Identification of active component in red pomegranate (punica granatum L.) seeds ethanolic extract

Abstract

Objective: Pomegranate (punica granatum l.) have many benefits for health and used as traditional medicine. The pomegranate seeds are rich in antioxidants, namely flavonoids and tannins as well as alkaloid which is an antibacterial. The Aim of this study was to identify the pure isolate/active component of red pomegranate seeds ethanolic extract. Material and Methods: Two-ways TLC of the purified crystals which were obtained from the hexan fraction of ethanolic extract red pomegranate seeds, were used to make sure there was only 1 active compound of red pomegranate seeds. The isolate was identified using dragendorff, Mg and HCl, NaOH, Bunchard, Liebermen, FeCL 3 and HCl concentrated reagents. active compound of red pomegranate seeds. The isolate was identified using dragendorff, Mg and HCl, NaOH, Bunchard, Lieberme using dragendorff, Mg and HCl, NaOH, Bunchard, Liebermen, FeCL 3 and HCl concentrated reagents. Results: Isolate identification test using dragendorf reagents showed positive result (an orange-coloured). Conclusion: The pure isolate/active component of red pomegranate seeds is alkaloid.

Study of the Effect of Extraction Using Enzyme Preparation - Pectinol F-RKM 0719 on the Yield of Phenolic Substances in Pomegranate Peel

“…The pomegranate peel is rich in phenol compounds such as hydrolyzable tannins (punicalin, punicalagin, ellagic acid and gallic acid), flavonoids (anthocyanins and catechins) and other nutrients that are accountable for its biological activity [5][6][7].…”

“…After closing the vacuum, the throughput valve (6) activates the work of the vacuum pump (7). After measuring the residual pressure in the system (5) and setting the duration of the processing of raw materials with ultrasound, the low-frequency ultrasonic apparatus (3) is turned on.…”

“…Upon reaching the specified time of ultrasonic treatment of the raw materials under study, while the vacuum pump (7) is turned off and the vacuum throughput valve (6) is opened, the glass container with the extract (2) is taken out. Then the extract is filtered through a sieve and the remaining raw material is squeezed out.…”