Identification of aberrantly expressed of serum microRNAs in patients with hormone-induced non-traumatic osteonecrosis of the femoral head

Wei, Biaofang; Wei, Wei

doi:10.1016/j.biopha.2015.07.016

Cited by 25 publications

(19 citation statements)

References 28 publications

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“…Wei et al suggested that miR-596 exerted the role in glioma invasion via regulating CREPT [46]. In patients with SANFH, miR microarray analysis showed that miR-596 level in plasma was significantly elevated [18]. However, the function of miR-596 in SANFH remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

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MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

Liu

Lei

2020

J Orthop Surg Res

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Background: It is reported that miR-596 has a potential diagnostic value for non-traumatic osteonecrosis of the femoral head (NOFH), but its underlying mechanisms in NOFH is unclear. Methods: The expression of miR-596 and Smad3 was detected by western blot and quantitative real-time PCR. The relationship between the two molecules was explored using Dual-Luciferase Reporter Assay. Glucocorticoid (GC)dexamethasone, was used to induce bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation, and the effects of miR-596 on BMSC osteogenic differentiation and proliferation were determined.Results: MiR-596 expression was upregulated, while Smad3 expression was inhibited in the bone marrow samples of patients with steroid-induced osteonecrosis of femoral head (SANFH). Overexpression of miR-596 inhibited the proliferation and osteogenic differentiation of BMSCs induced by GC. Meanwhile, the opposite results were observed in the miR-596 inhibitor group. In addition, Smad3 was a target gene of miR-596, and negatively regulated by miR-596. The promotion effect of the miR-596 inhibitor on BMSC proliferation and osteogenic differentiation was reversed by si-Smad3.Conclusion: MiR-596 can suppress GC-BMSC osteoblastic differentiation and proliferation by regulating Smad3 expression.

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Section: Discussionmentioning

confidence: 99%

“…MiR-596 is a kind of miRNAs, which was indispensable for the differentiation, proliferation, and migration of cancer cells [16,17]. It was reported that plasma miR-596 level was significantly increased in patients with NOFH, which had potential diagnostic value for NOFH [18]. However, the underlying mechanisms of miR-596 in SANFH are unclear.…”

mentioning

confidence: 99%

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

Liu

Lei

2020

J Orthop Surg Res

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“…miR-423-5p is downregulated in osteosarcoma cells expressing CD117 and STRO-1 (21). The miR-423-5p levels were considerably elevated and correlated with the prognosis of patients with hormone-induced non-traumatic osteonecrosis of the femoral head (27). These studies indicate that miR-148b-3p, miR-337-5p and miR-423-5p participate in osteoclast metabolism.…”

Section: Discussionmentioning

confidence: 70%

miR‑148b‑3p, miR‑337‑5p and miR‑423‑5p expression in alveolar ridge atrophy and their roles in the proliferation and apoptosis of OMMSCs

et al. 2018

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MicroRNAs (miRNAs/miRs) have key roles in various physiological and pathological processes by regulating the expression of specific genes. The identification of miRNAs involved in bone metabolism may provide insight into the expression of genes associated with the development of alveolar ridge atrophy. In the present study, the miRNA expression profiles in alveolar ridge atrophy and normal tissue samples were investigated by miRNA microarray analysis. Among the 52 differentially expressed miRNAs identified, the expression levels of 20 selected miRNAs in the alveolar ridge atrophy and normal tissue samples were verified by reverse transcription-quantitative polymerase chain reaction. The results indicated that the expression levels of 11 miRNAs were significantly different between alveolar ridge atrophy and normal tissue samples; however, only three of them (miR-148b-3p, miR-337-5p and miR-423-5p) were previously reported to be involved in bone metabolism. In vitro, miR-148b-3p, miR-337-5p and miR-423-5p mimics promoted the proliferation and inhibited apoptosis of bone marrow mesenchymal stem cells from orofacial bone (OMMSCs), while antisense inhibitors of these miRNAs had the opposite effect. In conclusion, the present study indicated that these miRNAs are involved in the pathogenesis of alveolar ridge atrophy. miR-148b-3p, miR-337-5p and miR-423-5p promote the proliferation of OMMSCs and inhibit their apoptosis. The present results provide a novel perspective for understanding the pathogenesis of alveolar ridge atrophy.

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“…ey further revealed that the regulatory effect of miR-1207-5p on steroid-induced ONFH was related to its effect on epidermal growth factor receptors using rat models. [23] used a miRNA microarray to profile miRNA expression in the serum of patients with steroid-induced ONFH and healthy volunteers in an attempt to identify new biomarkers for diagnosis. ey found three miRNAs that were downregulated and nine miRNAs that were upregulated in the serum of the ONFH patients.…”

Section: Micrornas In Steroid-induced Onfhmentioning

confidence: 99%

Noncoding RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

Sun

Tan

2019

BioMed Research International

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Steroid-induced osteonecrosis of the femoral head (ONFH) is a severe orthopedic disease caused by the long-term administration of glucocorticoids. The main pathological feature of ONFH is the gradually progressive necrosis of bone cells and the bone marrow, ultimately resulting in structural changes or even complete collapse of the femoral head. However, the exact pathogenic mechanism of ONFH remains unknown. Noncoding RNAs (ncRNAs) have emerged as very powerful regulators of gene expression, functioning at both transcriptional and posttranscriptional levels in the pathogenesis of ONFH. Here, we review the current knowledge of the role of ncRNAs, including microRNAs, long noncoding RNAs, and circular RNAs, in the pathogenesis of steroid-induced ONFH. Further focus and validation of these associations can provide new insight into the pathogenic mechanisms at the molecular level to suggest targets for treatment and prevention.

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Identification of aberrantly expressed of serum microRNAs in patients with hormone-induced non-traumatic osteonecrosis of the femoral head

Abstract: We report a miRNA profile of hormone-NOFH and provide a new perspective to understand this intricate disease. This novel information suggests the potential roles of miR-423-5p in the diagnosis, prognosis biomarkers, or therapy targets of hormone-NOFH.

Cited by 25 publications

References 28 publications

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

miR‑148b‑3p, miR‑337‑5p and miR‑423‑5p expression in alveolar ridge atrophy and their roles in the proliferation and apoptosis of OMMSCs

Noncoding RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

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