Identification of an estrogen-binding protein in Pseudomonas aeruginosa

Rowland, Sharon S.; Falkler, William A.; Bashirelahi, Nasir

doi:10.1016/0960-0760(92)90113-w

Cited by 25 publications

(22 citation statements)

References 23 publications

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“…A major argument against this hypothesis is that QSI activity of hormones was observed at 2 mM (0.5 mg/ml) while, in serum, concentrations of hormones such as estradiol reach up to 0.4–1.6 nM (100–400 ng/ml) in healthy women and 2–18 nM during fertilizing protocols [37]. However, the debate remains still unclosed because clinical and environmental Pseudomonas isolates are known for their capacity to import, bind and biodegrade human hormones, including estrogens, via proteins and pathways that are still poorly-characterized [38]–[40]. These hormone-modifying capabilities would contribute to underestimate the QSI-efficiency of hormones in our in vitro assay.…”

Section: Discussionmentioning

confidence: 99%

At a Supra-Physiological Concentration, Human Sexual Hormones Act as Quorum-Sensing Inhibitors

et al. 2013

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N-Acylhomoserine lactone (AHL)-mediated quorum-sensing (QS) regulates virulence functions in plant and animal pathogens such as Agrobacterium tumefaciens and Pseudomonas aeruginosa. A chemolibrary of more than 3500 compounds was screened using two bacterial AHL-biosensors to identify QS-inhibitors (QSIs). The purity and structure of 15 QSIs selected through this screening were verified using HPLC MS/MS tools and their activity tested on the A. tumefaciens and P. aeruginosa bacterial models. The IC50 value of the identified QSIs ranged from 2.5 to 90 µg/ml, values that are in the same range as those reported for the previously identified QSI 4-nitropyridine-N-oxide (IC50 24 µg/ml). Under the tested culture conditions, most of the identified QSIs did not exhibit bacteriostatic or bactericidal activities. One third of the tested QSIs, including the plant compound hordenine and the human sexual hormone estrone, decreased the frequency of the QS-regulated horizontal transfer of the tumor-inducing (Ti) plasmid in A. tumefaciens. Hordenine, estrone as well as its structural relatives estriol and estradiol, also decreased AHL accumulation and the expression of six QS-regulated genes (lasI, lasR, lasB, rhlI, rhlR, and rhlA) in cultures of the opportunist pathogen P. aeruginosa. Moreover, the ectopic expression of the AHL-receptors RhlR and LasR of P. aeruginosa in E. coli showed that their gene-regulatory activity was affected by the QSIs. Finally, modeling of the structural interactions between the human hormones and AHL-receptors LasR of P. aeruginosa and TraR of A. tumefaciens confirmed the competitive binding capability of the human sexual hormones. This work indicates potential interferences between bacterial and eukaryotic hormonal communications.

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Section: Discussionmentioning

confidence: 99%

At a Supra-Physiological Concentration, Human Sexual Hormones Act as Quorum-Sensing Inhibitors

et al. 2013

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“…30,31 Proteinaceous estrogen-binding receptors have been reported in P. aeruginosa and Escherichia coli. [32][33][34] P. aeruginosa can actively metabolize steroid hormones 35 and use estradiol as a carbon source. 36 In our study, estriol, although less potently estrogenic than estradiol, was a stronger inducer of alginate.…”

Section: Discussionmentioning

confidence: 99%

Effect of Estrogen on Pseudomonas Mucoidy and Exacerbations in Cystic Fibrosis

et al. 2012

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“…Estradiol and proteins that bind it can be found in a wide range of species, including unicellular organisms [60,171], and application of estradiol can alter their behavior [99]. The earliest steroid receptors, which evolved more than 600 million years ago prior to the split between deuterostomes and protostomes, functioned as estrogen receptors [203].…”

Section: Evolution Of Estrogen-dependent Sensory Processesmentioning

confidence: 99%

Estradiol-dependent modulation of auditory processing and selectivity in songbirds

Maney

Pinaud

2011

Frontiers in Neuroendocrinology

100

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The steroid hormone estradiol plays an important role in reproductive development and behavior and modulates a wide array of physiological and cognitive processes. Recently, reports from several research groups have converged to show that estradiol also powerfully modulates sensory processing, specifically, the physiology of central auditory circuits in songbirds. These investigators have discovered that (1) behaviorally-relevant auditory experience rapidly increases estradiol levels in the auditory forebrain; (2) estradiol instantaneously enhances the responsiveness and coding efficiency of auditory neurons; (3) these changes are mediated by a non-genomic effect of brain-generated estradiol on the strength of inhibitory neurotransmission; and (4) estradiol regulates biochemical cascades that induce the expression of genes involved in synaptic plasticity. Together, these findings have established estradiol as a central regulator of auditory function and intensified the need to consider brain-based mechanisms, in addition to peripheral organ dysfunction, in hearing pathologies associated with estrogen deficiency.

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Identification of an estrogen-binding protein in Pseudomonas aeruginosa

Cited by 25 publications

References 23 publications

At a Supra-Physiological Concentration, Human Sexual Hormones Act as Quorum-Sensing Inhibitors

At a Supra-Physiological Concentration, Human Sexual Hormones Act as Quorum-Sensing Inhibitors

Effect of Estrogen on Pseudomonas Mucoidy and Exacerbations in Cystic Fibrosis

Estradiol-dependent modulation of auditory processing and selectivity in songbirds

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