Identification of Antifungal Substance (Iturin A2) Produced by Bacillus subtilis B47 and Its Effect on Southern Corn Leaf Blight

Ye, Yunfeng; Li, Qiqin; Fu, Gang; Yuan, Gonglin; Jin, Miao; Lin, Wei

doi:10.1016/s1671-2927(12)60786-x

Cited by 61 publications

(25 citation statements)

References 41 publications

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“…Antimicrobial activity by most of the biocontrol bacteria is due to the compounds that are commonly reported to be secreted by most of the microorganisms, including lipopeptide families (Yánez-Mendizábal et al 2012;Nihorimbere et al 2012;Viviana et al 2012), antimicrobial peptides, iturin (Ye et al 2012), surfactin (Bacon and Hinton, 2011) and extracellular proteins (Hossain et al 2009). Compared with chemical biocides, many antibiotics produced by antagonistic strains have the advantage of being easily decomposed, leaving no harmful residues (Cook, 1993).…”

Section: Discussionmentioning

confidence: 99%

Identification of ethylparaben as the antimicrobial substance produced by Brevibacillus brevis FJAT-0809-GLX

Che

Liu

Zheng

et al. 2015

Microbiological Research

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In this study, crude antimicrobial extract from the culture supernatant of Brevibacillus brevis FJAT-0809-GLX was extracted, and its antimicrobial activity was investigated with the agar diffusion method. The results showed that the antimicrobial activity of the culture supernatant of B. brevis FJAT-0809-GLX increased with the extension of the incubation time of B. brevis FJAT-0809-GLX. The antimicrobial spectrum assays showed that this crude antimicrobial extract from culture supernatant of B. brevis FJAT-0809-GLX could inhibit the growth of both bacteria and fungi. A heat stability test was performed, and different temperatures (30°C, 50°C and 70°C) did not affect the antibiotic activity of this crude antimicrobial extract. The crude antimicrobial extract was also tolerable to changes in pH levels. Its antibiotic activity against Escherichia coli was stable at pH 1 to pH 11, with zone sizes ranging from 18.46mm to 22.19mm. Almost all of the crude extracts extracted using different solvents showed variable degrees of inhibition zones against E. coli, with zone sizes ranging from 17.29mm to 19.62mm, except petroleum ether and butanol extracts, which were found to be completely inactive. Purification of the antimicrobial components was carried out using a column chromatographic technique with column chromatography grade silica gel and analyzed by an Agilent 7890A Network GC system. The separated compound was identified as ethylparaben, with a retention time of 21.980min and a relative amount of 95.50%. The antimicrobial activity of ethylparaben on different types of bacteria and fungi was investigated, and ethylparaben was shown to inhibit different types of microbes to different extents. To the best of our knowledge, this is the first report demonstrating that the bacterium B. brevis could produce ethylparaben.

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Section: Discussionmentioning

confidence: 99%

Identification of ethylparaben as the antimicrobial substance produced by Brevibacillus brevis FJAT-0809-GLX

Che

Liu

Zheng

et al. 2015

Microbiological Research

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“…Bacillus amyloliquefaciens species (Priest et al, 1987), which belong to Bacillus spp., are known to produce important enzymes and bioactive compounds, and thus have been used as biological control agents and biofertilizer for agriculture (Compant et al, 2005;Lugtenberg and Kamilova, 2009;Ye et al, 2012). Strain L-S60, a PGPR strain, has been classified as B. amyloliquefaciens subsp.…”

mentioning

confidence: 99%

Complete genome sequence of Bacillus amyloliquefaciens L-S60, a plant growth-promoting and antifungal bacterium

Qin

Han

Ya-qiong

et al. 2015

Journal of Biotechnology

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“…Bacterial growth and biomass response to media were found to rely heavily on different composition ranging from single to multiple substrates, although screening and optimization approach of medium constituents is not much popular [3]. Although the nutrient concentrations used in this work were inside the range of values normally used for B. cereus culture media, the cell density was significantly higher at a range greater than the recommended value [29,30]. In addition, the growth rate is observed to be a function of nutrient composition [31], uptake and utilization which is also a function of growth machinery or phase.…”

Section: Effect Of Nutrient Concentrationmentioning

confidence: 92%

Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design

Ahmad¹,

Azoddien²,

Zahari³

et al. 2017

IJBSBT

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Identification of Antifungal Substance (Iturin A2) Produced by Bacillus subtilis B47 and Its Effect on Southern Corn Leaf Blight

Cited by 61 publications

References 41 publications

Identification of ethylparaben as the antimicrobial substance produced by Brevibacillus brevis FJAT-0809-GLX

Identification of ethylparaben as the antimicrobial substance produced by Brevibacillus brevis FJAT-0809-GLX

Complete genome sequence of Bacillus amyloliquefaciens L-S60, a plant growth-promoting and antifungal bacterium

Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design

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