Identification of an organelle receptor for myosin-Va

Wu, Xufeng; Rao, Kang; Zhang, Hong; Wang, Fei; Sellers, James R.; Matesic, Lydia E.; Copeland, Neal G.; Jenkins, Nancy A.; Hammer, John A.

doi:10.1038/ncb760

Cited by 436 publications

(466 citation statements)

References 35 publications

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“…In melanocytes lacking Rab27a or expressing dominant negative Rab27a mutants, melanosomes are not correctly positioned in dendritic tips and remain clustered in the cell center Wu et al, 2002). The morphology of INS-1E cells is often characterized by the presence of dendritic-like protrusions in which insulin-containing secretory granules accumulate.…”

Section: Resultsmentioning

confidence: 99%

“…In melanocytes loss of function mutations in the Rab27a gene lead to a defect in melanosome distribution Wu et al, 2002) and in cytotoxic T lymphocytes to impairment in granule exocytosis (Stinchcombe et al, 2001;Haddad et al, 2001). The mechanism of action of Rab27a in melanocytes has been intensively investigated and is now relatively well understood.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of action of Rab27a in melanocytes has been intensively investigated and is now relatively well understood. The activation of Rab27a determines the binding of Slac2a/melanophilin to melanosomes Wu et al, 2002). This causes the recruitment of the actin-based motor protein Myosin-Va to the organelle surface and allows peripheral retention of melanosomes within the cortical actin network (Wu et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Involvement of the Rab27 Binding Protein Slac2c/MyRIP in Insulin Exocytosis

Waselle

Coppola

Fukuda

et al. 2003

MBoC

153

159

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Involvement of the Rab27 Binding Protein Slac2c/MyRIP in Insulin Exocytosis

Waselle

Coppola

Fukuda

et al. 2003

MBoC

153

159

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“…During aggregation, the release of myosin V helps the dynein-mediated movement to 'win' over the kinesin-2-mediated movement 145 . In mammalian melanocytes, myosin V is recruited to melanosomes by RAB27a through melanophilin, a RAB-binding protein 146 , in preparation for melanosome transfer to keratinocytes. In this cell type, myosin V and melanophilin track the microtubule plus end through an interaction with microtubuleplus-end-binding protein-1 (EB1) 147 .…”

Section: Box 3 | Coordinating Several Motors: the Melanosome Paradigmmentioning

confidence: 99%

Powering membrane traffic in endocytosis and recycling

Soldati

Schliwa

2006

Nat Rev Mol Cell Biol

314

279

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| Early in evolution, the diversification of membrane-bound compartments that characterize eukaryotic cells was accompanied by the elaboration of molecular machineries that mediate intercompartmental communication and deliver materials to specific destinations. Molecular motors that move on tracks of actin filaments or microtubules mediate the movement of organelles and transport between compartments. The subjects of this review are the motors that power the transport steps along the endocytic and recycling pathways, their modes of attachment to cargo and their regulation.The emergence of eukaryotic cells was accompanied by the development of endomembrane systems that compartmentalize biochemical pathways and biosynthetic processes. An evolutionary trend towards increasing complexity and subcellular specialization necessitated the development of machineries for intercompartmental communication. Molecular assemblies evolved to confer specificity to the interactions of donor and acceptor compartments (budding, sorting, tethering and fusion). Cytoskeletal polymers such as actin filaments and microtubules, which help segregate genetic material and determine cell shape and subcellular architecture, were co-opted as tracks for transport. In animal cells, microtubules support long-range transport, whereas the more flexible actin filaments serve short-range movements both near the cell periphery and in the cell interior. Also, actin filaments can be woven into dense networks of short fibres through the action of crosslinkers and branchpromoting complexes. On the other hand, in many plant cells, bundled actin filaments can form extended tracks for long-distance transport. Owing to the gel-like nature of the cytoplasm, the Brownian movement of organelles is severely restricted and cargoes have to be transported to their destinations at the expense of energy. Furthermore, seemingly random, but motor-dependent, movement is proposed to increase the probability of vesicle collisions and therefore promote fusion events. Movement is powered by three ATP-dependent motors: myosin, kinesin and dynein. Over the course of eukaryotic evolution, these motors have diversified into a large number of families with specific tasks (FIG. 1).Here, we review the involvement of molecular motors in the movement of endosomes and in vesicular trafficking along the endocytic and recycling pathways. These pathways are summarized in FIG. 2. We do not, however,

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“…In melanocytes, Rab27a associates with the melanosome membrane and is most concentrated in melanosome-rich dendritic tips (Wu et al, 2001). Melanophilin (also known as Slac2) is a synaptotagmin-like protein that binds both the Rab27a and Myosin Va proteins (Strom et al, 2002;Wu et al, 2002). The N-terminus of melanophilin interacts with Rab27a and the central region with the region encoded by exon F of the globular tail of MyosinVa.…”

Section: Melanosome Transportmentioning

confidence: 99%

Mouse coat color mutations: From fancy mice to functional genomics

Steingrı́msson

Copeland

Jenkins

2006

Developmental Dynamics

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Mouse coat color mutations have a long history in biomedical research. The viable and visible phenotype of most coat color mutations has made the pigment cell, the melanocyte, an ideal system for genetic, molecular, and cellular analysis. Molecular cloning and analysis of many of the different coat color mutations have revealed the roles of a diverse range of genes, and today we know more about the pathways and proteins that regulate the development and function of pigment cells than we know about most other cell types in mammalian organisms. Coat color mutations have also provided novel insights into stem cell biology and human diseases, including melanoma. In the future, it will be important to build on this history and knowledge by taking advantage of the extensive repertoire of recently developed genome-wide methodologies, available genomic information, and the powerful methods that have been developed for modifying the mouse genome to systematically dissect the development and function of this important cell type. The usefulness of coat color mutations has just begun to emerge. Developmental Dynamics 235: 2401-2411, 2006.

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Identification of an organelle receptor for myosin-Va

Cited by 436 publications

References 35 publications

Involvement of the Rab27 Binding Protein Slac2c/MyRIP in Insulin Exocytosis

Involvement of the Rab27 Binding Protein Slac2c/MyRIP in Insulin Exocytosis

Powering membrane traffic in endocytosis and recycling

Mouse coat color mutations: From fancy mice to functional genomics

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