Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS

Chebib, Soraya; Meng, Chen; Ludwig, Christina; Bergmann, Karl‐Christian; Becker, Sylvia; Dierend, Werner; Schwab, Wilfried

doi:10.1016/j.fochms.2022.100111

Cited by 8 publications

(11 citation statements)

References 46 publications

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“…A different allergenic potential is discussed for specific isoallergens, modulating the allergenic potential of a variety, but so far only few and contradicting results with regard to the effects of isoallergens on the allergenicity are reported in the literature. The non-targeted approach of Chebib et al indicated a remarkable effect of 1.03 on the allergenic potential; however, the data reported are based on the abundance of peptides for specific isoforms . The correlation of allergenicity with the sum of the content of 1.03 and 1.06 supported the assumption of an increased allergenic potential of these minor isoallergens.…”

Section: Discussionmentioning

confidence: 99%

“…6 However, so far, the quantification of Mal d 1 has been carried out exclusively with immunological methods 11,14−16 or gene expression studies 6,14,17 or as relative quantification using mass spectrometry. 18 These methods do not allow a reliable absolute isoallergen-specific quantification. To obtain profounder knowledge on the isoallergen profile of Mal d 1 in apples, a method for an isoallergen-specific absolute quantification, based on isotope dilution analysis, was developed for freeze-dried apple samples using a mass spectrometric bottom-up approach.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Besides the impact of the polyphenol content and profile, , an influence of the Mal d 1 isoallergen profile on the allergenic potential is discussed . However, so far, the quantification of Mal d 1 has been carried out exclusively with immunological methods ,− or gene expression studies ,, or as relative quantification using mass spectrometry . These methods do not allow a reliable absolute isoallergen-specific quantification.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Quantification of Mal d 1 Isoallergen Profiles and Contents in Traditional and Commercial Apple Varieties by Mass Spectrometry

Kaeswurm

Straub

Siegele³

et al. 2023

J. Agric. Food Chem.

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The apple allergy in Northern Europe is a cross-reaction to the birch pollen allergy. No correlation between the allergenicity of an apple variety and the content of the major apple allergen Mal d 1, a homologue to the Bet v 1 allergen in birch, could be found using ELISA, so far. Therefore, an impact of polyphenols and/or differences in the isoallergen profile are discussed. To allow a more detailed analysis of the Mal d 1 content and the isoallergen profile, a mass spectrometric method was applied to investigate differences in the flesh and peel of 10 traditional varieties and 10 commercial breeds. The data revealed often, but not always, lower Mal d 1 contents in traditional varieties grown in orchard meadows, which was more obvious in the flesh. Differences among the peels were less pronounced. A closer look at the individual isoallergens 1.01, 1.02, 1.03, and 1.06 reveals an increased impact of the minor isoallergens 1.03 and 1.06 on the allergenic potential, since commercial breeds like Braeburn, Santana, and Holstein Cox, which are considered to have reduced allergenic potentials, were characterized by low levels of these isoallergens.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization and Quantification of Mal d 1 Isoallergen Profiles and Contents in Traditional and Commercial Apple Varieties by Mass Spectrometry

Kaeswurm

Straub

Siegele³

et al. 2023

J. Agric. Food Chem.

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“…Genes related to these proteins have been recently demonstrated being differentially expressed when fruits from non-Italian D. kaki cultivars were compared ( Zheng et al., 2021 ; Kou et al., 2021b ). Along with glyceraldehyde 3-phosphate dehydrogenase, profilin and isoflavone reductase, some thaumatin-like proteins, Bet v1-like protein and endochitinases are well-known allergenic components present in persimmon and other fruits ( ) ( Karamloo et al., 2001 ; Bolhaar et al., 2005 ; Ballmer-Weber and Hoffmann-Sommergruber, 2011 ; Tsai et al., 2017 ; Chebib et al., 2022 ). Figure 4 summarizes the relative representation of allergenic proteins in fruits of the persimmon accessions here investigated, with possible implications on the allergic impact of corresponding pomes.…”

Section: Resultsmentioning

confidence: 99%

“…Hoffmann-Sommergruber, 2011; Tsai et al, 2017;Chebib et al, 2022). Figure 4 summarizes the relative representation of allergenic proteins in fruits of the persimmon accessions here investigated, with possible implications on the allergic impact of corresponding pomes.…”

