Identification of a tumor suppressive critical region mapping to 3p14.2 in esophageal squamous cell carcinoma and studies of a candidate tumor suppressor gene, ADAMTS9

Lo, Paulisally Hau Yi; Leung, Alfred Chi Chung; Kwok, C Y C; Cheung, Wai-Man; Ko, Josephine Mun Yee; Li, Yang; Law, Simon; Wang, L D; Li, J; Stanbridge, Eric J.; Srivastava, Gopesh; Tang, Johnny; Tsao, Sai Wah; Lung, Maria Li

doi:10.1038/sj.onc.1209767

Cited by 71 publications

(76 citation statements)

References 33 publications

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“…As mentioned in the previous section, ADAMTS9 was identified as one of the differentially expressed genes in these non-tumorigenic MCHs and their matched TS cell lines in our previous study (Lo et al 2007). It was suggested that ADAMTS9 is associated with tumor suppression in human esophageal cancer.…”

Section: Adamts9 (A Disintegrin-like and Metalloprotease With Thrombomentioning

confidence: 99%

“…However, we only introduce a few of examples of these studies in this chapter due to the space limitation. (Cheng et al, 2000, 2002, 2003, Lung et al 2008aand 2008b, Cheung et al 2009, Lo et al 2007and 2010 4.1 Chromosome 3: ADAMTS9 In a subsequent study, an intact and two truncated human chromosomes 3 obtained from the same panel of chromosome 3 donor cells, were transferred into the highly tumorigenic ESCC SLMT-1 cell line. Similarly, the ability of these transferred chromosomes to functionally complement defects in the ESCC cell line was assessed by examining the impact of this transfer on tumorigenic potential in nude mice.…”

Section: Examples Of Candidate Tsgs Studied Via Mmct and Other Functimentioning

confidence: 99%

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Identification of Tumor Suppressor Genes via Cell Fusion and Chromosomal Transfer

Lung¹,

Cheung²,

Ko³

et al. 2012

Tumor Suppressor Genes

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Section: Adamts9 (A Disintegrin-like and Metalloprotease With Thrombomentioning

confidence: 99%

Section: Examples Of Candidate Tsgs Studied Via Mmct and Other Functimentioning

confidence: 99%

Identification of Tumor Suppressor Genes via Cell Fusion and Chromosomal Transfer

Lung¹,

Cheung²,

Ko³

et al. 2012

Tumor Suppressor Genes

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“…EC18 and KYSE180 were treated with 5 Amol/L 5-aza-2 ¶-deoxycytidine (Sigma), as previously described (10). On the 5th day of 5-aza-2 ¶-deoxycytidine treatment, 300 nmol/L trichostatin A (Sigma) was added for 24 h before cell harvesting.…”

Section: -Aza-2 ¶-Deoxycytidine and Trichostatin A Treatmentmentioning

confidence: 99%

“…Culture conditions for the SLMT-1, donor, MCH, and TS cell lines, and details for a panel of 16 ESCC cell lines were described previously (10,12). NE1, a human papillomavirus E6/E7/telomerase -immortalized normal esophageal epithelial cell line (23), is the reference cell line for gene expression profile analysis in the microarray and RT-PCR studies.…”

Section: Cell Lines and Culture Conditionsmentioning

confidence: 99%

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Monochromosome Transfer and Microarray Analysis Identify a Critical Tumor-Suppressive Region Mapping to Chromosome 13q14 and THSD1 in Esophageal Carcinoma

Chan

Yau

et al. 2008

Molecular Cancer Research

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Loss of chromosome 13q regions in esophageal squamous cell carcinoma (ESCC) is a frequent event. Monochromosome transfer approaches provide direct functional evidence for tumor suppression by chromosome 13 in SLMT-1, an ESCC cell line, and identify critical regions at 13q12.3, 13q14.11, and 13q14.3. Differential gene expression profiles of three tumor-suppressing microcell hybrids (MCH) and their tumorigenic parental SLMT-1 cell line were revealed by competitive hybridization using 19k cDNA oligonucleotide microarrays. Nine candidate 13q14 tumor-suppressor genes (TSG), including RB1, showed down-regulation in SLMT-1, compared with NE1, an immortalized normal esophageal epithelial cell line; their average gene expression was restored in MCHs compared with SLMT-1. Reverse transcription-PCR validated gene expression levels in MCHs and a panel of ESCC cell lines. Results suggest that the tumor-suppressing effect is not attributed to RB1, but instead likely involves thrombospondin type I domain-containing 1 (THSD1), a novel candidate TSG mapping to 13q14. Quantitative reverse transcription-PCR detected down-regulation of THSD1 expression in 100% of ESCC and other cancer cell lines. Mechanisms for THSD1 silencing in ESCC involved loss of heterozygosity and promoter hypermethylation, as analyzed by methylation-specific PCR and clonal bisulfite sequencing. Transfection of wild-type THSD1 into SLMT-1 resulted in significant reduction of colony-forming ability, hence providing functional evidence for its growth-suppressive activity. These findings suggest that THSD1 is a good candidate TSG.

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Increased mRNA expression of ADAMTS metalloproteinases in metastatic foci of head and neck cancer

Demircan

Gündüz

et al. 2009

Head & Neck

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Identification of a tumor suppressive critical region mapping to 3p14.2 in esophageal squamous cell carcinoma and studies of a candidate tumor suppressor gene, ADAMTS9

Cited by 71 publications

References 33 publications

Identification of Tumor Suppressor Genes via Cell Fusion and Chromosomal Transfer

Identification of Tumor Suppressor Genes via Cell Fusion and Chromosomal Transfer

Monochromosome Transfer and Microarray Analysis Identify a Critical Tumor-Suppressive Region Mapping to Chromosome 13q14 and THSD1 in Esophageal Carcinoma

Increased mRNA expression of ADAMTS metalloproteinases in metastatic foci of head and neck cancer

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