Identification of Allergenic Component Tyr p 8 from Tyrophagus putrescentiae and Cross-Reactivity with Der p 8

Abstract

Group 8 mite allergens exhibit sequence homology to glutathione S-transferases (GSTs), such as that from Dermatophagoides pteronyssinus (Der p 8). GSTs have been identified as important allergens in studies of allergens from house dust mites, cockroaches, and fungi. Our objective was to purify the native group 8 allergen from Tyrophagus putrescentiae (nTyr p 8) and generate recombinant Tyr p 8 (rTyr p 8) for immunological characterization. The allergenicity was determined by antibody recognition, IgE inhibition, and triggering of the basophil-sensitized release of histamine, using T. putrescentiae hypersensitivity sera. The results showed that the mRNA transcript of nTyr p 8 is 657 bp long, contains 218 amino acids with a molecular mass of 26 kDa, and exhibits 83% sequence homology to Der p 8. Serum samples from the allergic patients with an IgE-positive response to T. putrescentiae were analyzed to determine their IgE response to rTyr p 8. The results showed that the sera of 48 subjects (45.3%) had specific IgE against rTyr p 8. However, sera of only 19 subjects (17.9%) had specific IgE against rTyr p 8 after D. pteronyssinus absorption. Histamine release was observed from T. putrescentiae-allergic subjects in the presence of rTyr p 8. Both the nTyr p 8 and T. putrescentiae crude extract had been demonstrated to possess GST enzymatic activity. Although the specific binding of serum IgE to rTyr p 8 was only 17.9%, which indicates that rTyr p 8 was not a major allergen, the positive response to rTyr p 8 was due to the cross-reactivity with Der p 8. The group 8 mite allergen might be of use in the design of a suitable allergen for diagnosis and for the development of novel immunotherapies.

Analysis of glutathione S-transferase allergen cross-reactivity in a North American population: Relevance for molecular diagnosis

“…In contrast, two different classes of GST from Blattella germanica (mu Bla g 5 and a delta GST, sharing only 14% identity), showed IgE reactivity that was attributed to co-sensitization. 29,30 Despite this general rule, an unexpected cross-reactivity between Bla g 5 and a GST from Wuchereria bancrofti (WbGST), a major lymphatic filarial pathogen of humans, was reported, with only a 27.9% identity between both proteins (alignment in Fig. E1, Online Repository).…”

Cross-reactivity between group-5 and -21 mite allergens from Dermatophagoides farinae, Tyrophagus putrescentiae and Blomia tropicalis

“…Five Tyrophagus putrescentiae allergens have been described to date: Tyr p 2, Tyr p 3, Tyr p 8, Tyr p 10 and Tyr p 24 (16). Of these, Tyr p 2, Tyr p 8 and Tyr p 10 have been shown to cause cross-reactivity (6,11,15,17).…”

“…For example, tropomyosin, a group-10 allergen, is a well-known pan-allergen that causes cross-reactivity among mites, a variety of invertebrates (5,6) including shrimps and crabs, and parasites, including Anisakis nematodes and roundworms (7)(8)(9). Group-8 mite allergens (glutathione S-transferase) have also been shown to be cross-reactive with other arthropod allergens (10,11). The group-2 allergens are cross-reactive among mite species (12,13) and are the primary allergens responsible for the cross-reactivity between HDM and SM in Korea (14).…”

Glutathione-S-Transferase: A Minor Allergen in Birch Pollen due to Limited Release from Hydrated Pollen

“…Several minor allergens in BP are panallergens cross-reacting with orthologs in various allergen sources. Cross-reactivity of GST from different mite species [15] , [31] and fungal sources [22] , as well as of GST from mites and cockroach [21] and recently also from cockroach and helminths has been demonstrated [23] . Therefore, we evaluated the possible cross-reactivity of bGST with HDM GST Der p 8, a prominent cross-reactive allergen [14] , [26] .…”