Identification of Asian Pear Varieties by SSR Analysis

Kimura, Tetsuya; Shi, Yaoyao; Shoda, M.; Kotobuki, K.; Matsuta, Nagao; Hayashi, Tateki; Ban, Yoshiyuki; Yamamoto, Takatsugu

doi:10.1270/jsbbs.52.115

Cited by 94 publications

(70 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In total, the 17 SSR markers allowed identification of 332 alleles in the 192 examined P. pyraster genotypes. The resultant mean of 19.5 alleles per locus (Table 3) was higher than the 14.8 reported previously for wild pear by Kimura et al [9], 17.3 by Volk et al [16] and 13 by Yakovin et al [19]. The differences may be a result of the loci examined and the large number of individuals for geographically remote populations.…”

Section: Characteristics and Diversity Of Ssr Markersmentioning

confidence: 54%

“…SSRs (simple sequence repeats or microsatellites) have been widely used in plant genetic research due to their high reproducibility, polymorphism, co-dominant character and abundance in plant genomes [2][3][4][5]. SSR markers have been isolated from European pear [6][7][8] as well as from Asian pear species [9,10]. Additionally, a large number of SSRs isolated from apple have revealed transferability to pear [11,12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity and population structure of wild pear (Pyrus pyraster (L.) Burgsd.) in Poland

et al. 2014

View full text Add to dashboard Cite

In order to provide molecular characteristics of wild pear (P. pyraster) resources, six populations (192 accessions) from different regions of Poland were investigated with 17 SSR loci. Each of the SSR loci used was polymorphic, with a mean of 19.5 alleles per locus and a mean PIC of 0.806. Both the high heterozygosity (Ho = 0.751) and low Fis (0.007) indicated that the wild pear populations maintain a relatively high level of diversity, while the mean Findex of 0.039 and the number of migrants per generation (Nm = 6.996) revealed a high gene flow and weak inter-population differentiation. AMOVA analysis located polymorphisms mainly within populations (96%). Genetic relations between populations did not show correlations with geographical distances. The dispersal influence of gene flow could be the reason of the disrupted relationship within populations and the low interpopulation differentiation. We did not find any evidence to support the hypothesis about influence of interspecies hybridization with pear cultivars on the level of wild pear population diversity.

show abstract

Section: Characteristics and Diversity Of Ssr Markersmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of wild pear (Pyrus pyraster (L.) Burgsd.) in Poland

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The developed SSR markers were successfully utilized to construct genetic linkage maps, genetic identification, and parentage analysis in pear (Kimura et al, 2002(Kimura et al, , 2003Yamamoto et al, 2002bYamamoto et al, , 2006. Smulders et al (2000Smulders et al ( , 2003 reported that the 8 simple sequence repeat (SSR) markers developed could be used to evaluate the genetic diversity of Dianthus species and identify carnation varieties.…”

Section: Introductionmentioning

confidence: 99%

Development of SSR Markers in Carnation (Dianthus caryophyllus)

Kimura

Yagi

Nishitani

et al. 2009

J. Japan. Soc. Hort. Sci.

Self Cite

View full text Add to dashboard Cite

Thirteen SSR (simple sequence repeat) markers were successfully developed in carnation using an enriched genomic library method. All 13 primer pairs designed could successfully amplify target fragments of the expected size for the original variety 'Francesco' or 'Barbara'. Thirty-nine of 41 carnation varieties, except for mutant varieties, were successfully distinguishable using 32 putative alleles and 15 polymorphic bands produced from 13 SSR markers. 'U Conn Sim' and 'White Sim', which are bud mutants originated from 'William Sim', showed an identical genotype to 'William Sim'. Three (CB026a, CB057a, and CB060a) of 13 SSR markers were thought to be derived from multi-locus because the 3 SSR markers produced more than 3 discrete amplified fragments. The number of alleles per locus ranged from 1 (CB020a and CB041a) to 9 (CB003a), with an average value of 3.2. The values of the observed heterozygosity (H O ) and the expected heterozygosity (H E ) ranged from 0 to 0.59 (mean 0.30) and 0 to 0.68 (mean 0.34), respectively. The power of discrimination (PD) ranged from 0 (CB020a and CB041a) to 0.93 (CB026a) (mean 0.49). The parentage of 2 carnation varieties from intraspecific crosses was analyzed using 10 SSR markers, and the hybridity of the progenic varieties was confirmed. SSR markers were very effective for variety discrimination and determination of carnation parentage.

show abstract

“…The results for the number of alleles per locus were within the ranges reported for other studies of Pyrus sp. cultivars (Kimura et al, 2002;Fernández-Fernández et al, 2006;Miranda et al, 2010;Dequigiovanni et al, 2012). Heterozygosity summarizes the fundamental variations of genetic diversity in a population (Berg and Harmik, 1997), and is therefore commonly employed in studies of diversity.…”

Section: Resultsmentioning

confidence: 99%