Identification of anthocyanins in wines by liquid chromatography, liquid chromatography-mass spectrometry and nuclear magnetic resonance

Košir, Iztok Jože; Lapornik, Brigita; Andrenšek, Samo; Wondra, Alenka Golc; Vrhovšek, Urška; Kidrič, J.

doi:10.1016/j.aca.2003.12.013

Cited by 51 publications

(6 citation statements)

References 21 publications

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“…Forveille et al used HMBC-GAS, HMQC, and HMQC-HOHAHA 2D maps to differentiate grapevine cultivars in an ANOVA analysis of the vine polyphenols , A series of recent publications by Kosir and Kidric et al deals with the chemometric analysis of wines. − While large (solvent) signal suppression is reported to be an important general prerequisite, a signal intensity-based qHNMR analysis could even be achieved for minor constituents such as anthocyanin glycosides and amino acids in a semiquantitative fashion after chromatographic enrichment. , More recently, the qHNMR determination of aging indicators in balsamic vinegars has been demonstrated to supplement their quality assessment . In this context it is noteworthy that the qHNMR observation of deuterium in site-specific natural isotope fractionation (SNIF-NMR) became the first officially adopted stable isotope method in wine analysis in the European Union in 1990, , allowing the discrimination between natural and enriched wines and differentiation of geographical origins .…”

Section: Applications Of Qhnmrmentioning

confidence: 99%

Quantitative¹H NMR: Development and Potential of a Method for Natural Products Analysis

2004

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Based on a brief revision of what constitutes state-of-the-art "quantitative experimental conditions" for (1)H quantitative NMR (qHNMR), this comprehensive review contains almost 200 references and covers the literature since 1982 with emphasis on natural products. It provides an overview of the background and applications of qHNMR in natural products research, new methods such as decoupling and hyphenation, and analytical potential and limitations, and compiles information on reference materials used for and studied by qHNMR. The dual status of natural products, being single chemical entities and valuable biologically active agents that need to be purified from complex matrixes, results in an increased analytical demand when testing their deviation from the singleton composition ideal. The outcome and versatility of reported applications lead to the conclusion that qHNMR is currently the principal analytical method to meet this demand. Considering both 1D and 2D (1)H NMR experiments, qHNMR has proved to be highly suitable for the simultaneous selective recognition and quantitative determination of metabolites in complex biological matrixes. This is manifested by the prior publication of over 80 reports on applications involving the quantitation of single natural products in plant extracts, dietary materials, and materials representing different metabolic stages of (micro)organisms. In summary, qHNMR has great potential as an analytical tool in both the discovery of new bioactive natural products and the field of metabolome analysis.

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Section: Applications Of Qhnmrmentioning

confidence: 99%

Quantitative¹H NMR: Development and Potential of a Method for Natural Products Analysis

2004

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“…Fourier transform near-infrared and 1 H NMR spectroscopy techniques combined with classification methods were used for the authentication of anthocyanin content of red wines, in an effort to detect possible adulteration with black rice anthocyanins [37]. By the combination of NMR and LC-MS, the identification of anthocyanins in Pinot Noir, Cabernet Sauvignon, and Merlot red wines produced in Slovenia was obtained [38]. High-performance liquid chromatography coupled with mass spectrometry (LC-MS) and NMR spectrometry (LC-NMR) were used to analyze the composition of anthocyanins of grape berry skins belonging to four different grape varieties [39].…”

Section: Phenolicsmentioning

confidence: 99%

NMR-based metabolomics in wine quality control and authentication

Amargianitaki

Spyros

2017

Chem. Biol. Technol. Agric.

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A comprehensive summary of research work related to applications of NMR spectroscopy in combination with multivariate statistical analysis techniques for the analysis, quality control, and authentication of wine is presented. NMR spectroscopy is used to obtain the non-volatile metabolic profile and/or phenolic profile of wines, with the help of 2D NMR spectroscopy. Metabolomics is then used as an analytical tool to investigate the variability of the metabolic profile of wines due to a series of different factors involved during wine production, including terroir, pedoclimatic conditions, vintage, vineyard practices, wine-making, barrel maturation, and aging. The discriminating power of the NMR-obtained wine metabolome is utilized as a wholistic analytical approach in efforts to authenticate wine with respect to important economic attributes, such as cultivar, vintage, and geographical origin.

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“…Their elution order and absorbance spectra confirmed the identity of the supposed anthocyanins. As known, the polarity of the aglycon (anthocyanidin) is the most important factor affecting the HPLC retention time, and the typical order of elution of the different anthocyanins with similar glycosylation patterns is as follows: first delphinidin derivatives, followed by cyanidin, petunidin, pelargonidin, peonidin, and finally malvidin derivatives ( , ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Anthocyanins in Rhamnus alaternus L. Berries

Longo

Vasapollo

Rescio

2005

J. Agric. Food Chem.

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Anthocyanin composition in berries of Rhamnus alaternus L., a perennial wild shrub typical of the Mediterranean area, was determined for the first time. The pigments were extracted from the berries with 0.1% HCl in methanol and purified using a C-18 solid-phase cartridge. High-performance liquid chromatography diode array detection-mass spectrometry analysis showed that delphinidin 3-O-rutinoside represented about 62.4% of the total pigments. Other anthocyanins were 3-O-rutinoside derivatives of cyanidin (8.4%), petunidin (15.8%), pelargonidin (4.7%), and peonidin and malvidin (8.7%). The concomitant presence of the six most common anthocyanidins suggested that R. alaternus berries, besides being a good pigment source, could also be a useful tool for anthocyanin identification.

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Identification of anthocyanins in wines by liquid chromatography, liquid chromatography-mass spectrometry and nuclear magnetic resonance

Cited by 51 publications

References 21 publications

Quantitative¹H NMR: Development and Potential of a Method for Natural Products Analysis

Quantitative¹H NMR: Development and Potential of a Method for Natural Products Analysis

NMR-based metabolomics in wine quality control and authentication

Identification of Anthocyanins in Rhamnus alaternus L. Berries

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Identification of anthocyanins in wines by liquid chromatography, liquid chromatography-mass spectrometry and nuclear magnetic resonance

Cited by 51 publications

References 21 publications

Quantitative1H NMR: Development and Potential of a Method for Natural Products Analysis

Quantitative1H NMR: Development and Potential of a Method for Natural Products Analysis

NMR-based metabolomics in wine quality control and authentication

Identification of Anthocyanins in Rhamnus alaternus L. Berries

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Product

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Quantitative¹H NMR: Development and Potential of a Method for Natural Products Analysis

Quantitative¹H NMR: Development and Potential of a Method for Natural Products Analysis