Identification of allele specific AFLP markers linked with bacterial wilt [Ralstonia solanacearum (Smith) Yabuuchi et al.] resistance in hot peppers (Capsicum annuum L.)

Identification of a Major QTL (qRRs-10.1) That Confers Resistance to Ralstonia solanacearum in Pepper (Capsicum annuum) Using SLAF-BSA and QTL Mapping

“…ID10-194305124 had the highest LOD score and phenotypic variation R 2 among markers at the qRRs-10.1 locus (Table 3), demonstrating its potential use for MAS of BW resistance with BVRC 1 as the resistant donor parent. The major BW resistance QTL qRRs-10.1 identified in the present study differs from those identified in previous studies that suggested the existence of disease resistance loci on chromosomes 1 and 11 [17,18]. This can be explained by the use of different parents, isolates, inoculation methods, scoring methods, and population types.…”

“…Several sources of genetic resistance to BW have been identified in pepper to date. However, the genetic basis of BW resistance in these pepper lines remains elusive and is even controversial as different sources have different inheritance mechanisms [10,12,13,14,15,16,18]. In this study, comparison of the DSI values of F 1 , BC 1 P s , and BC 1 P r with those of the parents indicated that BW resistance is a partially dominant trait (Table 1).…”

“…Similarly, in previous studies, the disease severity in F 1 was similar to or less than the mean mid-parent value [10,16], indicating that BW resistance in pepper generally is partially dominant. Conversely, Thakur et al [18] studied the inheritance of BW resistance in an F 2 population derived from two pepper near-isogenic lines, Pusa Jwala and Anugraha, which differ only in BW resistance, and found that the resistance was governed by homozygous recessive gene action. These discrepancies may be due to various factors, such as the use of different breeding lines, isolates, and the criteria used for scoring.…”

“…Diverse modes of gene interaction that depend on the virulence level of R. solanacearum isolates have been reported in peppers, indicating the complexity of the inheritance of BW resistance [10]. Molecular genetic analysis of an F 2 population constructed from near-isogenic lines (Anugraha and Pusa Jwala) revealed that BW resistance is recessive, and polymorphic AFLP marker ( Eco ACT + Mse CAC) bands (103, 117, and 161 bp) associated with the recessive resistant allele have been identified [18]. The tomato cultivar Hawaii 7996 has stable resistance to R. solanacearum , and is intensively used for BW resistance QTL mapping.…”

Analysis and feasibility study of plant disease using e-nose

“…However, further study by few researchers reported that the bacteria causing this disease typed to the genus of Erwinia sp. [1 [8][9][10][11][12][13][14][15][16][17][18][19][20][21]. The usual symptoms of this disease include blackish, mushy, water-soaked lesions on leaves, while firm, water-soaked cankers develop on the stem, which leads to the destruction of papaya trees most of the bacteria infected fruits shows symptoms with dark spots on the fruit's skins and flesh with water-soaked area [21 ] [22].…”