Identification of a σ
            <sup>B</sup>
            -Dependent Small Noncoding RNA in
            <i>Listeria monocytogenes</i>

Nielsen, Jørgen Feldbæk; Olsen, Anders Steno; Bonde, Mette; Valentin‐Hansen, Poul; Kallipolitis, Birgitte H.

doi:10.1128/jb.00740-08

Cited by 46 publications

(55 citation statements)

References 36 publications

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“…Specifically, we showed that the prfA* ⌬sigB strain had higher hemolytic activity (232 hemolytic units [HU]) than a prfA* strain (213.3 HU) ( Table 3). Low hemolytic activities were observed for the ⌬prfA and ⌬prfA ⌬sigB strains, consistent with the PrfA-dependent nature of hly transcription (6,54,57,71).…”

Section: Vol 77 2009mentioning

confidence: 55%

“…This hypothesis is supported by the fact that the L. monocytogenes B regulon includes Ͼ140 genes, including those encoding proteins with importance in stress response, virulence, transcriptional regulation, metabolism, and transport (59). B also transcribes genes that encode other regulators, including small RNAs (12,54) and proteins that affect RNA stability (e.g., Hfq) (13).…”

Section: Discussionmentioning

confidence: 75%

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Listeria monocytogenesσ^BModulates PrfA-Mediated Virulence Factor Expression

et al. 2009

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Section: Vol 77 2009mentioning

confidence: 55%

Section: Discussionmentioning

confidence: 75%

Listeria monocytogenesσ^BModulates PrfA-Mediated Virulence Factor Expression

et al. 2009

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“…They have been recognized to be important regulators in biological processes in eukaryotes (60) and bacteria (61,62). So far, a large repertoire of ncRNAs has been described in L. monocytogenes, and evidence suggests that they are central to environmental adaptation (31,(35)(36)(37)(38)(63)(64)(65). As in other bacterial models, this noncoding part of the genome is an important contributor to the regulation of virulence in L. monocytogenes (35)(36)(37)(38)(39).…”

Section: Figmentioning

confidence: 99%

Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation

Vivant

Garmyn

Gal

et al. 2015

Appl Environ Microbiol

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In this study, we investigated whether the Agr-mediated response is triggered during adaptation in soil, and we compared survival patterns in a set of 10 soils. The fate of the parental strain L. monocytogenes L9 (a rifampin-resistant mutant of L. monocytogenes EGD-e) and that of a ⌬agrA deletion mutant were compared in a collection of 10 soil microcosms. The ⌬agrA mutant displayed significantly reduced survival in these biotic soil microcosms, and differential transcriptome analyses showed large alterations of the transcriptome when AgrA was not functional, while the variations in the transcriptomes between the wild type and the ⌬agrA deletion mutant were modest under abiotic conditions. Indeed, in biotic soil environments, 578 protein-coding genes and an extensive repertoire of noncoding RNAs (ncRNAs) were differentially transcribed. The transcription of genes coding for proteins involved in cell envelope and cellular processes, including the phosphotransferase system and ABC transporters, and proteins involved in resistance to antimicrobial peptides was affected. Under sterilized soil conditions, the differences were limited to 86 genes and 29 ncRNAs. These results suggest that the response regulator AgrA of the Agr communication system plays important roles during the saprophytic life of L. monocytogenes in soil. Listeria monocytogenes is the causative agent of listeriosis, a serious foodborne infection affecting essentially immunocompromised individuals, the elderly, and pregnant women (1). The pathogen is largely spread in the environment. It has been isolated from water systems (2-4), vegetation (5), soil (6-8), farms (9-12), food industries (13-15), and the feces of animals (16-18). Environmental adaptation requires that the cell have the ability to integrate environmental cues in order to adapt its physiology to the surrounding conditions through the regulation of gene expression. Genomics showed that an important part of the L. monocytogenes genome (7.3%) is dedicated to regulation and includes 209 transcriptional regulators, 15 histidine kinases, and 16 response regulators constituting two-component systems (19). Two-component systems participate in the ability of bacteria to sense and respond to fluctuating environmental conditions. AgrC/AgrA is a two-component regulatory system that is part of the Agr communication system. Initially described in Staphylococcus aureus, this communication system is organized as a four-gene operon, agrBDCA. AgrB is a membrane-bound protein that processes the propeptide AgrD into a mature autoinducing peptide (AIP). Detection of AIP by the histidine kinase AgrC induces transcriptional regulation through activation of the regulator AgrA. Detailed data concerning the role of the Agr system in the physiology of S. aureus are available (20)(21)(22). So far, its role in the adaptation of L. monocytogenes to its environment is only partially understood (23). Reports show that the Agr communication system of L. monocytogenes is involved in adhesion to abiotic surfaces (24) in ...

