Identification of appressorial and mycelial cell wall proteins and a survey of the membrane proteome of Phytophthora infestans

Grenville‐Briggs, Laura J.; Avrova, Anna O.; Hay, Rebecca J.; Bruce, Catherine; Whisson, Stephen C.; West, Pieter van

doi:10.1016/j.funbio.2010.06.003

Cited by 44 publications

(47 citation statements)

References 72 publications

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“…Over a hundred reference gene models were re-annotated to confirm the presence of a signal peptide. Finally, the comparison of our de novo results, with previous studies in P. infestans, showed a considerable overlap among the identified proteins in the extracellular medium (9,42), and similar results were obtained when comparing our dataset to the extracellular proteome of C. albicans (62).…”

Section: Figsupporting

confidence: 85%

“…Transmembrane containing proteins were previously identified from the oomycete cell walls (42,43). In total, 31 proteins were identified by de novo proteome analysis that contained a signal peptide and a single transmembrane domain.…”

Section: Phospholipase D Activity Release Is Dependent On Mediamentioning

confidence: 99%

“…Studies on cell wall located proteins of P. ramorum and P. infestans revealed the presence of effector proteins or pathogen-associated molecular pattern molecules, either as part of the incorporated or immobilized moiety. In addition, proteins both with and without predicted signal peptide were identified (42,43). Despite these efforts, a more comprehensive overview of the in vivo extracellular proteome of P. infestans, or any other oomycete, is currently lacking.…”

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confidence: 99%

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Profiling the Secretome and Extracellular Proteome of the Potato Late Blight Pathogen Phytophthora infestans

Meijer

Mancuso

Espadas

et al. 2014

Molecular & Cellular Proteomics

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Oomycetes are filamentous organisms that cause notorious diseases, several of which have a high economic impact. Well known is Phytophthora infestans, the causal agent of potato late blight. Previously, in silico analyses of the genome and transcriptome of P. infestans resulted in the annotation of a large number of genes encoding proteins with an N-terminal signal peptide. This set is collectively referred to as the secretome and comprises proteins involved in, for example, cell wall growth and modification, proteolytic processes, and the promotion of successful invasion of plant cells. So far, proteomic profiling in oomycetes was primarily focused on subcellular, intracellular or cell wall fractions; the extracellular proteome has not been studied systematically. Here we present the first comprehensive characterization of the in vivo secretome and extracellular proteome of P. infestans. We have used mass spectrometry to analyze P. infestans proteins present in seven different growth media with mycelial cultures and this resulted in the consistent identification of over two hundred proteins. Gene ontology classification pinpointed proteins involved in cell wall modifications, pathogenesis, defense responses, and proteolytic processes. Moreover, we found members of the RXLR and CRN effector families as well as several proteins lacking an obvious signal peptide. The latter were confirmed to be bona fide extracellular proteins and this suggests that, similar to other organisms, oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment. Molecular & Cellular

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Section: Figsupporting

confidence: 85%

Section: Phospholipase D Activity Release Is Dependent On Mediamentioning

confidence: 99%

mentioning

confidence: 99%

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Profiling the Secretome and Extracellular Proteome of the Potato Late Blight Pathogen Phytophthora infestans

Meijer

Mancuso

Espadas

et al. 2014

Molecular & Cellular Proteomics

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“…RNA Extraction-RNA extraction was performed as described previously (9). Briefly, total RNA was extracted from frozen samples ground in liquid nitrogen using a Qiagen RNeasy Plant Mini kit following the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

“…In two studies, screening of ϳ200 spots allowed identification of a small number of specific proteins that were more abundant during appressorium formation and were involved in amino acid biosynthesis or cellulose synthesis or that exhibited changes in abundance during preinfection stages (7,8). In another study Grenville-Briggs et al (9) specifically analyzed proteins from the cell wall of sporulating mycelium, nonsporulating mycelium and appressoria (9) using LC-MS/MS and could identify four proteins as unique to the P. infestans appressorium cell wall.…”

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confidence: 99%

Proteomic Analysis of Phytophthora infestans Reveals the Importance of Cell Wall Proteins in Pathogenicity

Resjö

Brus

Ali

et al. 2017

Molecular & Cellular Proteomics

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The oomycete Phytophthora infestans is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six P. infestans life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up-or downregulated in different combinations of life stages. One of these profiles contained 59 proteins that were more abundant in germinated cysts and appressoria. A large majority of these proteins were not previously recognized as being appressorial proteins or involved in the infection process. Among those are proteins with putative roles in transport, amino acid metabolism, pathogenicity (including one RXLR effector) and cell wall structure modification. We analyzed the expression of the genes encoding nine of these proteins using RT-qPCR and found an increase in transcript levels during disease progression, in agreement with the hypothesis that these proteins are important in early infection. Among the nine proteins was a group involved in cell wall structure modification and adhesion, including three closely related, uncharacterized proteins encoded by PITG_01131, PITG_01132, and PITG_16135, here denoted Piacwp1-3. Transient silencing of these genes resulted in reduced severity of infection, indicating that these proteins are important for pathogenicity. Our results contribute to further insight into P. infestans biology, and indicate processes that might be relevant for the pathogen while preparing for host cell penetration and during infection. The mass spectrometry data have been deposited to ProteomeXchange via the PRIDE partner repository with the data set identifier PXD002446. Molecular & Cellular

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Plant Infection by Biotrophic Fungal and Oomycete Pathogens

Gan

Dodds

Hardham

2011

Signaling and Communication in Plant Symbiosis

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Identification of appressorial and mycelial cell wall proteins and a survey of the membrane proteome of Phytophthora infestans

Cited by 44 publications

References 72 publications

Profiling the Secretome and Extracellular Proteome of the Potato Late Blight Pathogen Phytophthora infestans

Profiling the Secretome and Extracellular Proteome of the Potato Late Blight Pathogen Phytophthora infestans

Proteomic Analysis of Phytophthora infestans Reveals the Importance of Cell Wall Proteins in Pathogenicity

Plant Infection by Biotrophic Fungal and Oomycete Pathogens

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