Identification of anti‐<scp>TMV</scp> active flavonoid glycosides and their mode of action on virus particles from <scp><i>Clematis lasiandra</i> Maxim</scp>

Li, Yantao; Ye, Shengwei; Hu, Ziyi; Nan, Hao; Bo, Xin; Liang, Huaguang; Tian, Xiangrong

doi:10.1002/ps.6569

Cited by 25 publications

(15 citation statements)

References 53 publications

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“…The levels of tocopherol, flavonoids, phenylpropane and ascorbic acid precursors in the seeds of heat-resistant soybean genotypes were higher after heat stress [ 21 ]. The active flavonoid glycosides in Clematis lasiandra provide effective anti-tobacco mosaic virus (TMV) activity through the TMV-CP target [ 22 ]. Heat stress leads to upregulation of the oxylipin biosynthetic process and proline biosynthetic process in Agrostis stolonifera [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

Qian

et al. 2022

BMC Plant Biol

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Background Clematis species are attractive ornamental plants with a variety of flower colors and patterns. Heat stress is one of the main factors restricting the growth, development, and ornamental value of Clematis. Clematis lanuginosa and Clematis crassifolia are large-flowered and evergreen Clematis species, respectively, that show different tolerance to heat stress. We compared and analyzed the transcriptome of C. lanuginose and C. crassifolia under heat stress to determine the regulatory mechanism(s) of resistance. Results A total of 1720 and 6178 differentially expressed genes were identified from C. lanuginose and C. crassifolia, respectively. The photosynthesis and oxidation–reduction processes of C. crassifolia were more sensitive than C. lanuginose under heat stress. Glycine/serine/threonine metabolism, glyoxylic metabolism, and thiamine metabolism were important pathways in response to heat stress in C. lanuginose, and flavonoid biosynthesis, phenylalanine metabolism, and arginine/proline metabolism were the key pathways in C. crassifolia. Six sHSPs (c176964_g1, c200771_g1, c204924_g1, c199407_g2, c201522_g2, c192936_g1), POD1 (c200317_g1), POD3 (c210145_g2), DREB2 (c182557_g1), and HSFA2 (c206233_g2) may be key genes in the response to heat stress in C. lanuginose and C. crassifolia. Conclusions We compared important metabolic pathways and differentially expressed genes in response to heat stress between C. lanuginose and C. crassifolia. The results increase our understanding of the response mechanism and candidate genes of Clematis under heat stress. These data may contribute to the development of new Clematis varieties with greater heat tolerance.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

Qian

et al. 2022

BMC Plant Biol

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“…16,17 In addition, flavonoids also have been identified as promising anti-TMV agents. Some flavonoids with notable anti-TMV activity, such as flavonoid glycosides from Lasiandra maxim, 18 flavones from leaves of Nicotiana tabacum, 19 chalcones from Desmodium podocarpum, 20 isoflavones from the Pueraria montana, 21 and the like, have also been reported in previous literature.…”

Section: Introductionmentioning

confidence: 59%

“…The docking results reveal that the flavonoids nucleus maybe fundamental for anti-TMV activity, and this result is consistent with that of pervious reported literature. 18,19,28 In addition, the (3-methyl-1Hpyrrol-2-yl)methyl or (4-methylfuran-2-yl)methyl moiety substituted on flavones may increase the inhibitory activities, and the (3-methyl-1H-pyrrol-2-yl)methyl moiety has better effect than that of (4-methylfuran-2-yl)methyl moiety. This structure-activity relationship is for the first time proposed in our study, and is helpful to find new anti-TMV activity inhibitors.…”

Section: 7mentioning

confidence: 99%

C‐Alkylated flavonoids from the whole plants of Desmodium caudatum and their anti‐TMV activity

