Identification of an RNA fraction from Enterococcus faecalis FK-23 cells as the antihypertensive compound

Shimada, Takashi; Kadowaki, Yasuhiro; Ohmiya, Kunio; Yamamoto, Tetsuro

doi:10.1016/0922-338x(96)85030-6

Cited by 8 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recent clinical study, we have observed that the number of peripheral blood eosinophils reduced significantly after LFK treatment in patients with perennial allergic rhinitis (unpublished data). In addition, FK23, a heat‐killed preparation made from the same strain of E. faecalis , has been demonstrated to be capable of some activities important for maintenance of human health, such as anti‐tumour [4, 5], immunomodulated [6, 7] and anti‐hypertensive effects [8].…”

Section: Introductionmentioning

confidence: 99%

Effect of lysed Enterococcus faecalis FK‐23 (LFK) on allergen‐induced peritoneal accumulation of eosinophils in mice

Shimada¹,

Cheng

Ide³

et al. 2003

Clin Experimental Allergy

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of lysed Enterococcus faecalis FK‐23 (LFK) on allergen‐induced peritoneal accumulation of eosinophils in mice

Shimada¹,

Cheng

Ide³

et al. 2003

Clin Experimental Allergy

View full text Add to dashboard Cite

show abstract

“…It has been reported that its immunostimulatory action could be enhanced by heat sterilization. 28,29) A variety of beneficial effects, including regulation of intestine 30,31) and hypotensive action 32) have been observed in animal and clinical studies using heat-killed E. faecalis. To investigate these beneficial effects of E. faecalis, we evaluated the IL-12-inducing activity of 10 strains of E. faecalis using a mouse macrophage-like cell line, J774.1.…”

mentioning

confidence: 99%

RNA and a cell wall component of Enterococcus faecalis IC-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line J774.1

Nakase¹,

Ukawa²,

Takemoto³

et al. 2017

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Enterococcus faecalis is a resident lactic acid bacterium in the human intestine. Its immunostimulatory action was reported to be enhanced by heat sterilization. To investigate its beneficial actions, we evaluated the ability of 10 E. faecalis strains to induce interleukin-12 (IL-12) production in a mouse macrophage cell line, J774.1 and found that the strain, E. faecalis IC-1, had a potent IL-12-inducing ability. Furthermore, we investigated the underlying mechanism by treating IC-1 cells with RNase or lysozyme. Its activity almost disappeared and an antagonist of Toll-like receptor (TLR) 7 inhibited this activity. Moreover, lysozyme-treated IC-1 bacteria were not phagocytized by J774.1 cells, and did not induce IL-12 production. Based on our results, we propose that macrophages recognize the cell wall components of IC-1, leading to phagocytosis. The IC-1 RNA is then recognized by TLR7, which induces the production of IL-12.

show abstract

“…We have focused on Enterococcus faecalis strain FK-23. Heat-treated FK-23 possessed functionality expressed as anti-tumour and anti-microbial activities ( 12 ) , immunomodulatory effects ( 13 , 14 ) and anti-hypertensive activity ( 15 ) . In addition, FK-23 processed by bacteriolytic enzyme- and heat-treatment exhibited anti-allergic activities ( 16 – 18 ) .…”

mentioning

confidence: 99%

Effects ofEnterococcussp. isolated from deep seawater on inhibition of allergic responses in mice

et al. 2008

Self Cite

View full text Add to dashboard Cite

The objective of the present study was to assess the effects of Enterococcus sp. strain TN-3 isolated from deep seawater on inhibition of eosinophil accumulation, IgE production and active cutaneous anaphylaxis (ACA). We investigated the effects of viable and non-viable TN-3 on allergeninduced peritoneal eosinophil accumulation in mice. Viable (5·4 £ 10 10 colony-forming units per 60 mg) or non-viable TN-3 (60 mg) was orally administered to BALB/c mice that had been sensitised with the cedar pollen (Cryptomeria japonica) allergen. Oral administration of non-viable TN-3 was effective in suppressing eosinophil accumulation while viable TN-3 was ineffective. We also examined the dose -response relationship for non-viable TN-3 in regard to eosinophil accumulation, IgE production and ACA in allergen-primed mice. Non-viable TN-3 was orally administered at doses of 15 mg (low dose), 30 mg (medium dose) and 60 mg (high dose) to BALB/c mice that had been sensitised with cedar pollen allergen. The anti-allergic effects expressed as inhibition of eosinophil accumulation, IgE production and ACA were found at the low and high doses, but not at the medium dose. These results suggest that non-viable TN-3 exhibited anti-allergic effects at doses of 15 and 60 mg.Allergy: Viability: Dose -response relationships: Enterococcus sp.

show abstract

Identification of an RNA fraction from Enterococcus faecalis FK-23 cells as the antihypertensive compound

Cited by 8 publications

References 14 publications

Effect of lysed Enterococcus faecalis FK‐23 (LFK) on allergen‐induced peritoneal accumulation of eosinophils in mice

Effect of lysed Enterococcus faecalis FK‐23 (LFK) on allergen‐induced peritoneal accumulation of eosinophils in mice

RNA and a cell wall component of Enterococcus faecalis IC-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line J774.1

Effects ofEnterococcussp. isolated from deep seawater on inhibition of allergic responses in mice

Contact Info

Product

Resources

About