Identification of acetogenin compound, sabadelin from soursop seeds, Annona muricata (Annonaceae)

Abstract

Isolation and identification of one chemical compound from the seeds of soursop, Annona muricata (Annonaceae) based on bioactivity-guided fractionation by the Brine Shrimp Lethality Assay (BSLA) have been done. The powder of soursop seed was extracted successively with n-hexane, chloroform, and methanol then the methanol extract was partitioned with ethylacetate water. Isolation and purification of ethylacetate extracts were carried out by column chromatography [(SiO2; i. n-hexane-chloroform = 15:1 ~ 1:1 gave one pure isolatedcompound. Based on Ultra Violet (UV), Infra-Red (IR), Nuclear Magnetic Resonance (NMR), and mass spectra (LC-MS/MS), the isolated Fr-4 was an acetogenin compound, sabadelin which had a weak cytotoxicity LC50 of 487 ppm.