Identification of an allergenic calmodulin from Amaranthus palmeri pollen

Gómez-Esquivel, Mónica Luz; Guidos, Guillermo; Rojo-Gutiérrez, María Isabel; Mellado-Abrego, Jaime; Bermejo-Guevara, Mario Alberto; Castillo-Narváez, Gloria; Velázquez-Sámano, Guillermo; Velasco-Medina, Andrea Aída; Moya-Almonte, Margaret Gissett; Vallejos-Pereira, Carla Marcela; López-Hidalgo, Marisol; Godínez‐Victoria, Marycarmen; Reyes-López, César A.

doi:10.1016/j.molimm.2021.01.031

Cited by 5 publications

(5 citation statements)

References 31 publications

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“…In C. majus latex, we have identified proteins with potential allergenic activity, such as polyphenol oxidase, which in eggplant acts as an allergen, and calmodulin-like proteins. Additionally, MLPs, which are highly representative proteins of C. majus latex, belong to the Bet v 1 superfamily of protein allergens ( Table S2 ) [ 104 , 105 , 106 ].…”

Section: Discussionmentioning

confidence: 99%

The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism

Musidlak¹,

Warowicka²,

Broniarczyk³

et al. 2022

IJMS

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Yellow-orange latex of Chelidonium majus L. has been used in folk medicine as a therapeutic agent against warts and other visible symptoms of human papillomavirus (HPV) infections for centuries. The observed antiviral and antitumor properties of C. majus latex are often attributed to alkaloids contained therein, but recent studies indicate that latex proteins may also play an important role in its pharmacological activities. Therefore, the aim of the study was to investigate the effect of the crude C. majus latex and its protein and alkaloid-rich fractions on different stages of the HPV replication cycle. The results showed that the latex components, such as alkaloids and proteins, decrease HPV infectivity and inhibit the expression of viral oncogenes (E6, E7) on mRNA and protein levels. However, the crude latex and its fractions do not affect the stability of structural proteins in HPV pseudovirions and they do not inhibit the virus from attaching to the cell surface. In addition, the protein fraction causes increased TNFα secretion, which may indicate the induction of an inflammatory response. These findings indicate that the antiviral properties of C. majus latex arise both from alkaloids and proteins contained therein, acting on different stages of the viral replication cycle.

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Section: Discussionmentioning

confidence: 99%

The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism

Musidlak¹,

Warowicka²,

Broniarczyk³

et al. 2022

IJMS

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“…Calmodulin was revealed as an allergen of Amaranthus palmeri pollen, which is a valuable source of pollinosis in North America, Asia and Europe. 43…”

Section: Resultsmentioning

confidence: 99%

“…42 Calmodulin was revealed as an allergen of Amaranthus palmeri pollen, which is a valuable source of pollinosis in North America, Asia and Europe. 43 Lysozyme, a glycosidase, is one of the egg white proteins that has been reported as a major allergen. 44 The proteins, identified in the present study can be potentially taken as markers to improve the sensitivity and specificity when testing IgE in the blood to foods involving large yellow croaker (Larimichthys crocea), swimming crab (Portunus trituberculatus) and black tiger shrimp (Penaeus monodon).…”

Section: Identification Of Allergens From the Model Seafoodsmentioning

confidence: 99%

On-chip immunomagnetic separation of allergens from myofibrillar proteins of seafoods for rapid allergy tests

Wang

2022

Analyst

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“…Hence, calmodulin is not exclusive to Artemisia but has universal homology in other plants. It has also been identified as a potential allergen in ash and Amaranthus palmeri pollen ( 19 , 20 ). About 28.57% of Artemisia allergic patients’ IgE can recognize ArtCaM, while 27.78% of other allergic patients’ IgE can recognize ArtCaM.…”

Section: Discussionmentioning

confidence: 99%

“…In animals, extracellular calmodulin is present in body fluids, saliva, urine, and milk ( 15 ) and is involved in nerve regeneration ( 16 ), vascular activity ( 17 ), embryonic development ( 18 ), and so on. Interestingly, a recent study has identified allergenic calmodulin in ash pollen and Amaranthus palmeri pollen ( 19 , 20 ), suggesting the potential role of calmodulin families as allergens.…”

Section: Introductionmentioning

confidence: 99%

An allergenic plant calmodulin from Artemisia pollen primes human DCs leads to Th2 polarization

Zhang

Hu²,

Chen³

et al. 2022

Front. Immunol.

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Artemisia pollen is the major cause of seasonal allergic respiratory diseases in the northern hemisphere. About 28.57% of Artemisia allergic patients’ IgE can recognize ArtCaM, a novel allergenic calmodulin from Artemisia identified in this study. These patients exhibited stronger allergic reactions and a longer duration of allergic symptoms. However, the signaling mechanism that triggers these allergic reactions is not fully understood. In this study, we found that extracellular ArtCaM directly induces the maturation of human dendritic cells (DCs), which is attributed to a series of Ca2+ relevant cascades, including Ca2+/NFAT/CaMKs. ArtCaM alone induces inflammatory response toward Th1, Th17, and Treg. Interestingly, a combination of ArtCaM and anti-ArtCaM IgE led to Th2 polarization. The putative mechanism is that anti-ArtCaM IgE partially blocks the ArtCaM-induced ERK signal, but does not affect Ca2+-dependent cascades. The crosstalk between ERK and Ca2+ signal primes DCs maturation and Th2 polarization. In summary, ArtCaM related to clinical symptoms when combined with anti-ArtCaM IgE, could be a novel allergen to activate DCs and promote Th2 polarization. Such findings provide mechanistic insights into Th2 polarization in allergic sensitization and pave the way for novel preventive and therapeutic strategies for efficient management of such pollen allergic disease.

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Identification of an allergenic calmodulin from Amaranthus palmeri pollen

Cited by 5 publications

References 31 publications

The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism

The Activity of Chelidonium majus L. Latex and Its Components on HPV Reveal Insights into the Antiviral Molecular Mechanism

On-chip immunomagnetic separation of allergens from myofibrillar proteins of seafoods for rapid allergy tests

An allergenic plant calmodulin from Artemisia pollen primes human DCs leads to Th2 polarization

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