Identification of an arthropod molecular target for plant-derived natural repellents

Tian, Quan; Wang, Peiyu; Xie, Chang; Pang, Peiyuan; Zhang, Youjing; Gao, Yue; Cao, Zhijian; Wu, Yingliang; Li, Wenxin; Zhu, Michael X.; Li, Dongdong; Yao, Jing

doi:10.1073/pnas.2118152119

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…We describe the antennae-expressed TcTRPA1-mediated repellency behavior by which T. castaneum responds to citronellal, our results suggest that coleopteran TRPA1 represents a potential target for sustainable insect repellents. Intriguingly, Tian et al recently reported that D. melanogaster TRPγ, contained in TRPC subfamily, was directly activated by citronellal to initiate repellent behavior 38 , for which further experiments and screening are desirable even in coleopteran beetles.…”

Section: Discussionmentioning

confidence: 99%

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

Shimomura

Ino

Tamura

et al. 2022

Sci Rep

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The sensory perception of irritant chemicals results in escape and repellency behavioral patterns in insects. Transient receptor potential channels are cation channels that function as sensor proteins for several types of signals, such as light, sound, temperature, taste, as well as chemical and physical stimuli; among these, the TRPA channel is widely conserved and activated by irritant chemicals. Certain plant-derived essential oils (EOs), produced by secondary metabolism, are mixtures of volatile compounds, which are used as repellents because they contain environmentally sustainable ingredients. Citronellal, which is present in citronella EO from Cymbopogon species, is a potentially viable insect repellent; however, the repellency capability against coleopteran beetles remains limited. We investigated the citronellal-derived repellency behavior for the red flour beetle Tribolium castaneum, in which TcTRPA1 and odorant receptor co-receptor (Orco) expressions were mediated by RNA interference. Area-preference tests showed dose-dependent repellency behavior for citronellal; additionally, both TcTRPA1 and TcOrco double-strand RNA (dsRNA) micro-injection showed clear TcTRPA1 and TcOrco transcript reductions, and only TcTRPA1 dsRNA treatment significantly impaired repellency behavior. The relative expression level of the TcTRPA1 transcripts, evaluated by quantitative reverse-transcription PCR (qRT-PCR), revealed dominant expression in the antennae, indicating the antennae-expressed TcTRPA1-mediated repellency behavior.

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Section: Discussionmentioning

confidence: 99%

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

Shimomura

Ino

Tamura

et al. 2022

Sci Rep

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“…Like 2MT, other TRP channel activators drive multiple sensory pathways in insects. Flies detect naturally occurring insect repellent citronellal through ORs, TrpA1 expressed in OSNs and taste neurons, and Trpγ in OSNs (Kwon et al, 2010;Du et al, 2015;Tian et al, 2022). The repellent effect of camphor is mediated by Trpl expressed in taste neurons and ORs (Zhang et al, 2013;Wang Q. et al, 2021), and the repulsion caused by menthol involves TrpA1 and ORs (Boonen et al, 2021;Wang Q. et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Among them, TRP channels play a key role in nocifensive behavior and aversive responses to various chemical stimuli in many insect species. For example, TRPC channels are activated by carbon dioxide, and the natural repellents, camphor, citronellal, and citronellol in the fruit fly, Drosophila melanogaster (Badsha et al, 2012;Zhang et al, 2013;Tian et al, 2022). A TRPM channel is activated by menthol in the red flour beetle, Tribolium castaneum (Shimomura et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Avoidance of thiazoline compound depends on multiple sensory pathways mediated by TrpA1 and ORs in Drosophila

Sato,

Magaji,

Tominaga

et al. 2023

Front. Mol. Neurosci.

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Transient receptor potential (TRP) channels are primary sensory molecules in animals and are involved in detecting a diverse range of physical and chemical cues in the environments. Considering the crucial role of TRPA1 channels in nocifensive behaviors and aversive responses across various insect species, activators of TRPA1 are promising candidates for insect pest control. In this study, we demonstrate that 2-methylthiazoline (2MT), an artificial volatile thiazoline compound originally identified as a stimulant for mouse TRPA1, can be utilized as a novel repellent for fruit flies, Drosophila melanogaster. We observed that 2MT induced strong, dose-dependent avoidance behaviors in adult males, regardless of their feeding states, as well as egg laying behavior in females. These aversive responses were mediated by contact chemosensation via TrpA1 and olfaction via odorant receptors. Knocking down TrpA1 revealed the essential roles of bitter taste neurons and nociceptive neurons in the legs and labellum. Furthermore, among five isoforms, TrpA1-C and TrpA1-D exclusively contributed to the aversiveness of 2MT. We also discovered that these isoforms were directly activated by 2MT through covalent modification of evolutionarily conserved cysteine residues. In conclusion, we have identified 2MT as a stimulant for multiple sensory pathways, triggering aversive behaviors in fruit flies. We propose that 2MT and related chemicals may serve as potential resources for developing novel insect repellents.

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“…[4] These phytochemicals rose to prominence is due to they are readily biodegradable, relatively less toxic, and less DOI: 10.1002/masy.202300005 harmful to the environment and the consumer. [5,6] A biologically active compound must have a correct hydrophilic and hydrophobic properties for optimum activity. [7] The best active lead molecules are usually too lipophilic nor hydrophilic.…”

Section: Introductionmentioning

confidence: 99%

Microwave‐Mediated Modification of Selected Limonoids with DMAB Reagent and Antibacterial Studies

Jayakumar,

Saxena,

Tomar

2024

Macromolecular Symposia

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N,N‐Dimethylaniline.borane (DMAB) is a stable, multifaceted electrophilic boron agent that displays hydroboration of C = C as well as selective reduction of functional groups under reflux, microwave, and ultrasound conditions. Natural products contain a plethora of functional groups that govern the specific bioactivity. It will be interesting to correlate the change in a functional group with the concern for change in bioactivity. Current research emphasizes the influence of microwave irradiation on moderately reactive DMAB reagent towards the functional group modification of selected limonoid‐phytochemicals of Meliaceae family viz. cedrelone, azadirone, nimbin, and azadiradione. DMAB conveniently reduces the ketone, epoxide group into alcohol, and does a hydroboration reaction on the C = C bond. It does not show interest to reduce the ester group irrespective of 30 min of MW irradiation. Except for these reducing properties, the reagent DMAB unveils chemo‐selective nature in respect of different functional groups and utilizes in an asymmetric synthesis. The coalescence of amine.borane with microwave irradiation brings forth a commodious and prompt method for the selective reduction of functional groups. The bioactive efficacy of modified phytochemicals is further taken for the antimicrobial studies.

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Identification of an arthropod molecular target for plant-derived natural repellents

Cited by 8 publications

References 42 publications

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

TRPA1-mediated repellency behavior in the red flour beetle Tribolium castaneum

Avoidance of thiazoline compound depends on multiple sensory pathways mediated by TrpA1 and ORs in Drosophila

Microwave‐Mediated Modification of Selected Limonoids with DMAB Reagent and Antibacterial Studies

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