Identification of antimicrobial resistance genes in multidrug-resistant clinical Bacteroides fragilis isolates by whole genome shotgun sequencing

Sydenham, Thomas Vognbjerg; Sóki, Jozsef; Hasman, Henrik; Wang, Mikala; Justesen, Ulrik Stenz

doi:10.1016/j.anaerobe.2014.10.009

Cited by 46 publications

(33 citation statements)

References 34 publications

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“…Our case along with other recent reports of metronidazole and carbapenem resistance in the non-B. thetaiotaomicron B. fragilis group (15,24,26) suggests that carbapenems and metronidazole, once considered to be universally active against B. fragilis group organisms, cannot be assumed to be active today, emphasizing the need for routine identification and antimicrobial susceptibility testing of selected anaerobic bacteria.…”

Section: Discussionmentioning

confidence: 48%

“…A recent report described an imipenem-, metronidazole-, piperacillin-tazobactam-, and clindamycin-resistant Bacteroides genome species strain related to B. fragilis; whole-genome sequencing detected several antimicrobial resistance genes in this isolate, including cfiA13 (with an upstream insertion sequence), cfxA, two putative nim genes, ermF, tetQ, and effluxassociated genes (26). Another recent report described whole-genome sequencing of five meropenem-nonsusceptible B. fragilis strains, three of which were nonsusceptible to metronidazole (15). cfiA was detected in all five, and nim genes were detected in the metronidazole-nonsusceptible isolates; tetQ, bexB, ermF, linAn2, and mefEn2 were variously detected (15).…”

Section: Discussionmentioning

confidence: 85%

“…Metronidazole resistance in the B. fragilis group has been associated with acquisition of nim genes (nimA to nimH and nimJ) located on the chromosome or on plasmids (15). The B. thetaiotaomicron isolate from the aforementioned case carried nimE (2).…”

Section: Discussionmentioning

confidence: 99%

“…We and others have used whole-genome sequencing in an attempt to characterize resistance mechanisms in B. fragilis group members (15,26). A recent report described an imipenem-, metronidazole-, piperacillin-tazobactam-, and clindamycin-resistant Bacteroides genome species strain related to B. fragilis; whole-genome sequencing detected several antimicrobial resistance genes in this isolate, including cfiA13 (with an upstream insertion sequence), cfxA, two putative nim genes, ermF, tetQ, and effluxassociated genes (26).…”

Section: Discussionmentioning

confidence: 99%

“…Another recent report described whole-genome sequencing of five meropenem-nonsusceptible B. fragilis strains, three of which were nonsusceptible to metronidazole (15). cfiA was detected in all five, and nim genes were detected in the metronidazole-nonsusceptible isolates; tetQ, bexB, ermF, linAn2, and mefEn2 were variously detected (15). Whole-genome sequencing currently has limitations for detecting antimicrobial resistance genes in the B. fragilis group.…”

Section: Discussionmentioning

confidence: 99%

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Metronidazole- and Carbapenem-Resistant Bacteroides thetaiotaomicron Isolated in Rochester, Minnesota, in 2014

Sadarangani

Cunningham

Jeraldo

et al. 2015

Antimicrob Agents Chemother

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Emerging antimicrobial resistance in members of the Bacteroides fragilis group is a concern in clinical medicine. Although metronidazole and carbapenem resistance have been reported in Bacteroides thetaiotaomicron, a member of the B. fragilis group, they have not, to the best of our knowledge, been reported together in the same B. thetaiotaomicron isolate. Herein, we report isolation of piperacillin-tazobactam-, metronidazole-, clindamycin-, ertapenem-, and meropenem-resistant B. thetaiotaomicron from a patient with postoperative intra-abdominal abscess and empyema. Whole-genome sequencing demonstrated the presence of nimD with at least a portion of IS1169 upstream, a second putative nim gene, two ␤-lactamase genes (one of which has not been previously reported), two tetX genes, tetQ, ermF, two cat genes, and a number of efflux pumps. This report highlights emerging antimicrobial resistance in B. thetaiotaomicron and the importance of identification and antimicrobial susceptibility testing of selected anaerobic bacteria.

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Section: Discussionmentioning

confidence: 48%