Section: Proteomic Analysismentioning

confidence: 99%

Investigating phenotypic relationships in persimmon accessions through integrated proteomic and metabolomic analysis of corresponding fruits

et al. 2023

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Together with phenological and genomic approaches, gel-based and label-free proteomic as well metabolomic procedures were separately applied to plants to highlight differences between ecotypes, to estimate genetic variability within/between organism populations, or to characterize specific mutants/genetically modified lines at metabolic level. To investigate the possible use of tandem mass tag (TMT)-based quantitative proteomics in the above-mentioned contexts and based on the absence of combined proteo-metabolomic studies on Diospyros kaki cultivars, we here applied integrated proteomic and metabolomic approaches to fruits from Italian persimmon ecotypes with the aim to characterize plant phenotypic diversity at molecular level. We identified 2255 proteins in fruits, assigning 102 differentially represented components between cultivars, including some related to pomological, nutritional and allergenic characteristics. Thirty-three polyphenols were also identified and quantified, which belong to hydroxybenzoic acid, flavanol, hydroxycinnamic acid, flavonol, flavanone and dihydrochalcone sub-classes. Heat-map representation of quantitative proteomic and metabolomic results highlighted compound representation differences in various accessions, whose elaboration through Euclidean distance functions and other linkage methods defined dendrograms establishing phenotypic relationships between cultivars. Principal component analysis of proteomic and metabolomic data provided clear information on phenotypic differences/similarities between persimmon accessions. Coherent cultivar association results were observed between proteomic and metabolomic data, emphasizing the utility of integrating combined omic approaches to identify and validate phenotypic relationships between ecotypes, and to estimate corresponding variability and distance. Accordingly, this study describes an original, combined approach to outline phenotypic signatures in persimmon cultivars, which may be used for a further characterization of other ecotypes of the same species and an improved description of nutritional characteristics of corresponding fruits.

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In silico Identification of Sequential Similarities of Selected Lipid Transfer Proteins

Hovaňáková,

Klongová,

Žiarovská

2024

Agrobiodivers Improv Nutr health life Qual

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Nonspecific lipid transfer proteins (nsLTP) are common allergens discovered and described 40 years ago. They are present in many plant species and protect plants from stressors such as heat and drought. Studies on the peach nsLTP, Pru p 3, show that nsLTP are very cross-reactive through presented IgE epitopes shared by nsLTP from other botanically related fruits. These allergens are to varying degrees resistant to heat and digestion, and sensitization may occur through the oral, cutaneous, or inhaled routes. In the last years, several web tools for the prediction of allergenicity of new molecules based on their homology with known allergens have been created, and have established guidelines to assess potential allergenicity of proteins through bioinformatics. Here, Allerbase and NCBI Protein BLAST were applied to characterize the protein sequences of nsLTPs of plant species -Malus domestica Borkh., Prunus persica (L.) Batsch., Daucus carota (L.), Vitis vinifera (L.) and Solanum lycopersicum (L.). Obtained primary data were evaluated by the algorithm NCBI BLAST (Basic Local Alignment Search Tools). This tool allows searching similar protein sequences, in this case, was used Protein BLAST for comparing protein sequences. Most of the analysed sequences displayed a high probability of being allergenic according to the high sequencing identity and sharing IgE epitopes to each other. The highest sequencing identity in preselected plant species was presented between Pru p 3 (isoform Pru p 3.0102) and Mal d 3 (isoform Mal d 3.0101), 91.32% and they share the same epitope, NNA. The lowest sequencing homology was identified between Mal d 3.0203 and Dau c LTP, 43.10% and they did not share any epitope to each other.

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Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS

Cited by 8 publications

References 46 publications

Characterization and Quantification of Mal d 1 Isoallergen Profiles and Contents in Traditional and Commercial Apple Varieties by Mass Spectrometry

Characterization and Quantification of Mal d 1 Isoallergen Profiles and Contents in Traditional and Commercial Apple Varieties by Mass Spectrometry

Investigating phenotypic relationships in persimmon accessions through integrated proteomic and metabolomic analysis of corresponding fruits

In silico Identification of Sequential Similarities of Selected Lipid Transfer Proteins

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