the GPCR signaling pathway and all were upregulated in A stages, which indicated that GPCRs play a leading role in muscle signal transmission during post-molt stage in molting. In these genes, the structure of the proteins encoded by 10 membrane-located genes with transmembrane activity was further analyzed. Six candidate GPCR genes were finally identified and further verified by real-time quantitative PCR (qRT-PCR). The GPCRs include metabotropic glutamate receptor 7, Mth-like 4, and Mth2 proteins. These results show the existence of GPCRs in the muscle of E. sinensis and, for the first time, found their dominant role in the signal transduction process during molting. It provides important clues for the study of muscle discontinuous growth and molting mechanism of E. sinensis.

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Section: Introductionmentioning

confidence: 92%

Integration of ATAC-seq and RNA-seq identifies active G-protein coupled receptors functioning in molting process in muscle of Eriocheir sinensis

Sun

Li²,

Lv³

et al. 2022

Front. Mar. Sci.

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“…S6 kinase also plays an important regulatory role in muscle growth during E. sinensis molting process (Tian et al, 2019;Hou et al, 2020;Tian et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…( Yu et al, 2018 ; Chen et al, 2019 ; Wang et al, 2020a ; Liu et al, 2021a ; Zhang et al, 2021 ). Moreover, the role of several regulatory genes such as V-ATPase subunit B (VATB), transforming growth factor-beta type I receptor (TGFBR1) and S6 kinase has also been studied ( Tian et al, 2019 ; Hou et al, 2020 ; Tian et al, 2020 ). The results indicated that V-ATPase subunit B plays essential roles in the cuticle formation process of Eriocheir sinensis .…”

Section: Introductionmentioning

confidence: 99%

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Exploration of the regulatory mechanisms of regeneration, anti-oxidation, anti-aging and the immune response at the post-molt stage of Eriocheir sinensis

Wang

et al. 2022

Front. Physiol.

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Eriocheir sinensis is widely appreciated by the surrounding population due to its culinary delicacy and rich nutrients. The E. sinensis breeding industry is very prosperous and molting is one of the important growth characteristics. Research on the regulation of molting in E. sinensis is still in the initial stages. There is currently no relevant information on the regulatory mechanisms of heart development following molting. Comparative transcriptome analysis was used to study developmental regulation mechanisms in the heart of E. sinensis at the post-molt and inter-molt stages. The results indicated that many regulatory pathways and genes involved in regeneration, anti-oxidation, anti-aging and the immune response were significantly upregulated after molting in E. sinensis. Aside from cardiac development, the differentially expressed genes (DEGs) were relevant to myocardial movement and neuronal signal transduction. DEGs were also related to the regulation of glutathione homeostasis and biological rhythms in regard to anti-oxidation and anti-aging, and to the regulation of immune cell development and the immune response. This study provides a theoretical framework for understanding the regulation of molting in E. sinensis and in other economically important crustaceans.

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“…The Chinese mitten crab ( Eriocheir sinensis ) is an important economic crab species with huge aquaculture industry in China; however, it is considered an invasive species in Europe and North America ( Huang et al, 2015 ; Wang et al, 2018 ). Recently, the mRNA structure and expression of TGFBR1 were reported in E. sinensis , and the functions of TGF-β-like signaling mediated by TGFBR1 in molting-related muscle growth were elucidated in crustaceans ( Tian et al, 2019b ). As an important receptor of TGF-β superfamily members, the structure, expression, and biological actions of ActRIIB in regulating growth and muscle mass have not been identified in E. sinensis .…”

Section: Introductionmentioning

confidence: 99%

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

Wang

Zhang

Hou

et al. 2020

PeerJ

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Activin receptor IIB (ActRIIB) is a serine/threonine-kinase receptor binding with transforming growth factor-β (TGF-β) superfamily ligands to participate in the regulation of muscle mass in vertebrates. However, its structure and function in crustaceans remain unknown. In this study, the ActRIIB gene in Eriocheir sinensis (Es-ActRIIB) was cloned and obtained with a 1,683 bp open reading frame, which contains the characteristic domains of TGF-β type II receptor superfamily, encoding 560 amino acids. The mRNA expression of Es-ActRIIB was the highest in hepatopancreas and the lowest in muscle at each molting stage. After injection of Es-ActRIIB double-stranded RNA during one molting cycle, the RNA interference (RNAi) group showed higher weight gain rate, higher specific growth rate, and lower hepatopancreas index compared with the control group. Meanwhile, the RNAi group displayed a significantly increased content of hydrolytic amino acid in both hepatopancreas and muscle. The RNAi group also displayed slightly higher contents of saturated fatty acid and monounsaturated fatty acid but significantly decreased levels of polyunsaturated fatty acid compared with the control group. After RNAi on Es-ActRIIB, the mRNA expressions of five ActRIIB signaling pathway genes showed that ActRI and forkhead box O (FoxO) were downregulated in hepatopancreas and muscle, but no significant expression differences were found in small mother against decapentaplegic (SMAD) 3, SMAD4 and mammalian target of rapamycin. The mRNA expression s of three lipid metabolism-related genes (carnitine palmitoyltransferase 1β (CPT1β), fatty acid synthase, and fatty acid elongation) were significantly downregulated in both hepatopancreas and muscle with the exception of CPT1β in muscles. These results indicate that ActRIIB is a functionally conservative negative regulator in growth mass, and protein and lipid metabolism could be affected by inhibiting ActRIIB signaling in crustacean.

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Identification of a transforming growth factor-β type I receptor transcript in Eriocheir sinensis and its molting-related expression in muscle tissues

Cited by 6 publications

References 47 publications

Integration of ATAC-seq and RNA-seq identifies active G-protein coupled receptors functioning in molting process in muscle of Eriocheir sinensis

Integration of ATAC-seq and RNA-seq identifies active G-protein coupled receptors functioning in molting process in muscle of Eriocheir sinensis

Exploration of the regulatory mechanisms of regeneration, anti-oxidation, anti-aging and the immune response at the post-molt stage of Eriocheir sinensis

Molecular characteristic of activin receptor IIB and its functions in growth and nutrient regulation in Eriocheir sinensis

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