Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes for use in cancer immunotherapy

Ozaki, Yoshitomo; Kontani, Keiichi; Teramoto, Koji; Fujita, Takuya; Tezuka, Noriaki; Sawai, Satoru; Watanabe, Hiroyoshi; Fujino, Shozo; Asai, Tohru; Ohkubo, Iwao

doi:10.1016/j.bbrc.2004.03.155

Cited by 11 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ozaki et al have previously demonstrated that cyt-MAA/LGALS3BP is highly immunogenic, since HLA-A2 restricted epitopes of this antigen are recognized by specific T lymphocytes [48] and anti-cyt-MAA/LGALS3BP-specific antibodies are elevated in cancer patients [49]. Our finding that NB cells express high levels of cyt-MAA/LGALS3BP may be very interesting in the view of a possible use of this antigen as target for T-cell-mediated immunotherapy of NB.…”

Section: Discussionmentioning

confidence: 99%

Serum levels of cytoplasmic melanoma-associated antigen at diagnosis may predict clinical relapse in neuroblastoma patients

Morandi

Corrias

Levreri

et al. 2011

Cancer Immunol Immunother

View full text Add to dashboard Cite

The high molecular weight melanoma-associated antigen (HMW-MAA) and the cytoplasmic melanoma-associated antigen (cyt-MAA/LGALS3BP) are expressed in melanoma. Their serum levels are increased in melanoma patients and correlate with clinical outcome. We investigated whether these molecules can serve as prognostic markers for neuroblastoma (NB) patients. Expression of cyt-MAA and HMW-MAA was evaluated by flow cytometry in NB cell lines, patients’ neuroblasts (FI-NB), and short-term cultures of these latter cells (cNB). LGALS3BP gene expression was evaluated by RT–qPCR on FI-NB, cNB, and primary tumor specimens. Soluble HMW-MAA and cyt-MAA were tested by ELISA. Cyt-MAA and HMW-MAA were expressed in NB cell lines, cNB, and FI-NB samples. LGALS3BP gene expression was higher in primary tumors and cNB than in FI-NB samples. Soluble cyt-MAA, but not HMW-MAA, was detected in NB cell lines and cNBs supernatants. NB patients’ serum levels of both antigens were higher than those of the healthy children. High cyt-MAA serum levels at diagnosis associated with higher incidence of relapse, independently from other known risk factors. In conclusion, both HMW-MAA and cyt-MAA antigens, and LGALS3BP gene, were expressed by NB cell lines and patients’ neuroblasts, and both antigens’ serum levels were increased in NB patients. Elevated serum levels of cyt-MAA at diagnosis correlated with relapse, supporting that cyt-MAA may serve as early serological biomarker to individuate patients at higher risk of relapse that may require a more careful follow-up, after being validated in a larger cohort of patients at different time-points during follow-up. Given its immunogenicity, cyt-MAA may also be a potential target for NB immunotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Serum levels of cytoplasmic melanoma-associated antigen at diagnosis may predict clinical relapse in neuroblastoma patients

Morandi

Corrias

Levreri

et al. 2011

Cancer Immunol Immunother

View full text Add to dashboard Cite

show abstract

“…Cell fraction preparation was followed as described (Renko et al, 1990; Quarto et al, 1991). Rat fibroblasts (in 150 mm dishes) were treated with IFNγ (10 ng/ml) in serum‐free medium for 1, 6, and 24 h. The control group was in serum‐free medium for 24 h only.…”

Section: Methodsmentioning

confidence: 99%

Long‐term clinical and angiographic outcomes after implantation of sirolimus‐eluting stents with a “modified mini‐crush” technique in coronary bifurcation lesions

Yang

Tahk

Woo

et al. 2009

Cathet Cardio Intervent

View full text Add to dashboard Cite

show abstract

“…Indeed, cytotoxic T lymphocytes can be activated by pulsing with peptides derived from Mac-2BP loaded onto antigen-presenting cells (Ozaki et al, 2004b). Thus, immunotherapy with Mac-2BP peptide-pulsed cytotoxic T lymphocytes could become an attractive alternative in the treatment of certain cancers.…”

Section: Mac-2 Binding Protein: a Tumor-associated Antigenmentioning

confidence: 99%

The Conserved Scavenger Receptor Cysteine-Rich Superfamily in Therapy and Diagnosis

Moestrup²,

et al. 2011

View full text Add to dashboard Cite

Identification of antigenic epitopes recognized by Mac-2 binding protein-specific cytotoxic T lymphocytes for use in cancer immunotherapy

Cited by 11 publications

References 19 publications

Serum levels of cytoplasmic melanoma-associated antigen at diagnosis may predict clinical relapse in neuroblastoma patients

Serum levels of cytoplasmic melanoma-associated antigen at diagnosis may predict clinical relapse in neuroblastoma patients

Long‐term clinical and angiographic outcomes after implantation of sirolimus‐eluting stents with a “modified mini‐crush” technique in coronary bifurcation lesions

The Conserved Scavenger Receptor Cysteine-Rich Superfamily in Therapy and Diagnosis

Contact Info

Product

Resources

About