Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera

Kumar, Susheel; Kaushik, Nutan; Proksch, Peter

doi:10.1186/2193-1801-2-37

Cited by 34 publications

(17 citation statements)

References 16 publications

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“…Endophytic fungus was isolated from the medicinal plant of Laurus sp. according to with the surface sterilization method [16]. The surface of stem and leaf was sterilized with 70% ethanol for 2 min followed by 1% sodium hypochlorite for 3 min.…”

Section: Isolation Of Endophytic Fungus Trichoderma Sp Efi 671mentioning

confidence: 99%

“…EFI 671 was cultivated on 200 g of sterilized rice medium in a 1000 mL conical flask for metabolite extraction as per the method of [18]. After 15 days of growth at 25 • C under dark conditions, metabolites were extracted using 250 mL of ethyl acetate (EtOAc) solvent according [16]. The organic solvent was evaporated to dryness in a rotary evaporator (Heidolph, Germany), yielding 500 mg of ethyl acetate extract (EtOAc).…”

Section: Molecular Characterization Of Trichoderma Sp Efi 671mentioning

confidence: 99%

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Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671

et al. 2020

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Botanical and fungal biopesticides, including endophytes, are in high demand given the current restrictive legislations on the use of chemical pesticides. As part of an ongoing search for new biopesticides, a series of fungal endophytes have been isolated from selected medicinal plants including Lauraceae species. In the current study, an extract from the endophytic fungus Trichoderma sp. EFI 671, isolated from the stem parts of the medicinal plant Laurus sp., was screened for bioactivity against plant pathogens (Fusarium graminearum, Rhizoctonia solani, Sclerotinia sclerotiorum and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi) and plant parasites (Meloidogyne javanica), with positive results against M. persicae. The chemical study of the neutral fraction of the active hexane extract resulted in the isolation of a triglyceride mixture (m1), eburicol (2), β-sitostenone (3), ergosterol (4) and ergosterol peroxide (5). The free fatty acids present in the acid fraction of the extract and in m1 (oleic, linoleic, palmitic and stearic) showed strong dose-dependent antifeedant effects against M. persicae. Liquid (potato dextrose broth, PDB and Sabouraud Broth, SDB) and solid (corn, sorghum, pearl millet and rice) growth media were tested in order to optimize the yield and bioactivity of the fungal extracts. Pearl millet and corn gave the highest extract yields. All the extracts from these solid media had strong effects against M. persicae, with sorghum being the most active. Corn media increased the methyl linoleate content of the extract, pearl millet media increased the oleic acid and sorghum media increased the oleic and linoleic acids compared to rice. The antifeedant effects of these extracts correlated with their content in methyl linoleate and linoleic acid. The phytotoxic effects of these extracts against ryegrass, Lolium perenne, and lettuce, Lactuca sativa, varied with culture media, with sorghum being non- toxic.

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Section: Isolation Of Endophytic Fungus Trichoderma Sp Efi 671mentioning

confidence: 99%

Section: Molecular Characterization Of Trichoderma Sp Efi 671mentioning

confidence: 99%

Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671

et al. 2020

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“…Endophytic fungus was isolated from the medicinal plant of Laurus sp. according to Kumar et al, (2013) with surface sterilization method [16]. Surface of stem and leaf was sterilized with 70% ethanol for 2 min followed by 1% sodium hypochlorite for 3 min.…”

Section: Isolation Of Endophytic Fungus Trichoderma Sp Efi 671mentioning

confidence: 99%

“…EFI 671 was cultivated on 200 g of sterilized rice medium in 1000 ml conical flask for metabolites extraction as per the method of Kumar and Kaushik (2013) [18]. After 15 days of growth at 25ºC under dark conditions, metabolites were extracted using 250 ml of ethyl acetate (EtOAc) solvent according Kumar et al, (2013) [16]. The organic solvent was evaporated to dryness in a rotary evaporator (Heidolph, Germany) yielding 500 mg of ethyl acetate extract (EtOAc).…”

Section: Small-scale Cultivationmentioning

confidence: 99%

“…EFI 671 was incubated on 250 g sterilized rice medium in 10 conical flask of 1000 ml volume for 15 days as the same conditions described above for isolation of active compounds. The extraction and partition of fungal extract was done according to Kumar et al (2013) as described above [16]. A total of 6.0 g of hexane extract was obtained from the partition of 13.46 g of EtOAc extract.…”

Section: Large-scale Cultivation For Compound Isolationmentioning

confidence: 99%

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Aphid Antifeedant Fatty Acids from an Endophytic Fungus <em>Trichoderma</em> sp. EFI671

Kaushik¹,

Díaz²,

Chhipa³

et al. 2019

Preprint

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In the current study, an ethyl acetate extract from the endophytic fungus Trichoderma sp. EFI 671, isolated from the stem parts of the medicinal plant Laurus sp. was screened for bioactivity against plant pathogens (Fusarium graminearum, Rhizoctonia solani, Sclerotinia sclerotiorum and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi) and plant parasites (Meloidogyne javanica). The chemical study of this insect antifeedant extract resulted in the isolation of 1-oleoyl-2-linoleoyl-3-palmitoylglycerol (1), eburicol (2), β-sitostenone (3), ergosterol (4) and ergosterol peroxide (5). The free fatty acids present in compound 1 (oleic, linoleic and palmitic) showed strong dose-dependent aphid antifeedant effects against M. persicae. Liquid (PDB, and SDB) and solid (corn, sorghum, pearl millet and rice) growth media were tested in order to optimize the yield and bioactivity of the fungal extracts. Pearl millet and corn gave the highest extract yields. All the extracts from these solid media had strong effects against M. persicae with sorghum being the most active. Corn increased the content in linolenic, pearl millet the oleic and stearic and sorghum oleic and linolenic acids compared to rice. Their antifeedant effects correlated with linoleic /oleic acids. The phytotoxic effects of these extracts against Lolium perenne and Lactuca sativa varied with culture media, with sorghum being the least toxic.

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New holistic approach for the management of biofilm‐associated infections by myco‐metabolites

et al. 2022

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Biofilm‐associated infections have increased excessively over the recent years due to the increased population having impaired immune systems or as a result of certain medical conditions like transplantation, cancer, and any other chronic ailments. The abrupt enhancement of antibiotic resistance and enhanced utilization of biomedical devices offer new opportunities for microbial colonization leading to the development of microbial biofilms. Total eradication of recalcitrant microbial biofilms demands the adoption of a holistic approach and since the fungal metabolites enriched with bioactive compounds show efficacy in inhibiting the multiple factors behind biofilm formation, the anti‐biofilm activities of fungal metabolites need to be appraised. Being effective in preventing various steps of biofilm formation, including inhibition of surface adhesion and cell‐to‐cell communication through quorum quenching, blocking of quorum sensing receptors, and enzymes involved in microbial cell wall biosynthesis, targeting the virulence factors and finally killing of biofilm bound individual cells; myco‐metabolites are found effective as a potent holistic anti‐biofilm agent. The wide spectrum of bioactive substances of fungi and their anti‐biofilm activities against different pathogens and their multitarget characteristics are very promising in the field of treating biofilm infections.

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Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera

Cited by 34 publications

References 16 publications

Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671

Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, Trichoderma sp. EFI671

Aphid Antifeedant Fatty Acids from an Endophytic Fungus <em>Trichoderma</em> sp. EFI671

New holistic approach for the management of biofilm‐associated infections by myco‐metabolites

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