Identification of an epidemic strain ofAcinetobacter baumanii using electrophoretic typing methods

Matar, Ghassan M.; Cooksey, Robert C.; Elliott, John A.; Heneine, Walid; Uwaydah, Marwan; Matossian, R. M.; Tenover, Fred C.

doi:10.1007/bf02427385

Cited by 10 publications

(13 citation statements)

References 30 publications

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“…It can be postulated that probably these isolates belong to the same clone of cells bu:: different conditions lead to the expression or suppiession of one or two proteins. This observation is consistent with a study on A c i n e t o b a c t e r baumam~:ii which showed that the 3 protein patterns obtained in the typing of 36 isolates, differed from each other by only one major band [15]. We have further typed ten isolates recovered from an orange juice outbreak in USA in an attempt to assess the utility of the method in epidemiological studies.…”

Section: Discussionsupporting

confidence: 74%

“…This assay proved to be discriminatory for a variety of bacteria including H a e m o p h i l u s influenzae, Salmonella typhi and A c i n e t o b a c t e r b a u m a n i i [5,8,15]. The current study was thus undertaken to assess the subtyping potential of SDS-PAGE of total cell proteins in conjunction with AP-PCR.…”

Section: Discussionmentioning

confidence: 99%

“…Twenty-five gl of lx treatment buffer was added to all unused wells. After electrophoresis (35mA/gel, 10 °C, 3.5-5 hours), the gels were fixed and stained as previously described [15]. Total cell protein patterns were compared visually.…”

Section: Thuringiensismentioning

confidence: 99%

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Subtyping of Bacillus cereus by total cell protein patterns and arbitrary primer polymerase chain reaction

1996

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Bacillus cereus is a ubiquitous sporeforming Gram-positive rod that is associated with foodborne outbreaks as well as several opportunistic infections. Inspite of the prevalence of B. cereus associated foodborne outbreaks, subtyping of the species using molecular typing assays was not attempted. In this study we have recovered 58 B. cereus isolates from natural and clinical sources and initially characterized them, along with a B. cereus strain (ATCC 14579) and B. thuringiensis natural isolate, by biotyping, antibiotic susceptibility testing, and SDS-PAGE of total cell proteins. Our data have shown the existence of 1 biotype, 3 anti-biograms and 22 (38%) total cell protein patterns among the 58 B. cereus isolates. B. thuringiensis had a different protein pattern. SDS-PAGE of total cell proteins data denote clonal heterogeneity within B. cereus. Protein pattern 4 (pp4) was the most predominant with 13 isolates of B. cereus showing this pattern. Eight out of the 13 isolates with pp4 and one B. cereus strain (ATCC 14579) were further subtyped by using the arbitrary primer polymerase chain reaction (AP-PCR) assay. Eight (88.8%) different PCR patterns out of the 9 B. cereus isolates were obtained. Patterns obtained by SDS-PAGE of total cell proteins and AP-PCR were reproducible. These results indicate that SDS-PAGE of total cell proteins allows the differentiation among species within Bacillus and of strains within B. cereus. The typability of the method was 100% and the simpson's discrimination index of diversity was 98%. The utility of SDS-PAGE of total cell proteins in a pilot epidemiologic study was assessed and results obtained demonstrate its typing potential. AP-PCR allows further subtyping of the species. Both methods if used in conjunction may be useful for further clinical and epidemiologic studies of the spectrum of diseases caused by B. cereus.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Subtyping of Bacillus cereus by total cell protein patterns and arbitrary primer polymerase chain reaction

1996

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“…"ani-[ratus") according to the old terminology (2,9,10,18). Several authors consider A. baumannii a synonym for this old designation, enforced by the manufacturers of commercial identification systems like API (3,19,21,23,24). In the first part of the present study, 37 glucose-oxidizing (var anitratus) strains were found and of these 23 belonged to the Acb complex.…”

