Identification of Antifungal Compounds From Leaf Extract of Eucalyptus Citriodora Against Ascochyta Rabiei

Abstract

Ascochyta rabiei (Pass.) Lab. is a highly destructive pathogen of chickpea. In thisstudy, leaf extract of Eucalyptus citriodora was assessed against this pathogen. Bioassays withmethanolic extract (0, 05, 1.0, 1.5, …, 4.0%) extract revealed the remarkable antifungal potential ofleaf extract where 69–94% reduction in biomass of A. rabiei was recorded. Chloroform fraction of thisextract was separated by partitioning the extract in a separating funnel. Using a solvent system ofchloroform: n-hexane (20:80), three compounds were detected on TLC plate which were separatedthrough preparative TLC and purified on HPLC. GC-MS of the purified compounds lead to theidentification of 3-cyclohexene- 1-ol, 4-methyl-1-(1-methylethyl)- (1), 1-cyclohexene- 1carboxaldehyde,4-(1-methylethyl)- (2) and eucalyptol (3) that might caused antifungal activity of the extract.