Identification of AFLP markers closely linked to the powdery mildew resistance genes Pm1c and Pm4a in common wheat (Triticum aestivum L.)

Hartl, L.; Mohler, Volker; Zeller, F. J.; Hsam, S. L. K.; Schweizer, Günther

doi:10.1139/g98-129

Cited by 71 publications

(54 citation statements)

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“…Earlier AFLP-based marker studies have been found to be informative in assessment of genetic diversity in wheat varieties started in 1998 [70,71]. Regarding the investigation of traits associated with yield, Goodwin et al [72] and Hartl et al [73] initiated the AFLP technique to develop an AFLP marker associated with resistance to Septoria tritici blotch and powdery mildew, respectively. Hartl et al identiied several AFLP markers closely linked to the Pm1c and Pm4a [73].…”

Section: Ampliied Fragment Length Polymorphism (Aflp) Markersmentioning

confidence: 99%

“…Regarding the investigation of traits associated with yield, Goodwin et al [72] and Hartl et al [73] initiated the AFLP technique to develop an AFLP marker associated with resistance to Septoria tritici blotch and powdery mildew, respectively. Hartl et al identiied several AFLP markers closely linked to the Pm1c and Pm4a [73]. Out of 92 AFLP primer combinations, 31 polymorphic fragments detected between the resistance and susceptible lines, of which eight were found to be the most reliable polymorphic markers.…”

Section: Ampliied Fragment Length Polymorphism (Aflp) Markersmentioning

confidence: 99%

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Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Kim¹,

Kim²,

Jang³

2017

Wheat Improvement, Management and Utilization

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This chapter addresses the development and use of molecular markers for yield enhancement in wheat. Since their key goal for breeding is to maximize yield, extensive eforts have been made toward the improvement of yield. Agronomic traits related to yield, yield-related, disease resistance, and abiotic stresses are considered to be quantitative traits (QTLs), also known as complex traits, because they are controlled by numerous genes and are afected by environmental factors. Researchers have been studying such traits in the past decades for the development of molecular markers which can be used in various wheat breeding studies mainly involving restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), single nucleotide polymorphism (SNP), random ampliied polymorphic DNA (RAPD), and ampliied fragment length polymorphism (AFLP). Furthermore, the advent of next-generation sequencing (NGS) has accelerated the discovery of agronomically important genes. All of the technologies have enabled great advances for increasing the productivity of wheat. Here, the past history of irst-generation sequencing, present status of second-generation sequencing, and future potential of translational genomics linked to the yield will be discussed.

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Section: Ampliied Fragment Length Polymorphism (Aflp) Markersmentioning

confidence: 99%

Section: Ampliied Fragment Length Polymorphism (Aflp) Markersmentioning

confidence: 99%

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Kim¹,

Kim²,

Jang³

2017

Wheat Improvement, Management and Utilization

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show abstract

“…DNA quantity and quality were assessed with a UV-Photometer. The AFLP analysis was performed in a similar way to that described by Hartl et al (1999) using the enzyme combination Sse8387I and MseI. Thirty Sse8387I+NN/MseI+NN primer combinations with two selective nucleotides on the 3 ‫׳‬ -end of both primers were used for selective PCR amplification.…”

Section: Genomic Dna Extraction Aflp and Ssr Markersmentioning

confidence: 99%

Specific Resistance Genes in Wheat Chinese Landrace ‘Wangshuibai’ against Two Iranian Mycosphaerella graminicola Isolates

Talebi¹,

Mardi

Jelodar³

et al. 2010

IJB

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Septoria tritici blotch, caused by the fungus Mycosphaerella graminicola, is currently the major foliar disease of wheat world-wide, and new sources of resistance and knowledge about the genetics of resistance are needed to improve breeding for resistance against this disease. An F 10 recombinant inbred population from a cross between 'Wangshuibai' (Chinese landrace) and the susceptible cultivar 'Seri82' was tested at seedling stage under controlled greenhouse conditions. Two isolate-specific genes for resistance to Iranian M.graminicola isolates IPO08002 and IPO08003 were detected on Chromosomes 2BL and 7DS, respectively. For both genes the resistance was derived from 'Wangshuibai' at positions where Stb9 (on 2BL) and Stb4 (on 7DS) have been

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“…High reproducibility, rapid generation, and high frequency of identifiable polymorphisms make the amplified fragment length polymorphism (AFLP) analysis (Vos Pieter et al, 1995) an attractive technique for indentifying polymorphic markers linked to important traits by analyzing individuals from segregating populations (Goodwin et al, 1998;Hartl et al, 1999). However, AFLP analysis is tedious and time-consuming since it involves several steps: DNA digestion, ligation, preamplification, and selective amplification.…”

Section: Introductionmentioning

confidence: 99%

Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa)

Wang¹,

Bi²,

Yuan³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. To identify amplified fragment length polymorphism (AFLP) markers associated with resistance or susceptibility of alfalfa to common leafspot (CLS) caused by the fungus Pseudopeziza medicaginis (Dermateaceae), bulked segregant analysis was conducted based on an F 1(M × M) population of 93 plants and a BC 1 S population of 91 plants. Three AFLP markers, ACTCAA R206 , TAGCAC R185 , and GGACTA S264 , were found to be associated with CLS resistance or susceptibility. All three markers were found at significantly different frequencies (71.9, 80.3 and 91.8%) compared to resistant or susceptible plants in the original population. Subsequently, these three AFLP markers were converted into three SCAR markers, ACTCAA R136 , TAGCAC R128 and GGACTA S254 , which are easier to employ in breeding programs. The three SCAR markers were used in a randomly selected population with 50% resistance; the probability of finding one resistant plant was increased to 67.3, 66.7 and 90.0% with markers ACTCAA R136 , TAGCAC R128 and GGACTA S254 , independently. If two of the SCAR markers were used simultaneously, the probability would be higher than 89%. The three SCAR markers identified in this study would be applicable for selection for CLS resistance in alfalfa breeding programs. Moreover, the genetic analysis indicated that CLS resistance in alfalfa is conferred by a single dominant gene.

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Identification of AFLP markers closely linked to the powdery mildew resistance genes Pm1c and Pm4a in common wheat (Triticum aestivum L.)

Cited by 71 publications

References 0 publications

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Past, Present and Future Molecular Approaches to Improve Yield in Wheat

Specific Resistance Genes in Wheat Chinese Landrace ‘Wangshuibai’ against Two Iranian Mycosphaerella graminicola Isolates

Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa)

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