Identification of A-type allatostatins possessing −YXFGI/Vamide carboxy-termini from the nervous system of the copepod crustacean Calanus finmarchicus

Christie, Andrew E.; Sousa, Gregory L.; Rus, Szymon; Smith, Christine M.; Towle, David W.; Hartline, Daniel K.; Dickinson, Patsy S.

doi:10.1016/j.ygcen.2007.09.002

Cited by 34 publications

(28 citation statements)

References 32 publications

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“…With respects to nervous system development in copepodids, some relevant genes such as nicotinic acetylcholine receptor, flotillin, synaptotagmin, allotostatin, frequenin, and opsin were highly overexpressed. These results are congruent with previous studies of transcriptome profiles reported for copepodid stages [29], [45], [46]. Furthermore, a study by Wilson and Hartline [47] demonstrated high peripheral and central nervous system development in individuals transitioning from the nauplius to copepodid stage.…”

Section: Discussionsupporting

confidence: 92%

RNA-Seq Analysis Using De Novo Transcriptome Assembly as a Reference for the Salmon Louse Caligus rogercresseyi

2014

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Despite the economic and environmental impacts that sea lice infestations have on salmon farming worldwide, genomic data generated by high-throughput transcriptome sequencing for different developmental stages, sexes, and strains of sea lice is still limited or unknown. In this study, RNA-seq analysis was performed using de novo transcriptome assembly as a reference for evidenced transcriptional changes from six developmental stages of the salmon louse Caligus rogercresseyi. EST-datasets were generated from the nauplius I, nauplius II, copepodid and chalimus stages and from female and male adults using MiSeq Illumina sequencing. A total of 151,788,682 transcripts were yielded, which were assembled into 83,444 high quality contigs and subsequently annotated into roughly 24,000 genes based on known proteins. To identify differential transcription patterns among salmon louse stages, cluster analyses were performed using normalized gene expression values. Herein, four clusters were differentially expressed between nauplius I–II and copepodid stages (604 transcripts), five clusters between copepodid and chalimus stages (2,426 transcripts), and six clusters between female and male adults (2,478 transcripts). Gene ontology analysis revealed that the nauplius I–II, copepodid and chalimus stages are mainly annotated to aminoacid transfer/repair/breakdown, metabolism, molting cycle, and nervous system development. Additionally, genes showing differential transcription in female and male adults were highly related to cytoskeletal and contractile elements, reproduction, cell development, morphogenesis, and transcription-translation processes. The data presented in this study provides the most comprehensive transcriptome resource available for C. rogercresseyi, which should be used for future genomic studies linked to host-parasite interactions.

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Section: Discussionsupporting

confidence: 92%

RNA-Seq Analysis Using De Novo Transcriptome Assembly as a Reference for the Salmon Louse Caligus rogercresseyi

2014

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“…16 sequences containing the signature of AST-A were present in the precursor, each with a size between 6–11 residues. This represents a greater number of copies than has been found previously in the insects (at 13–14 forms) [38], but fewer than in the majority of the crustaceans, where between 30 to more than 40 sequences have been characterised [39]–[41]. Each of the sequences appeared to be of a unique origin, which is in contrast to analyses in Macrobrachium rosenbergi [40] and Procambarus clarkii [39] where two AST-A sequences are present numerous times, indicating multiple duplication events.…”

Section: Resultsmentioning

confidence: 69%

Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias

et al. 2013

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BackgroundThe Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and, in particular, anticipate their responses to climate change effects in the warming seas around Antarctica.Methodology/Principal FindingsIllumina sequencing was used to produce a transcriptome of the ice krill. Analysis of the assembled contigs via two different methods, produced 36 new pre-pro-peptides, coding for 61 neuropeptides or peptide hormones belonging to the following families: Allatostatins (A, B et C), Bursicon (α and β), Crustacean Hyperglycemic Hormones (CHH and MIH/VIHs), Crustacean Cardioactive Peptide (CCAP), Corazonin, Diuretic Hormones (DH), the Eclosion Hormone (EH), Neuroparsin, Neuropeptide F (NPF), small Neuropeptide F (sNPF), Pigment Dispersing Hormone (PDH), Red Pigment Concentrating Hormone (RPCH) and finally Tachykinin. LC/MS/MS proteomics was also carried out on eyestalk extracts, which are the major site of neuropeptide synthesis in decapod crustaceans. Results confirmed the presence of six neuropeptides and six precursor-related peptides previously identified in the transcriptome analyses.ConclusionsThis study represents the first comprehensive analysis of neuropeptide hormones in a Eucarida non-decapod Malacostraca, several of which are described for the first time in a non-decapod crustacean. Additionally, there is a potential expansion of PDH and Neuropeptide F family members, which may reflect certain life history traits such as circadian rhythms associated with diurnal migrations and also the confirmation via mass spectrometry of several novel pre-pro-peptides, of unknown function. Knowledge of these essential hormones provides a vital framework for understanding the physiological response of this key Southern Ocean species to climate change and provides a valuable resource for studies into the molecular phylogeny of these organisms and the evolution of neuropeptide hormones.

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“…The identification of native A-ASTs from Carcinus maenas, e.g., AGPYSFGLamide, followed shortly thereafter [16], with additional isoforms subsequently identified from a number of other decapod species [17,25,46,48,[50][51][52][77][78][79]. A-ASTs have also been identified by transcriptomics from several lower crustaceans, specifically the copepod Calanus finmarchicus [80] and the cladoceran Daphnia pulex [22]; in Calanus, the predicted isoforms exhibit variant -YXFGI/Vamide C-termini, e.g., APYGFGIamide and pQ/QPYNFGVamide [80].…”

Section: A-type Allatostatinsmentioning

confidence: 99%

“…A-ASTs are broadly distributed within the nervous systems of crustaceans, including regions of synaptic neuropil [22,76,80,81] and neuroendocrine organs [1,22,80,81], suggesting that they function as both locally released autocrines/paracrines and circulating hormones.…”

Section: A-type Allatostatinsmentioning

confidence: 99%

Crustacean neuropeptides

Christie

Stemmler

Dickinson

2010

Cell. Mol. Life Sci.

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194

407

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Crustaceans have long been used for peptide research. For example, the process of neurosecretion was first formally demonstrated in the crustacean X-organ-sinus gland system, and the first fully characterized invertebrate neuropeptide was from a shrimp. Moreover, the crustacean stomatogastric and cardiac nervous systems have long served as models for understanding the general principles governing neural circuit functioning, including modulation by peptides. Here, we review the basic biology of crustacean neuropeptides, discuss methodologies currently driving their discovery, provide an overview of the known families, and summarize recent data on their control of physiology and behavior.

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Identification of A-type allatostatins possessing −YXFGI/Vamide carboxy-termini from the nervous system of the copepod crustacean Calanus finmarchicus

Cited by 34 publications

References 32 publications

RNA-Seq Analysis Using De Novo Transcriptome Assembly as a Reference for the Salmon Louse Caligus rogercresseyi

RNA-Seq Analysis Using De Novo Transcriptome Assembly as a Reference for the Salmon Louse Caligus rogercresseyi

Transcriptome and Peptidome Characterisation of the Main Neuropeptides and Peptidic Hormones of a Euphausiid: The Ice Krill, Euphausia crystallorophias

Crustacean neuropeptides

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