Identification of atractyloside by LC–ESI–MS in alleged herbal poisonings

Steenkamp, Paul A.; Harding, N.M.; Heerden, Fanie R. van; Wyk, B.‐E. Van

doi:10.1016/j.forsciint.2005.11.010

Cited by 34 publications

(18 citation statements)

References 15 publications

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“…Several difficulties arise when considering the self-prescribed use of non-allopathic plant remedies including: the interaction between herbal products with pharmaceutical drugs reviewed by Izzo and Ernst (2001); multiple use of natural products leading to synergistic interactions with cytotoxic and mutagenic effects; failure of patients to disclose their use of alternative medicines with allopathic practitioners and forensic experts (Stewart and Steenkamp, 2000;Steenkamp et al, 2006), and finally the toxicity of medicinal plant treatments (Barnes, 2003;Fennell et al, 2004b). Other hazards encountered when ingesting plant materials are related to horticultural techniques utilised (such as fungicide and pesticide use); and, post-harvest agricultural practices and storage of perishable materials (Fennell et al, 2004a).…”

Section: Traditional Medicine and Natural Products Sector: A Balancinmentioning

confidence: 99%

Current perspectives on an emerging formal natural products sector in South Africa

Makunga

Philander

Smith

2008

Journal of Ethnopharmacology

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Section: Traditional Medicine and Natural Products Sector: A Balancinmentioning

confidence: 99%

Current perspectives on an emerging formal natural products sector in South Africa

Makunga

Philander

Smith

2008

Journal of Ethnopharmacology

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“…An HPLC method was described for the detection of ATR and CATR in a 60 % methanolic extract of A. gummifera roots using an evaporative light scattering detector [33]. An HPLC-DAD method was developed for the determination of ATR and CATR in Fructus Xanthii [34], and finally a LC-ESI-MS method was described for the detection of ATR in C. laureola tubers [35] and for the detection of ATR and CATR in biological matrices [36]. The latter methods were used as a starting point for this study.…”

mentioning

confidence: 99%

Influence of Processing on the Content of Toxic Carboxyatractyloside and Atractyloside and the Microbiological Status of Xanthium sibiricum Fruits (Cangʼerzi)

Nikles

Heuberger

Hilsdorf³

et al. 2015

Planta Med

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The dried ripe fruits of Xanthium sibiricum (Cang'erzi) are used in traditional Chinese medicine for the treatment of nasal congestion, nasal discharge, allergic rhinitis, sinusitis, and wind-cold headaches. Carboxyatractyloside and atractyloside are important constituents of the fruits because these diterpenoid glycosides are responsible for their toxicity. In order to evaluate procedures for reducing the amount of the more toxic carboxyatractyloside, the fruits were dried and heated with different methods. Carboxyatractyloside and atractyloside were analysed by a new reversed-phase high-performance liquid chromatographic method using liquid chromatography-diode array detector-tandem mass spectrometry analysis. The results revealed that temperature and drying methods have a strong influence on the content of carboxyatractyloside and atractyloside. Fruits which were treated at higher temperatures showed a lower content of carboxyatractyloside and an increased content of atractyloside, which is 50 times less toxic. This indicates that the roasting process can reduce toxicity effectively. The microbiological colonisation of Xanthium fruits is also reduced by roasting and by drying above 100 °C. For the safe use of Cang'erzi, the effect of processing should be monitored and analysis of carboxyatractyloside and atractyloside should be obligatory in quality control.

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“…Up until now, there are several methods for determination of ATR or CATR in plants [21][22][23] or biological matrix [24][25][26] such as the gastric washing, viscera samples, blood or urine using TLC, LC-DAD, LC-UV, LC-MS, LC-MS/MS, or GC-MS. Up until now, there are several methods for determination of ATR or CATR in plants [21][22][23] or biological matrix [24][25][26] such as the gastric washing, viscera samples, blood or urine using TLC, LC-DAD, LC-UV, LC-MS, LC-MS/MS, or GC-MS.…”

Section: Introductionmentioning

confidence: 99%

“…First of all, it is important to have an analytical tool to support pharmacokinetic investigation about ATR and CATR. Up until now, there are several methods for determination of ATR or CATR in plants [21][22][23] or biological matrix [24][25][26] such as the gastric washing, viscera samples, blood or urine using TLC, LC-DAD, LC-UV, LC-MS, LC-MS/MS, or GC-MS. However, the simultaneous quantification of ATR and CATR in plasma has been few explored until now.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of atractyloside and carboxyatractyloside in rat plasma by LC‐MS/MS: Application to a pharmacokinetic study after oral administration of Xanthii Fructus extract

Pan

Yang

Xiang

et al. 2019

J of Separation Science

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Xanthii Fructus is extensively used as an herbal medicine. Ingestion of this herb is associated with severe hepatotoxicity and nephrotoxicity. Atractyloside and carboxyatractyloside are two dominative toxic constituents in Xanthii Fructus. However, their pharmacokinetic study is lacking. In this study, a novel high-performance liquid chromatography-tandem mass spectrometry method was developed to simultaneously quantify the rat plasma concentrations of atractyloside and carboxyatractyloside. After protein precipitation, the analytes were chromatographic separated on a ZORBAX Eclipse Plus column (2.1 × 150 mm id, 5 µm) under gradient elute. In the negative electrospray ionization mode, the transitions at m/z 725.3→645.4 for atractyloside, m/z 769.3→689.4 for carboxyatractyloside, and m/z 479.2→121.1 for paeoniflorin (the internal standard) were acquired by multiple reaction monitoring. This analytical method showed good linearity over 1-500 ng/mL for atractyloside and 2-500 ng/mL for carboxyatractyloside with acceptable precision and accuracy. No matrix effect, instability and carryover occurred in the analysis procedure. The extraction recoveries were greater than 85.0%. This method was applied to a preliminary pharmacokinetic study by orally administering Xanthii Fructus extract (9 g/kg) to rats, which was useful to evaluate the role of these two compounds in Xanthii Fructus-induced toxicity. K E Y W O R D Satractyloside, carboxyatractyloside, mass spectrometry, pharmacokinetics, Traditional Chinese medicine 590

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Identification of atractyloside by LC–ESI–MS in alleged herbal poisonings

Cited by 34 publications

References 15 publications

Current perspectives on an emerging formal natural products sector in South Africa

Current perspectives on an emerging formal natural products sector in South Africa

Influence of Processing on the Content of Toxic Carboxyatractyloside and Atractyloside and the Microbiological Status of Xanthium sibiricum Fruits (Cangʼerzi)

Simultaneous quantification of atractyloside and carboxyatractyloside in rat plasma by LC‐MS/MS: Application to a pharmacokinetic study after oral administration of Xanthii Fructus extract

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