Identification of Angiotensin I-converting enzyme Inhibitory Activities from traditional Mongolian fermented milk products

Abstract

Several angiotensen-converting enzyme (ACE) inhibitory peptites have been detected in milk products. There are many traditional milk products in Mongolia. For this study, some Mongolian milk products were collected, and the ACE inhibitory activities of these samples were tested; an active fraction was found in aaruul made from mare's milk. After purification by dialysis and HPLC, the active fractions were isolated. The molecular weight of the active component was 362.05 M, as determined by mass spectrometry. An authentic standard was used to determine the IC 50 value of the inhibitory activity. From 5 ' -GMP is not much higher than that of the active peptide in sour milk and some flavonoids. However, this is the first report that shows that 5 ' -GMP inhibits ACE activity. These results will provide useful information for the development of hypertension therapy agents.

Inhibition Activity of Angiotensin Converting Enzyme (ACE) and Determination of Total Phenolic and Flavonoid Compound from Bitter Melon Leaves (Momordica charantia L.)

“…Study procedures were based on ACE inhibition test using ACE kit-WST which had been done in the previous study. 5 The sample was tested to obtain ACE inhibition percentage from 80% ethanolic extract of bitter melon leaves and IC 50 value determination. Results of the study showed that ACE inhibition percentage of the study sample was 93.66% in 100 ppm.…”

Angiotensin Converting Enzyme Inhibitory Activity of Melinjo (Gnetum Gnemon L.) Seed Extracts and Molecular Docking of Its Stilbene Constituents

“…The study procedure was based on the ACE inhibitory test using ACE kit-WST which had been done in the previous study [24]. In this study, the ACE inhibitory activity was stated as inhibitory percentage values.…”