Identification of australian arboviruses in inoculated cell cultures using monoclonal antibodies in ELISA

Broom, A.K.; Hall, Roy A.; Johansen, Cheryl A.; Oliveira, Nidia M M; Howard, Megan A.; Lindsay, M.D.; Kay, Brian H.; Mackenzie, John S.

doi:10.1080/00313029800169456

Cited by 84 publications

(60 citation statements)

References 11 publications

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“…Viruses were identified using the tissue culture enzyme immunoassay (TCEIA) described elsewhere. 18 Confluent 96-well monolayers of C6/36 cells were inoculated with stock virus supernatant and incubated for up to five days. Cell monolayers were fixed with PBS/BSA (0.2% w/v) containing acetone (20% v/v).…”

Section: Methodsmentioning

confidence: 99%

“…Monoclonal antibodies used in the assay included 4G2 (flavivirus group reactive), 995 (JE), 8C4 (Murray Valley encephalitis and Alfuy), 2B2 (Kunjin and West Nile), 2E5 (Kokobera and Stratford), 6F7 (Edge Hill and Sepik), 11F4 (alphavirus group reactive), B82A2 (Ross River), 9E8 (Barmah Forest), and 2F2 (Sindbis). [18][19][20][21][22][23] Monoclonal antibodies 11F4, 9E8, and 2F2 were obtained from James Cook University Tropical Biotechnology Pty Ltd (Townsville, Australia). Microtiter plates containing monolayers of C6/36 cells were also infected with reference viruses; these and uninfected C6/36 cell monolayers were included in the TC/EIA as controls.…”

Section: Methodsmentioning

confidence: 99%

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Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Johansen¹,

Hurk²,

Ritchie³

et al. 2000

Am J Trop Med Hyg

104

109

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After Japanese encephalitis (JE) virus emerged in the Torres Strait in Australia in 1995, investigations were initiated into the origin of the incursion. New Guinea was considered the most likely source, given its proximity to islands of the Torres Strait. Almost 400,000 adult mosquitoes were processed for virus isolation from 26 locations in the Western Province of Papua New Guinea (PNG) between February 1996 and February 1998, yielding three isolates of JE virus. Two isolates of Murray Valley encephalitis, 17 isolates of Sindbis, and 1 each of Sepik and Ross River viruses were also obtained. Nucleic acid sequences of the PNG JE isolates were determined in the prM region, and in a region overlapping a part of the fifth nonstructural protein and the 3' untranslated region. The PNG isolates belonged to genotype II, and shared > 99.2% identity with isolates from humans and mosquitoes from the Torres Strait, suggesting that PNG is the source of incursions of JE virus into Australia.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Johansen¹,

Hurk²,

Ritchie³

et al. 2000

Am J Trop Med Hyg

104

109

View full text Add to dashboard Cite

show abstract

“…Mosquitoes were processed using the methods described by Ritchie and others and Broom and others. 5,14 At Queensland Health, mosquito pools were examined for both alphaviruses and flaviviruses after inoculation of homogenized mosquito pools onto monolayers of C6/ 36 cells. After 10 days incubation, cells were harvested and viruses identified by immunofluorescent staining using a panel of monoclonal antibodies.…”

Section: Methodsmentioning

confidence: 99%

Flaviviruses isolated from mosquitoes collected during the first recorded outbreak of Japanese encephalitis virus on Cape York Peninsula, Australia.

Hurk

Johansen²,

Zborowski³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. In response to an outbreak of Japanese encephalitis (JE) virus on Cape York Peninsula, Australia, in 1998, mosquitoes were collected using CO 2 and octenol-baited Centers for Disease Control and Prevention light traps. A total of 35,235 adult mosquitoes, comprising 31 species, were processed for virus isolation. No isolates of JE virus were recovered from these mosquitoes. However, 18 isolates of Kokobera virus, another flavivirus were obtained from Culex annulirostris. Twelve isolates were from western Cape York (minimum infection rate (MIR) of 0.61: 1,000 mosquitoes) and 6 were from the Northern Peninsula Area (MIR of 1.0:1,000). Potential explanations for the failure to detect JE virus in mosquitoes collected from Cape York Peninsula include the timing of collections, the presence of alternative bloodmeal hosts, differences in pig husbandry, asynchronous porcine seroconversion, and the presence of other flaviviruses.

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“…Confirmation of tissue culture virus isolations was made by in situ ELISA using the anti-West Nile NS1 monoclonal antibody 3.1112G. 31,32 Infection data were compared with avian serology results from the same birds as part of the avian surveillance program in Orange County. 33 Dead birds were collected in response to reports from the public and various animal control agencies by phone calls directly to a vector control district, or to the California Department of Public Health (CDPH) Dead Bird Program Hot Line.…”

mentioning

confidence: 99%

Vector-Host Interactions Governing Epidemiology of West Nile Virus in Southern California

Molaei¹,

Cummings²,

Su³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Southern California remains an important focus of West Nile virus (WNV) activity, with persistently elevated incidence after invasion by the virus in 2003 and subsequent amplification to epidemic levels in 2004. Eco-epidemiological studies of vectors-hosts-pathogen interactions are of paramount importance for better understanding of the transmission dynamics of WNV and other emerging mosquito-borne arboviruses. We investigated vector-host interactions and host-feeding patterns of 531 blood-engorged mosquitoes in four competent mosquito vectors by using a polymerase chain reaction (PCR) method targeting mitochondrial DNA to identify vertebrate hosts of blood-fed mosquitoes. Diagnostic testing by cell culture, real-time reverse transcriptase-PCR, and immunoassays were used to examine WNV infection in blood-fed mosquitoes, mosquito pools, dead birds, and mammals. Prevalence of WNV antibodies among wild birds was estimated by using a blocking enzyme-linked immunosorbent assay. Analyses of engorged Culex quinquefasciatus revealed that this mosquito species acquired 88.4% of the blood meals from avian and 11.6% from mammalian hosts, including humans. Similarly, Culex tarsalis fed 82% on birds and 18% on mammals. Culex erythrothorax fed on both birds (59%) and mammals (41%). In contrast, Culex stigmatosoma acquired all blood meals from avian hosts. House finches and a few other mostly passeriform birds served as the main hosts for the blood-seeking mosquitoes. Evidence of WNV infection was detected in mosquito pools, wild birds, dead birds, and mammals, including human fatalities during the study period. Our results emphasize the important role of house finches and several other passeriform birds in the maintenance and amplification of WNV in southern California, with Cx. quinquefasciatus acting as both the principal enzootic and “bridge vector” responsible for the spillover of WNV to humans. Other mosquito species, such as Cx. tarsalis and Cx. stigmatosoma, are important but less widely distributed, and also contribute to spatial and temporal transmission of WNV in southern California.

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Identification of australian arboviruses in inoculated cell cultures using monoclonal antibodies in ELISA

Cited by 84 publications

References 11 publications

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Isolation of Japanese encephalitis virus from mosquitoes (Diptera: Culicidae) collected in the Western Province of Papua New Guinea, 1997-1998.

Flaviviruses isolated from mosquitoes collected during the first recorded outbreak of Japanese encephalitis virus on Cape York Peninsula, Australia.

Vector-Host Interactions Governing Epidemiology of West Nile Virus in Southern California

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