Identification of androgen response‐related lncRNAs in prostate cancer

Abstract

Background: Long noncoding RNAs (lncRNAs) are RNA molecules with over 200 nucleotides that do not code for proteins, but are known to be widely expressed and have key roles in gene regulation and cellular functions. They are also found to be involved in the onset and development of various cancers, including prostate cancer (PCa). Since PCa are commonly driven by androgen regulated signaling, mainly stimulated pathways, identification and determining the influence of lncRNAs in androgen response is useful and necessary. LncRNAs regulated by the androgen receptor (AR) can serve as potential biomarkers for PCa. In the present study, gene expression data analysis were performed to distinguish lncRNAs related to the androgen response pathway. Methods and Results:We used publicly available RNA-sequencing and ChIP-seq data to identify lncRNAs that are associated with the androgen response pathway. Using Universal Correlation Coefficient (UCC) and Pearson Correlation Coefficient(PCC) analyses, we found 15 lncRNAs that have (a) highly correlated expression with androgen response genes in PCa and are (b) differentially expressed in the setting of treatment with an androgen agonist as well as antagonist compared to controls.Using publicly available ChIP-seq data, we investigated the role of androgen/AR axis in regulating expression of these lncRNAs. We observed AR binding in the promoter regions of 5 lncRNAs (MIR99AHG, DUBR, DRAIC, PVT1, and COLCA1), showing the direct influence of AR on their expression and highlighting their association with the androgen response pathway. Conclusion:By utilizing publicly available multiomics data and by employing in silico methods, we identified five candidate lncRNAs that are involved in the androgen response pathway. These lncRNAs should be investigated as potential biomarkers for PCa.

Comprehensive data mining reveals RTK/RAS signaling pathway as a promoter of prostate cancer lineage plasticity through transcription factors and CNV

“…S5 ). Among these genes, SOX5 29 , PRKCB 40 , VCL 41 , ARSB 42 , TF (Transferrin) 43 , 44 , APC 45 , PTEN 46 , TP53 47 , BRCA1 48 , DCP2 49 , DCUN1D2 50 , MKLN1 51 , LPAR1 52 , EFNA5 and SORBS1 53 , 54 have been reported play important roles in prostate cancer. COL14A1 55 , ANGPT1 56 , SERPINF1 57 , GJA1 58 , SMC2 59 , PCID2 60 , FBXL8 61 , and FBXL17 62 have also been reported in other cancers.…”

Long non‑coding RNA DANCR aggravates breast cancer through the miR‑34c/E2F1 feedback loop

“…According to previous studies, long non-coding RNAs (lncRNAs) serve an important role in human cancer ( 5 , 6 ). Numerous types of human cancer cells exhibit dysregulated expression of these ncRNA molecules, which are ~200 nucleotides in length and are transcribed from the corresponding gene locus ( 7 , 8 ). LncRNAs exert biological functions through the epigenetic modulation of transcriptional and post-transcriptional regulation in physiological and pathological activities.…”