Identification of Androstenedione in a River Containing Paper Mill Effluent

Jenkins, Ronald L.; Angus, Robert A.; McNatt, Heather; Howell, W. Mike; Kemppainen, Jon; Kirk, Marion; Wilson, Elizabeth M.

doi:10.1897/1551-5028(2001)020<1325:ioaiar>2.0.co;2

Cited by 57 publications

(90 citation statements)

References 32 publications

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“…It has been hypothesized that bacterial biotransformation of plant sterols in the effluent generates the androgens responsible for the observed masculinization of female mosquitofish exposed to paper mill effluent (49). Recently, it was reported that androstenedione was present after a chemical characterization of the effluent from a paper mill plant in Florida (50). Using a number of in vitro receptor binding and gene expression assays, our laboratory in collaboration with others has not found sufficient androstenedione concentration in Fenholloway River water samples to support the reported significant androgenic actions of this effluent, however (15).…”

mentioning

confidence: 91%

Aromatase activity in the ovary and brain of the eastern mosquitofish (Gambusia holbrooki) exposed to paper mill effluent.

Orlando

Davis

Guillette

2002

Environ Health Perspect

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Endocrine DisruptorsStudies have shown that female mosquitofish living downstream of a paper mill located on the Fenholloway River, Florida, have masculinized secondary sex characteristics, including altered anal fin development and reproductive behavior. Masculinization can be caused by exposure to androgens in the water or from an alteration in aromatase activity in the fish. We hypothesized that aromatase activity would be inhibited by a component(s) of the paper mill effluent. Aromatase inhibition could masculinize the hormonal profile and, subsequently, secondary sex characteristics of the exposed females. Therefore, we predicted that ovarian and brain aromatase activity would be lower in the female mosquitofish from the Fenholloway River compared with the reference site, the Econfina River. Adult females were collected and standard length, body mass, anal fin length, and segment number were measured. Ovarian and brain aromatase activity were determined using a tritiated water assay.

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mentioning

confidence: 91%

Aromatase activity in the ovary and brain of the eastern mosquitofish (Gambusia holbrooki) exposed to paper mill effluent.

Orlando

Davis

Guillette

2002

Environ Health Perspect

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“…Special attention has been directed toward plant sterols metabolites as androstenedione for being an active hormonally compound capable of inducing androgenic effects in organisms (Malaviya and Gomes 2008;Lambert and Edwards 2017). This compound has been found in kraft mill effluents, even after being treated by activated sludge (Chamorro et al 2016a) at a concentration of 2.4 nM sediment near to discharge and 0.14 nM in the water column (Jenkins et al 2001(Jenkins et al , 2003; Van den Heuvel et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Sensitivity Study Comparing Daphnia obtusa (Kurz 1874) and Daphnia magna (Straus 1820) Exposure to Treated Kraft Mill Effluents, Diethylstilbestrol, and Androstenedione

et al. 2017

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Kraft mill effluents treated by activated sludge show a reduction in acute toxicity. However, their discharges produce hormonal effects in an aquatic ecosystem, due to the sterols metabolites as androstenedione contained in the effluents. Daphnids bioassays are a powerful approach for determining toxicity. However, there are relative sensitivities depending on the species. The main objective of this study is to determine the sensitivity of D. magna and D. obtusa when exposed to kraft mill effluents, diethylstilboestrol (DES), and androstenedione (AED). The sensitivities were tested using acute bioassay exposed to kraft pulp mill. Moreover, the allometric growth rate of both daphnids affected by DES and AED regarding time were also evaluated for a period of nine days. Variation in the ratio between body length and body width -at the abdominal cavity -over time (k index) was evaluated. Results indicated that AED and DES compounds affected the allometric growth rate of daphnids. Specifically D. magna exhibited more sensitivity when it was exposed to kraft pulp mill.

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“…Moreover, phytosterols in pulp and paper mill effluents were transformed to androgens by microorganisms in river sediments, consequently influencing fish development (Jenkins et al, 2001(Jenkins et al, , 2003. Steroid hormones have been frequently detected in various environmental matrices, including soil, groundwater, surface water and sediments (Ying et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

et al. 2016

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Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotopelabeled substrates are not easily available.

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Identification of Androstenedione in a River Containing Paper Mill Effluent

Cited by 57 publications

References 32 publications

Aromatase activity in the ovary and brain of the eastern mosquitofish (Gambusia holbrooki) exposed to paper mill effluent.

Aromatase activity in the ovary and brain of the eastern mosquitofish (Gambusia holbrooki) exposed to paper mill effluent.

Sensitivity Study Comparing Daphnia obtusa (Kurz 1874) and Daphnia magna (Straus 1820) Exposure to Treated Kraft Mill Effluents, Diethylstilbestrol, and Androstenedione

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

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