Section: Discussionmentioning

confidence: 99%

“…Strains of the genus Acinetobacter have been incriminated in hospital infections to an increasing extent during the last decades (3,9,15,16,21,23,25). In 1986 and 1987 Bouvet & Grimont revised the taxonomy of the genus on the basis of DNA-DNA hybridization results (4) and devised some phenotypical tests to discriminate between the DNA groups arising from the hybridization study (5).…”

mentioning

confidence: 99%

Acinetobacterin Denmark: II. Molecular studies of theAcinetobacter calcoaceticus-Acinetobacter baumanniicomplex

Gerner‐Smidt

Tjernberg

1993

Apmis

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The Acinetobacter calcoaceticus‐Acinetobacter baumannii complex consists of four closely related “genospecies” or DNA groups: DNA group 1 (A. calcoaceticus), DNA group 2 (A. baumannii), DNA 3, and Tjernberg & Ursing‘s DNA group 13. Strains in this complex are so similar phenotypically that it is often impossible to identify them to the DNA group level by the use of biochemical tests. Twenty‐three Danish clinical strains from 23 patients phenotypically identified to the A. calcoaceticus‐A. baumannii complex were studied by ribotyping, plasmid profiling, and DNA/DNA hybridization. Multiple isolates were recovered from four patients. These were identical in each patient as judged by phenotype, ribotype and plasmic profile. Seventeen different ribotypes were observed among the 23 strains, and by using this method 19 out of the 23 strains could be identified to the DNA group level. Five strains were allocated to DNA group 2 (A. baumannii), eight to DNA group 3, and six to DNA group 13. These findings were confirmed by DNA/DNA hybridization. Two of the four unidentified strains were genotypically most closely related to but different from DNA groups 1 and 3. The last two strains were most closely related to DNA group 13. These four strains represent two new DNA groups within the A. calcoaceticus‐A. baumannii‐complex. One to four plasmids in the size range 2.1 kb‐ > 100 kb were detected in 13 of the strains. Nine plasmid profiles were seen, indicating the usefulness of this typing method if the strains contain plasmids. The study also indicates that ribotyping is useful both for typing and for identification purposes, and that the genetic relationship in this area are more diverse than hitherto perceived. Taxonomic reconsiderations are warranted.

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States of Resistance: nosocomial and environmental approaches to antimicrobial resistance in Lebanon

Haraoui,

Rizk,

Landecker

2024

HPLS

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Drawing on institutional historical records, interviews and student theses, this article charts the intersection of hospital acquired illness, the emergence of antimicrobial resistance (AMR), environments of armed conflict, and larger questions of social governance in the specific case of the American University of Beirut Medical Center (AUBMC) in Lebanon. Taking a methodological cue from approaches in contemporary scientific work that understand non-clinical settings as a fundamental aspect of the history and development of AMR, we treat the hospital as not just nested in a set of social and environmental contexts, but frequently housing within itself elements of social and environmental history. AMR in Lebanon differs in important ways from the settings in which global protocols for infection control or rubrics for risk factor identification for resistant nosocomial outbreaks were originally generated. While such differences are all too often depicted as failures of low and middle-income countries (LMIC) to maintain universal standards, the historical question before us is quite the reverse: how have the putatively universal rubrics of AMR and hospital infection control failed to take account of social and environmental conditions that clearly matter deeply in the evolution and spread of resistance? Focusing on conditions of war as an organized chaos in which social, environmental and clinical factors shift dramatically, on the social and political topography of patient transfer, and on a missing “meso” level of AMR surveillance between the local and global settings, we show how a multisectoral One Health approach to AMR could be enriched by an answering multisectoral methodology in history, particularly one that unsettles a canonical focus on the story of AMR in the Euro-American context.

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Identification of an epidemic strain ofAcinetobacter baumanii using electrophoretic typing methods

Cited by 10 publications

References 30 publications

Subtyping of Bacillus cereus by total cell protein patterns and arbitrary primer polymerase chain reaction

Subtyping of Bacillus cereus by total cell protein patterns and arbitrary primer polymerase chain reaction

Acinetobacterin Denmark: II. Molecular studies of theAcinetobacter calcoaceticus-Acinetobacter baumanniicomplex

States of Resistance: nosocomial and environmental approaches to antimicrobial resistance in Lebanon

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