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“…MicA and RybB regulating outer membrane proteins are dependent on the alternative sigma factor E (20,32,44,46) associated with membrane stress. Recently, a novel Listeria sRNA that is under the control of the stress sigma factor B was found (31). Here, we report the properties of a novel sRNA, SR2 (30), identified by our group using the same approach that was used previously for SR1 (24).…”

Section: Discussionmentioning

confidence: 89%

CodY Activates Transcription of a Small RNA inBacillus subtilis

et al. 2009

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Regulatory small RNAs (sRNAs) in bacterial genomes have become a focus of research over the past 8 years. Whereas more than 100 such sRNAs have been found in Escherichia coli, relatively little is known about sRNAs in gram-positive bacteria. Using a computational approach, we identified two sRNAs in intergenic regions of the Bacillus subtilis genome, SR1 and SR2 (renamed BsrF). Recently, we demonstrated that SR1 inhibits the translation initiation of the transcriptional activator AhrC. Here, we describe detection of BsrF, its expression profile, and its regulation by CodY. Furthermore, we mapped the secondary structure of BsrF. BsrF is expressed in complex and minimal media in all growth phases in B. subtilis and, with a similar expression profile, also in Bacillus amyloliquefaciens. Neither overexpression nor deletion of bsrF affected the growth of B. subtilis. BsrF was found to be long-lived in complex and minimal media. Analysis of 13 putative transcription factor binding sites upstream of bsrF revealed only an effect for CodY. Here, we showed by using Northern blotting, lacZ reporter gene fusions, in vitro transcription, and DNase I footprinting that the transcription of bsrF is activated by CodY in the presence of branched-chain amino acids and GTP. Furthermore, BsrF transcription was increased 1.5-to 2-fold by glucose in the presence of branched-chain amino acids, and this increase was independent of the known glucose-dependent regulators. BsrF is the second target for which transcriptional activation by CodY has been discovered.

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Identification of a σ ^B -Dependent Small Noncoding RNA in Listeria monocytogenes

Cited by 46 publications

References 36 publications

Listeria monocytogenesσ^BModulates PrfA-Mediated Virulence Factor Expression

Listeria monocytogenesσ^BModulates PrfA-Mediated Virulence Factor Expression

Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation

CodY Activates Transcription of a Small RNA inBacillus subtilis

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Identification of a σ B -Dependent Small Noncoding RNA in Listeria monocytogenes

Cited by 46 publications

References 36 publications

Listeria monocytogenesσBModulates PrfA-Mediated Virulence Factor Expression

Listeria monocytogenesσBModulates PrfA-Mediated Virulence Factor Expression

Survival of Listeria monocytogenes in Soil Requires AgrA-Mediated Regulation

CodY Activates Transcription of a Small RNA inBacillus subtilis

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Identification of a σ ^B -Dependent Small Noncoding RNA in Listeria monocytogenes

Listeria monocytogenesσ^BModulates PrfA-Mediated Virulence Factor Expression

Listeria monocytogenesσ^BModulates PrfA-Mediated Virulence Factor Expression