Xiong

et al. 2023

Pest Management Science

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BackgroundNatural products are important sources of biopesticides to control plant virus, and flavonoids are identified as promising anti‐tobacco mosaic virus (TMV) agents. Since Desmodium caudatum is a rich source of flavonoids, this study focuses on the discovery of the new anti‐TMV active flavonoids from D. caudatum and their possible mode of action.ResultsThree new (compounds 1–3) and nine known (compounds 4–12) C‐alkylated flavonoids were isolated from D. caudatum. To the best of our knowledge, the framework of 1–3 was reported in natural products for the first time. In addition, 1–3, 5, and 6 showed notable anti‐TMV activity with inhibition rates in the range of 35.8–64.3% at a concentration of 50 μg/mL, and these rates are higher than that of positive control (with inhibition rates of 34.6% ± 2.8). In addition, the structure–activity relationship study revealed that the (pyrrol‐2‐yl)methyl moiety on flavone can significantly increases the activity. This result is helpful to find new anti‐TMV inhibitors.ConclusionC‐Alkylated flavonoids showed potent activities against TMV with multiple modes of actions. The increase of defense‐related enzyme activities, up‐regulate the expression of defense related genes, down‐regulate the expression of Hsp70 protein by inhibiting the related Hsp genes that are involved in tobacco resistance to TMV. By the actions mentioned earlier, the infection of TMV was influenced, thereby achieving the effects of control of TMV. The successful isolation of the earlier‐mentioned flavonoids provide the new source of biopesticides to TMV proliferation, and also contribute to the utilization of D. caudatum. © 2023 Society of Chemical Industry.

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“…Refractory plant diseases critically reduce the yield and quality of crops, fruits, vegetables, and ornamental plants, resulting in severe pressures in global agriculture. [1][2][3][4] For example, plant viruses widely parasitize plant hosts, including tobacco, tomato, pepper and potato, and spread around the world, resulting in mosaic or deformed leaf symptoms and decreased crop production. Thus, plant viruses characterized by their extreme contagiousness and destructiveness are referred to as "plant cancer."…”

Section: Introductionmentioning

confidence: 99%

Novel cinnamic acid derivatives as a versatile tool for developing agrochemicals for controlling plant virus and bacterial diseases by enhancing plant defense responses

Yang

Liu

et al. 2023

Pest Management Science

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BACKGROUND Plant pathogens have led to large yield and quality losses in crops worldwide. The discovery and study of novel agrochemical alternatives based on the chemical modification of bioactive natural products is a highly efficient approach. Here, two series of novel cinnamic acid derivatives incorporating diverse building blocks with alternative linking patterns were designed and synthesized to identify their antiviral capacity and antibacterial activity. RESULTS The bioassay results demonstrated that most cinnamic acid derivatives had excellent antiviral competence toward tobacco mosaic virus (TMV) in vivo, especially compound A5 (median effective concentration [EC50] = 287.7 μg mL−1), which had a notable protective effect against TMV when compared with the commercial virucide ribavirin (EC50 = 622.0 μg mL−1). In addition, compound A17 had a protective efficiency of 84.3% at 200 μg mL−1 against Xac in plants. Given these outstanding results, the engineered title compounds could be regarded as promising leads for controlling plant virus and bacterial diseases. Preliminary mechanistic studies suggest that compound A5 could enhance the host's defense responses by increasing the activity of defense enzymes and upregulating defense genes, thereby suppressing phytopathogen invasion. CONCLUSION This research lays a foundation for the practical application of cinnamic acid derivatives containing diverse building blocks with alternative linking patterns in pesticide exploration. © 2023 Society of Chemical Industry.

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Identification of anti‐TMV active flavonoid glycosides and their mode of action on virus particles from Clematis lasiandra Maxim

Cited by 25 publications

References 53 publications

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

Comparative transcriptome analysis of heat stress responses of Clematis lanuginosa and Clematis crassifolia

C‐Alkylated flavonoids from the whole plants of Desmodium caudatum and their anti‐TMV activity

Novel cinnamic acid derivatives as a versatile tool for developing agrochemicals for controlling plant virus and bacterial diseases by enhancing plant defense responses

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